Do you want to know more about who we are and how it all started?

Join us on a walk down memory lane, as we dive into the story behind Evosep.

While we may only be 9(10) years old as a company, the story of the people behind Evosep goes way back. Our founder, Ole Vorm, started working with protein mass spectrometry back in 1989 and founded his first company in 1997. However, he is not the only one at Evosep with a long history within the nanoLC and proteomics community.

Most of our employees have been involved in the field for many years as well, working together with Ole Vorm in different companies long before Evosep saw the light of day. Here, we’ll go through our journey and look at the companies and milestones that led to the birth of Evosep. We’ll also share a bit about the mission of Evosep as well as our methods and newest technology platforms.

The journey towards Evosep

Protana (1997)
Ole Vorm founded his first company Protana in 1997 with the mission to provide the scientific community with a range of “world’s first” proteomics solutions for pharmaceutical and biotechnological research. These solutions included (to mention a few) the first commercial nano-electrospray sources and emitters, as well as the first 2D-gel spot-picking robot.

MDS Proteomics (2000) 

A new chapter began in 2000 when MDS Proteomics acquired Protana. The mission of MDS Proteomics was to make protein-based biomarker discovery the leading factor for discovering new drugs, and we became a key part of the world’s largest proteome analysis platform company with 4 global locations, 300 employees, 60 mass spectrometers and a 600 CPU-cluster for database searches.

Proxeon (2002)

In 2002, a new proteomics adventure emerged, as Proxeon was born out of a management buyout from MDS Proteomics. The overall vision behind Proxeon was to create a new generation of powerful easy-to-use proteomics tools by providing high performance and innovative solutions for medical, pharmaceutical and biotech research. This resulted in the development of a split free nanoLC, which was a rather radical idea at the time. The EASY-nLC™ was different and novel by being a single and fully integrated unit controlled by a touchscreen interface and being preconfigured and optimized for proteomics.

Thermo Scientific™ (2010)

The EASY-nLC proved quite successful and Proxeon was acquired by Thermo Scientific in 2010. This enabled us to continue building on our products, and we introduced a pressure boost to 1000 bar and saw great uptake in EASY-nLC product line. We also invented and designed the Thermo Scientific EASY-Spray ion source with integrated column/emitters as a simple and easy solution for connecting the separation frontend with the mass spectrometer.

Bruker Daltonics (2014-2017)

Our journey took a new turn in 2014, when Bruker offered us the opportunity to design a modernized, split free nanoLC. With more than 10 years of nano-HPLC experience, we designed the Bruker Daltonics nanoElute that launched in 2017.

Evosep and the launch of Evosep One (2017)
During 20 years in the proteomics industry, we have worked with many skilled people and gained valuable experience. This enabled Ole Vorm and team to found Evosep in 2016 and launch the Evosep One to the market at HUPO in Dublin just one and a half years after Evosep was born. Our mission at Evosep is to improve quality of life and patient care by radically innovating how protein based clinical diagnostics are performed. In order to do so, we engage in collaborations with world-leading scientists about developing new technologies and solutions to make sample separation 10 times faster and 100 times more robust than today’s alternatives.

Read more about the Evosep One here.

Making proteomics and single-cell proteomics easy and routine

Standard methods (2017)
At the launch in 2017, we introduced five standard methods spanning different analysis times that made it possible to analyze 30-300 samples per day. The five standard methods enable the Evosep Instrument to cover a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. In 2023, we launched our fastest method with a throughput of 500 samples per day.

Read more about our standard methods here. 

It’s all in the tip (2017-2022)
We can’t mention our methods without also mentioning the Evotip, as the Evotip itself is a very significant contributor to why proteomics and single-cell proteomics has become so easy and routine. The Evotip lets you load a very small amount of sample on the tip that, once immobilized, is extremely stable and stays right where it is. This lets you store the protein complement from a single cell on the tip, until you elute into the instrument. In April 2022 we introduced a new generation of Evotips, called Evotip Pure™. 

Read more about the Evotip here.

EVOSEP+ (2020 – 2024)
At ASMS 2020 Reboot we introduced EVOSEP+, which was the method universe for non-standard or non-proteomics applications that extend the potential of the Evosep Instrument platform for clinical research. The first method is the Extended Method which makes it possible to do around 15 samples per day. It’s the ideal choice when maximum proteome coverage in a single experiment is needed, especially when the amount of starting material is limited. The EVOSEP+ brand was later discontinued and succeeded by our specialized methods. 

Whisper methods (2021)
Whisper Flow Technology was a platform of innovations that enabled true nano-flow separations down to 100, 50, and 25 nL/min. By forming gradients under high-flow, low-pressure conditions and using a single pump to drive the pre-formed gradient through the column, Whisper improved sensitivity and addressed many long-standing challenges in nanoflow LC. The methods used narrow-bore columns and emitters but required no hardware changes to the Evosep One.

Whisper Flow played an important role in advancing low-flow proteomics and laid the groundwork for the Whisper Zoom methods introduced in 2024, which now deliver even higher sensitivity and performance.

New headquarters (2024)
In 2024, we had outgrown our headquarters in Odense C and moved into our new, significantly larger office and production facility to ensure we can continue scaling our innovation toward making proteomics faster and more robust. The building – which previously functioned as a PCR test center during the COVID era – was completely transformed from top to bottom. It now houses state-of-the-art laboratory spaces, expanded production areas, modern meeting rooms, and a bright, spacious office environment designed to support collaboration and growth.

Whisper Zoom methods (2024)
At ASMS 2024 in Anaheim, we introduced the new Whisper Zoom methods – a new generation of Whisper methods delivering exceptionally high sensitivity and reproducibility for advanced proteomics workflows.
These methods succeeds our Whisper methods from 2021 and set a new benchmark for low-flow analysis performance and enables researchers to achieve more detailed and accurate results, thereby supporting the development of proteomics-based diagnostics.

Evosep Eno (2025)
At ASMS 2025 in Baltimore, we unveiled the Evosep Eno – our next-generation LC platform designed to advance proteomics. Building on the success of the Evosep One, the Eno delivers significantly improved performance, robustness, and chromatographic separation, enabling analyses of over 500 samples per day with exceptional reproducibility.

The Evosep Eno represents a major step forward in making large-scale proteomics truly routine. With sharper peaks, higher quantitative accuracy, and a fully standardized workflow, it empowers researchers to process bigger cohorts, generate deeper proteome coverage, and accelerate the development of proteomics-based diagnostics and translational applications. Learn more about the Evosep Eno here. 

At Evosep we are all proud of the launch of truly low-flow nano methods. Ole Vorm says:

“I think personally I’m probably more proud than any because I’ve actually lived through this phase in the 1990’s and 2000’s where we all strived to make nanoflow routine and we just didn’t succeed. Now finally we get to this point when it becomes a completely robust analysis that everybody can do on a day-to-day basis. It’s just wonderful.”

Thank you for joining us on this journey through the history of Evosep. From pioneering proteomics tools in the 1990s to unveiling the Evosep Eno, our path has always been shaped by innovation, collaboration, and a passion for making proteomics accessible, routine, and impactful.

Watch the video and get a deeper insight into our methods as Ole Vorm talks about the essense of Evosep.