EVOSEP+: Adding specialized applications to a standardized platform

Adding specialized
applications to a
standardized platform

EVOSEP+ ADDS SPECIALIZED APPLICATIONS TO A STANDARDIZED PLATFORM

Here we introduce a new set of methods that extend the potential of the Evosep One platform for clinical research. While expanding our scope for specialized methods, we continue to deliver these with our key values such as ease of use, speed, robustness and sensitivity.

EVOSEP+ is a dynamic environment, which continuously provides simplified solutions to new applications.

robust, very low nanoflow single-cell analysis with the WHISPER 40SPD BETA method

The Whisper 40SPD beta method is ideal for ultra-sensitive applications, such as single cell proteomics, where one of the main challenges is to maintain sensitivity and robustness throughout the workflow.

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ULTRA-SENSITIVE ROUTINE APPLICATIONS with the WHISPER 20 SPD BETA method

The Whisper 20SPD beta method features a longer gradient in a series of methods developed with Whisper Flow Technology.

It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.

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MORE PROTEOME COVERAGE IN A SINGLE EXPERIMENT WITH THE EXTENDED METHOD

This Extended Evosep One method is designed for maximum proteome coverage in a single-shot analysis with an 88 min gradient corresponding to a throughput of 15 samples per day.

The raw identifications allow for 35,000 unique peptides resulting in 5000 proteins from data-dependent acquisition with excellent reproducibility.

See the results and read more here.

Data courtesy: Professor Matthias Mann and Johannes Müller, Max-Planck Institute of Biochemistry, Proteomics and Signal Transduction on a Thermo Scientific Orbitrap Exploris 480 MS

Average protein identifications from five technical replicates with the Extended method.

LARGE SCALE DOPING ANALYSIS WITH THE HIGH ORGANIC METHOD

The High Organic method is specifically developed for targeted analysis of stanozolol in equine urine and hair.

It enables small-molecules analysis of significantly more hydrophobic targets. Thus, the analytes are sequentially eluted of the Evotips with up to 100% acetronitrile. The method has a throughput of 100 samples per day and is designed for our EV1064 column.

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“With a key focus on robustness, speed and standardization, we are proud to introduce EVOSEP+ as a solution for specialized applications for MS-based omics.”,

Nicolai Bache, Head of Applications at Evosep