Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signaturesIn this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1...
Evosep One facilitates rapid and comprehensive maps of the subcellular proteome and phospho-proteome dynamics
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level.
Improving proteome coverage in short LC gradients
In a collaboration with Thermo Fisher Scientific, the Olsen Group and Novo Nordisk Foundation Center for Protein Research have described the new Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer for high-throughput analyses with short gradients using the Evosep One.