EVOTIPS

Evotips are disposable trap columns that speed up sample loading and significantly reduce carry-over

Integrated Purification and Loading

De-salting on disposable tips has become common practice for sample preparation prior to nano-flow LC-MS. But when using the Evosep One, the steps of manually eluting samples, drying them down, and resuspending them are completely omitted.

The racks of rinsed tips are simply placed on top of the Evosep One which elutes the samples from each tip and runs the separation in one integrated procedure. This approach saves time and avoids the perils of drying down low-level samples.

Preparation Protocol

Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads. Download quick guide

WASH: Wash dry tips with 20 μl solvent B and centrifuge for 60 s at 700 g

CONDITION: Soak in 100 μl 1-propanol until all the tips are pale white

EQUILIBRATE:  Equilibrate soaked tips with 20 μl solvent A and centrifuge for 60 s at 700 g

LOAD: Load samples on wet tips (20 μl in solvent A) and centrifuge for 60 s at 700 g

WASH:  Wash tips with 20 μl solvent A and centrifuge for 60 s at 700 g

WET: Transfer 100 μl solvent A and centrifuge tips for 10 s at 700 g to keep tips wet

The Evotips are now ready…

Preparation Protocol

Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads. Download quick guide

WASH: Wash dry tips with 20 μl solvent B and centrifuge for 60 s at 700 g

CONDITION: Soak in 100 μl 1-propanol until all the tips are pale white

EQUILIBRATE:  Equilibrate soaked tips with 20 μl solvent A and centrifuge for 60 s at 700 g

LOAD: Load samples on wet tips (20 μl in solvent A) and centrifuge for 60 s at 700 g

WASH:  Wash tips with 20 μl solvent A and centrifuge for 60 s at 700 g

WET: Transfer 100 μl solvent A and centrifuge tips for 10 s at 700 g to keep tips wet

The Evotips are now ready…

reproducibility

Evotips are manufactured by Evosep with extraordinary focus on obtaining a high and reproducible quality. All materials have been selected for the particular purpose and the manufacturing process itself is tightly controlled as is the final product.

Evotips are sold in racks of 96 tips. You can order as little as one rack of tips from us, but more likely you would want to order 10 racks, 50 racks or 100 racks at a time.

The Evosep One sample tray accommodates up to 6 racks of Evotips at a time.

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Need more information, do not hesitate to contact us.