LITERATURE ROOM
explore more and find all publications, product brochures
and presentations with Evosep One
Here you can find the latest publications from our users and collaboration partners and also discover older publications. Use the search function to explore our Literature Room or get inspiration from the tags and categories.
| Title | Subject | Material | Year | Summary | Institute | Evosep method | MS instrumentation | Learn More | doc_tag_hfilter | evosep_method_hfilter | ms_vendor_hfilter |
|---|---|---|---|---|---|---|---|---|---|---|---|
| High-resolution longitudinal serum proteome trajectories in COVID-19 reveal patients-specific seroconversion | Clinical research, Covid-19 | Publication | 2021 | This publication from OmicEra Diagnostics, describes alterations of the serum proteome during COVID-19 using a scalable plasma proteome profiling workflow. It comprises the most detailed longitudinal protein trajectories during hospitalization, based on one of the largest MS-based body fluid proteomics efforts with a total of 720 plasma serum samples. | OmicEra Diagnostics GmbH, Germany. University Hospital, LMU Munich, Germany. University of Copenhagen, Denmark. | 60 SPD | Bruker timsTOF Pro | clinical-research covid-19 | 60-spd | bruker-timstof-pro | |
| The Host Interactome of Spike Expands the Tropism of SARS-CoV-2 | Clinical research, Covid-19, Interactomes | Publication | 2021 | This publication from the Yates lab, investigates the host interactome determining whether a SARS-CoV-2 infection is productive. They find an “S2 only” dependent, alternative infection of additional cell types with SARS-CoV-2 may impact vaccination strategies and provide a molecular explanation for a severe or prolonged progression of disease in select COVID-19 patients. | Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA | 30 SPD | Bruker timsTOF Pro | clinical-research covid-19 interactomes | 30-spd | bruker-timstof-pro | |
| Molecular origin of blood-based infrared fingerprints | Clinical research, Lung, Tissue | Publication | 2021 | This publication led by the Zigman group describes the molecular understanding of infrared molecular fingerprints (IMFs) by examining a prospective clinical study with both IR spectroscopy ad MS-based proteomics. They find the disease-related differences in IMFs of blood sera are dominated by contributions from the protein fractions, rather than metabolites. | Department of Physics, Ludwig Maximilian University of Munich, Germany. Max Planck Institute of Quantum Optics, Garching, Germany. Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry,Germany. Novo Nordisk Foundation Cent | Thermo Q Exactive HF-X | clinical-research lung tissue | thermo-q-exactive-hf-x | |||
| R1441G but not G201S Mutation Enhances LRRK2 Mediated Rab10 phosporylation in human Peripheral blood neutrophils | Clinical research, Parkinson's disease, Targeted workflow | Publication | 2021 | This publication from University of Dundee led by Esther Sammler, describes that in vivo LRRK2 dependent | University of Dundee, UK. Hospital Clínic de Barcelona, Spain. Universitat de Barcelona, Spain. Biodonostia Health Research Institute, Spain. Columbia University Medical Center, New York, USA. The Michael J Fox Foundation for Parkinson's Research, New Yor | 60 SPD | Thermo Q Exactive HF-X | clinical-research parkinsons-disease targeted-workflow | 60-spd | thermo-q-exactive-hf-x | |
| Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution | DIA, FAIMS, Phosphorylation, Spatial proteomics | Publication | 2021 | This publication from the Olsen group at the Novo Nordisk Foundation Center for Protein Research, describes a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. | University of Copenhagen, Denmark. Oslo University Hospital, Norway. | 60 SPD | Thermo Orbitrap Exploris 480 | dia faims phosphorylation spatial-proteomics | 60-spd | thermo-orbitrap-exploris-480 | |
| Basement membrane stiffness determines metastases formation | DDA, Lung, Tissue, TMT | Publication | 2021 | In this study, the Erler group from the BRIC Institute at University of Copenhagen, Denmark identifies Net4 as a key regulator of basement membrane stiffness, a special type of extracellular matrix, which presents the major barrier cancer cells have to overcome multiple times to form metastases. | University of Copenhagen, Denmark. Freie Universität Berlin, Germany. Munich University of Applied Sciences, Germany. Center for Nanoscience-CeNS, Germany. University of Glasgow, UK. Technical University of Denmark, Denmark. KU Leuven, Belgium. Queen Mary | 30 SPD | Thermo Orbitrap Exploris 480 | dda lung tissue tmt | 30-spd | thermo-orbitrap-exploris-480 | |
| Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication | DDA, Structural proteomics | Publication | 2021 | In this study, researchers from the Helmholtz Centre for Infection Research determined the crystal structure of full-length Yersinia pseudotuberculosis CNFY, bacterial single-chain exotoxins that modulate cytokinetic and inflammatory processes through activation of host cell Rho GTPases. They used Evosep One to study the deamidation activity of RhoA. | University of Münster, Germany. Helmholtz Centre for Infection Research, Germany. Deutsches Zentrum für Infektionsforschung, Germany. Technische Universität Braunschweig, Germany. | Bruker timsTOF Pro | dda structural-proteomics | bruker-timstof-pro | |||
| Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling | DDA, Interactomes | Publication | 2021 | A collaboration led by the Ermonval group from Institute Pasteur describes the interactions of structural and non-structural proteins of pathogenic and low or non-pathogenic orthohantaviruses with human type I Interferon signaling. They suggest that the activation of IFN-I is probably not the only antiviral pathway to be counteracted by orthohantaviruses. | Institut Pasteur, France. Ecole Doctorale Complexité du Vivant, France. UMR 1161 Virologie, France. BREED, INRAE, Université Paris-Saclay, France. Université de Paris, France. MRC-University of Glasgow Centre for Virus Research, UK. Institute of Novel and | 30 SPD | Thermo Orbitrap Fusion | dda interactomes | 30-spd | thermo-orbitrap-fusion | |
| Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation | DIA, Single cell, Technology | Publication | 2020 | In this publication, the Mann group in Münich describes their innovative workflow for true single-cell proteomics. They apply a nearly loss-less miniaturized sample preparation workflow to our robust Whisper100 40SPD method and combine it with diaPASEF on a modified timsTOF mass spectrometer for a total of 420 FACS sorted single cells. | Max Planck Institute of Biochemistry, Martinsried, Germany. Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark. Evosep Biosystems, Denmark. Bruker Daltonik, Germany and United States. Helmholtz Zentrum München, Germany. | Whisper100 40 SPD | Bruker timsTOF Pro | Download publication or learn more on our website | dia single-cell technology | whisper100-40-spd | bruker-timstof-pro |
| Fluvastatin mitigates SARS-CoV-2 infection in human lung cells | Clinical research, Covid-19 | Publication | 2020 | Clinical data of patients suffering from COVID-19 have indicated that statin therapy, used to treat high cholesterol, is associated with a better clinical outcome. A collaboration led by Gisa Gerold investigated the effect of statins on SARS-CoV-2 infection in human lung cells and found that fluvastatin inhibited coronavirus infection, while other tested statins did not. | Medical School Hannover, Germany. Helmholtz Centre for Infection Research, Germany. University of Veterinary Medicine Hannover, Germany. Umeå University, Sweden. German Centre for Infection Research (DZIF), Germany. | Extended method | Bruker timsTOF Pro | clinical-research covid-19 | extended-method | bruker-timstof-pro | |
| ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context | IAP-MS, Technology | Publication | 2020 | This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics. | University of Oxford, United Kingdom. | 100 SPD | Bruker timsTOF Pro | iap-ms technology | 100-spd | bruker-timstof-pro | |
| Seroconversion stages COVID19 into distinct pathophysiological states | Clinical research, Covid-19, Plasma | Publication | 2020 | In this study, researchers from University of Colorado led by Joaquin M. Espinosa, found highly variable seroconversion status among hospitalized Covid-19 patients. Their results support the existence of distinct pathophysiological states, with seroconversion status being potentially useful as a surrogate marker of underlying processes. | University of Colorado, United States. | 30 SPD | Bruker timsTOF Pro | clinical-research covid-19 plasma | 30-spd | bruker-timstof-pro | |
| Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms | Parkinson's disease, Targeted workflow | Publication | 2020 | This publication from University of Dundee led by Dario R Alessi, describes how LRRK1, a less studied homologue of LRRK2 regulates growth factor receptor trafficking and osteoclast biology reinforcing that the LRRK enzymes have evolved as major regulators of Rab biology in Parkinson’s disease. | University of Dundee, United Kingdom. Johann Wolfgang Goethe University, Germany. Michael J Fox Foundation for Parkinson’s Research, United States. | 60 SPD | Thermo Q Exactive HF-X | parkinsons-disease targeted-workflow | 60-spd | thermo-q-exactive-hf-x | |
| Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites | Clinical research, Parkinson's disease, Targeted workflow | Publication | 2020 | This publication from University of Dundee led by Dario R Alessi, describes the validation and development of a new, multiplexed targeted assay that enables the relative quantification of the key components of the LRRK2 pathway, defining the impact of LRRK2 inhibitors and Parkinson’s disease causing mutations. | University of Dundee, United States. Medical University of Vienna, Austria. | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | clinical-research parkinsons-disease targeted-workflow | 30-spd 60-spd | thermo-orbitrap-exploris-480 | |
| diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition | diaPASEF, Technology | Publication | 2020 | A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology. | Max Planck Institute of Biochemistry, Martinsried, Germany. Bruker Daltonik, Germany. ETH Zürich, Switzerland. University of Belfast, United Kingdom. University of Toronto, Canada. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website
| diapasef technology | 100-spd 200-spd 60-spd | bruker-timstof-pro |
| High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology, Tissue, TMT | Publication | 2020 | High throughput workflows for proteomics | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | dda dia faims phosphorylation ptm technology tissue tmt | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | |
| Peptidomic analysis of urine from youths with early type 1 diabetes reveals novel bioactivity of uromodulin peptides in vitro | Clinical research, Peptidomics, Targeted workflow, Urine | Publication | 2019 | Urinary peptidomics in early type 1 diabetes | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 60 SPD | Thermo Q Exactive HF-X | clinical-research peptidomics targeted-workflow urine | 60-spd | thermo-q-exactive-hf-x | |
| Tech note: Evosep One: A gradient off-set focusing HPLC instrument for robust and high throughput proteomics | Technology | 2017 | Evosep One descriptive technical note | technology | |||||||
| Evosep One Field Test at the Max-Planck Institute of Biochemistry | Technology | Application note | 2017 | Evosep One case study with Philipp Geyer | technology | ||||||
| Gradient off-set focusing HPLC insturment for robust and high throughput clinical proteomics | Clinical research, Plasma, Technology | Application note | 2017 | Evosep One for clinical analysis | clinical-research plasma technology | ||||||
| Evosep One: A Separation Tool Designed for Clinical Omics | Technology | Brochure | 2019 | Evosep One Brochure | technology | ||||||
| Sample loading protocol for Evotips | Technology | Application note | 2020 | Evosep One Tip loading guide | technology | ||||||
| Introducing the Evosep One – Instrument Overview | Technology | Video | 2017 | Evosep One instrument video | technology | ||||||
| Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination | DDA, Technology | Poster | 2018 | Improving ID rates with APD on Q Exactive HF-X | Thermo Fisher Scientific, United States | 100 SPD | Thermo Q Exactive HF-X | dda technology | 100-spd | thermo-q-exactive-hf-x | |
| New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One | DDA, DIA, FAIMS, Technology | Brochure | 2020 | Brochure of Exploris 480 with Evosep data from piece no 1 | Thermo Fisher Scientific, United States | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | dda dia faims technology | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | |
| Development of a robust and reprodusible method for detection of citrullination in complex samples | Bacteria, Citrullination, DDA, PTM | Poster | 2018 | Detection of citrullination in complex samples | University of Southern Denmark, Denmark | 60 SPD | Thermo Orbitrap Fusion Lumos | bacteria citrullination dda ptm | 60-spd | thermo-orbitrap-fusion-lumos | |
| A tale of two tails – efficient profiling of protein degraders by specific functional and target engagement readouts | CETSA, DDA, TMT | Publication | 2020 | CETSA MS profiling | Pelago Biosciences, Sweden | Thermo Q Exactive HF-X | cetsa dda tmt | thermo-q-exactive-hf-x | |||
| How to get Speed and Depth in yout Host Cell Protein | Biopharma, DDA, HCP | Video | 2018 | Bruker Daltonics, Germany | 60 SPD | Bruker timsTOF Pro | biopharma dda hcp | 60-spd | bruker-timstof-pro | ||
