AN AUTOMATED OPENTRONS LOADING PROTOCOL FOR SAMPLE LOADING OF
EVOTIP PURE

A complete, automated end-to-end sample loading protocol for the Opentrons OT-2 makes use of a layered sandwich approach with defined airgaps between the layers. This sandwich is pushed through the Evotip with the OT-2 pipette for 100 seconds leaving the Evotip ready for injection on the Evosep One.

The automated sample loading protocol relies on a defined sample volume of 20 µl and solvent A volumes of 15 µl and 150 µl respectively to keep the sandwich intact through loading.

The protocol has been converted into an easy-to-use HTML form, that generates a complete python script for use in the Opentrons app, allowing the user to load from 8 to 288 Evotips in a single run.

An Evotip Loading kit (OT-2) (EV1144) is required to run the protocol as well as two pipettes; an Opentrons 8-channel P20 pipette and an Opentrons 8-channel P300 pipette.

Protocols

Opentrons ot-2 Evotip Loading protocol

Opentrons OT-2 Evotip loading protocol allowing you to generate a protocol that suits your needs. The protocol uses the Opentrons 8-channel P20 and P300 pipettes to load eight Evotip Pure at the time and up to 288 Evotip Pure per protocol run.

Generate protocol file and import to Opentrons app.

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