HIGH-THROUGHPUT PLASMA ANALYSIS

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

HIGH-THROUGHPUT PLASMA ANALYSIS

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

Standardized and robust workflows are essential for quantitative protein profiling in large sample cohorts. With pre-set gradients, the Evosep One is easy to operate, while maintaining reproducibility and sensitivity in a high-throughput manner.

Emily Chen, Sr. Director,
Thermo Fisher Precision Medicine Science Center

 

LESS SAMPLE PREP WORKFLOW STEPS

Simplification of workflows are preferred for standardized solutions. The Evotip serves as an integrated purification step, which saves time compared to conventional methods as some time-consuming steps can be omitted, when using the Evosep One.

The procedure is compatible with all upstream sample preparation protocols and for large cohort studies, where samples are often prepared with automated sample processing robots, the 96-well format of the Evotips fits perfectly.

CLINICAL APPLICABILITY TO THE PLASMA PROTEOME

The Evosep One is designed for high throughput applications, with a focus on clinical analysis. Blood plasma is the most widely analyzed clinical matrix, with million of samples drawn daily.

Reproducibility is essential in clinical proteomics and across 96 independent, parallel sample preparations and injections of the same original plasma measured in less than two days of LC-MS time, the correlation over all runs is excellent.

See the results and read more here

STANDARDIZED WORKFLOWS FOR LARGE COHORT PLASMA PROFILING

Thermo Fisher Scientific and Evosep are on a joint mission to develop standardized and scalable plasma protein quantitative profiling workflows. Our recent developments have been featured in an application note by the Precision Medicine Science Centre led by Dr. Emily Chen.

The aim of the study was to develop a standardized, high throughput, and reproducible workflow for bloodbased biomarker discovery using automated sample preparation. The 60 samples per day method was chosen for the study. The results demonstrated 3% CV for retention time shift over hundreds of repeated measurements and <15% CV for peak area quantification while maintaining the sensitivity.

See the results and read full app note here.

PLASMA PROTEOMICS GOES HIGH-THROUGHPUT WITH 100 SAMPLES PER DAY

In collaboration with Roman Fischer, University of Oxford and Bruker Daltonik,  we combined the robustness and speed of the timsTOF Pro with PASEF together with the high throughput LC-separation provided by the Evosep One to analyze 100 ng samples of blood plasma from 192 severe infection patients at a sampling rate of 100 samples per day.

It provides robust quantification of more than 500 proteins in a single run and 772 collectively quantified if all runs are merged together.

See the results and read full app note here.

THE FUTURE OF BLOOD TESTING

Mass spectrometry-based proteomics has become a powerful technology in biological research, emphasizing the need for robust, fast and efficient sample preparation.

Read the full overview of clinical plasma proteomics here.

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More research on plasma

Here you can see publications available on plasma research featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn Morenone_hfilter
Gradient off-set focusing HPLC insturment for robust and high throughput clinical proteomics, , 2017Evosep One for clinical analysis

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A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics, , , 2018Description of EvosepMax Planck Institute of Biochemistry, Martinsried Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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Robust and Reprodusible Protein Quantification in Plasma Using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS, , 2020Agilent feasibility studyAgilent Technologies, United States

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High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIAMax Planck Institute of Biochemistry, Martinsried, Germany,

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Clinical proteomics, 2018Clinical proteomics posterMax Planck Institute of Biochemistry, Martinsried, Germany

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Development of a Novel LC Concept for Clinical Proteomics, , 2018Description of Evosep OneMax Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany

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MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysisBruker Daltonics, Germany

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Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).Bruker Daltonics, Germany

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Consistency, consistency – Automated Sample Prep for Translational Proteomics, 2020In this webinar, Emily Chen, Sr. Director at the Thermo Fisher Precision Medicine Science Center presents an automated, robust and scalable sample preparation pipeline for large-scale clinical research samples.Thermo Fisher Scientific, United States

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An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer, , , , , 2020In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States,

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Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States, ,

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A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, , , 2020This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …Max Planck Institute of Biochemistry, Martinsried, Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.Le Laboratoire des Courses Hippiques (LCH), France

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