EVOTIPS

Evotips are disposable trap columns that speed up sample loading and significantly reduce carry-over

Evotip Pure: a New and improved generation of Evotip

Evotip Pure™ is a new generation Evotip developed for high performance. To ensure quality, we have internalized and refined all critical manufacturing and carefully chosen the chemistry.

The design of Evotip Pure is based on five years of customer feedback added to a thorough review of every single aspect of the Evotip.

Integrated Purification and Loading

De-salting on disposable tips has become common practice for sample preparation prior to nano-flow LC-MS. But when using the Evosep One, the steps of manually eluting samples, drying them down, and resuspending them are completely omitted.

The racks of rinsed tips are simply placed on top of the Evosep One which elutes the samples from each tip and runs the separation in one integrated procedure. This approach saves time and avoids the perils of drying down low-level samples.

Better chromatography with Evotip Pure

The new Evotip Pure will not only provide much better chromatographic performance but also ensure improved results and consistency over time.

The improved peak definition and peak width will significantly increase peak capacity and ultimately lead to better performance.

The new Evotips will give you more efficient sample binding, significantly improved sensitivity at low loads and visibly sharper and more symmetric peaks at all loads. Retention times are identical +/- 1 second using the 60 SPD method.

The improved chromatographic performance is shown above where sharper and more symmetric peaks leads to significantly improved peak capacity.

Significantly improved proteome coverage with emphasis on low loads

A significant increase in peptide identifications with the Evotip Pure is observed for all sample loads across a dilution series, but most pronounced at low sample loads.

This enables deeper proteome coverage, with lower CVs, making them the ideal choice when analyzing low sample amounts, e.g. single cell analysis powered by Whisper Flow Technology.

reproducibility

Evotips are manufactured by Evosep with extraordinary focus on obtaining a high and reproducible quality. All materials have been selected for the particular purpose and the manufacturing process itself is tightly controlled as is the final product.

Evotips are sold in racks of 96 tips. You can order as little as one rack of tips from us, but more likely you would want to order 10 racks, 50 racks or 100 racks at a time.

The Evosep One sample tray accommodates up to 6 racks of Evotips at a time.

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Introducing Evotip Pure: a new generation of Evotip – It’s all in the tip

Available on demand

Evotip Pure™ is a new generation Evotip developed for high performance. To ensure quality, we have internalized and refined all critical manufacturing and carefully chosen the chemistry. 

Preparation Protocol

Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads. Download quick guide

RINSE: Wash dry Evotips with 20 μl Solvent B
and centrifuge at 800 g for 60 seconds.

CONDITION: Soak in 100 μl 1-propanol until all the tips are pale white

EQUILIBRATE:  Equilibrate soaked Evotips with 20 μl
Solvent A and centrifuge at 800 g for 60 seconds. 

LOAD: Load samples on wet tips (20 μl in solvent A) and centrifuge for 60 seconds at 800 g

WASH:  Wash tips with 20 μl solvent A and centrifuge for 60 s at 800 g

WET: Transfer 100 μl solvent A and centrifuge tips for 10 s at 800 g to keep tips wet

The Evotips are now ready.

Preparation Protocol

Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads. Download quick guide

RINSE: Wash dry Evotips with 20 μl Solvent B and centrifuge at 800 g for 60 seconds.

CONDITION: Soak in 100 μl 1-propanol until all the tips are pale white

EQUILIBRATE:  Equilibrate soaked tips with 20 μl solvent A and centrifuge for 60 seconds at 800 g

LOAD: Load samples on wet tips (20 μl in solvent A) and centrifuge for 60 s at 800 g

WASH:  Wash tips with 20 μl solvent A and centrifuge for 60 s at 800 g

WET: Transfer 100 μl solvent A and centrifuge tips for 10 s at 800 g to keep tips wet

The Evotips are now ready…

DOWNLOAD OUR MATERIAL

BROCHURE
evosep one: a standardized  separation tool for clinical omics
PROTOCOL
SAMPLE LOADING PROTOCOL FOR EVOTIPS
quick guide
Brochure
Evotip Pure

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