| A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics | DDA, DIA, Plasma, Technology | Publication | 2018 | Description of Evosep | Max Planck Institute of Biochemistry, Martinsried Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 60 SPD | Thermo Q Exactive HF-X | dda dia plasma technology | 60-spd | thermo-q-exactive-hf-x | |
| Online parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer | DDA, Technology | Publication | 2018 | Description of timsTOF | Max Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany | 200 SPD | Bruker timsTOF Pro | dda technology | 200-spd | bruker-timstof-pro | |
| Robust and Reprodusible Protein Quantification in Plasma Using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS | Plasma, Targeted workflow, Technology | Application note | 2020 | Agilent feasibility study | Agilent Technologies, United States | 60 SPD | Agilent 6495 Triple Quadrupole | plasma targeted-workflow technology | 60-spd | agilent-6495-triple-quadrupole | |
| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Plasma, Technology | Poster | 2020 | BoxCar DIA | Max Planck Institute of Biochemistry, Martinsried, Germany | 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | dia plasma technology | 100-spd 30-spd | thermo-orbitrap-exploris-480 | |
| Clinical proteomics | Clinical research, Plasma | Poster | 2018 | Clinical proteomics poster | Max Planck Institute of Biochemistry, Martinsried, Germany | Thermo | clinical-research plasma | thermo | |||
| Development of a Novel LC Concept for Clinical Proteomics | DDA, Plasma, Technology | Poster | 2018 | Description of Evosep One | Max Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany | 60 SPD | Thermo | dda plasma technology | 60-spd | thermo | |
| Increasing proteome coverage using cysteine-specific DIA Mass spectrometry – Cys-DIA | DIA, PTM | Publication | 2020 | DIA on cysteine-specific peptides | University of Southern Denmark | 60 SPD | Thermo Q Exactive HF-X | dia ptm | 60-spd | thermo-q-exactive-hf-x | |
| Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time | DDA, Lung, Tissue, TMT | Video | 2019 | Comparison study with TMT, same coverage in shorter time | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos | dda lung tissue tmt | 30-spd 60-spd | thermo-orbitrap-fusion-lumos | |
| A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism | DDA, TMT | Publication | 2020 | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 60 SPD | Thermo Orbitrap Fusion Lumos | dda tmt | 60-spd | thermo-orbitrap-fusion-lumos | ||
| Improving proteome coverage and peptide identification rates in short LC gradients | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology | Video | 2020 | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | dda dia faims phosphorylation ptm technology | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | ||
| High-throughput proteomics with Evosep One | DDA, DIA, FAIMS, Phosphoproteomics, PTM, Technology | Video | 2020 | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | dda dia faims phosphoproteomics ptm technology | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | ||
| Rapid proteome analyses using the Evosep One | DDA, DIA, Phosphorylation, PTM | Video | 2018 | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 60 SPD | Thermo Q Exactive HF | dda dia phosphorylation ptm | 60-spd | thermo-q-exactive-hf | ||
| Targeted assay development for quantifying protein half-life over a range of mouse tissues | Biopharma, DDA, Targeted workflow | Video | 2019 | Protein turnover | Glaxo Smith Kline, Unites States | 300 SPD | biopharma dda targeted-workflow | 300-spd | |||
| Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development | Biopharma, DDA, HCP | Publication | 2020 | HCP analysis in Biopharma | Glaxo Smith Kline, Unites States | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos | biopharma dda hcp | 30-spd 60-spd | thermo-orbitrap-fusion-lumos | |
| Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine | Bacteria, Clinical research, DIA | Video | 2019 | View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs. | CHU Québec Université Laval Research Center, Canada | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | bacteria clinical-research dia | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | |
| Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis | DDA, TMT | Publication | 2020 | Technical University of Denmark | 30 SPD | Thermo Q Exactive HF-X | dda tmt | 30-spd | thermo-q-exactive-hf-x | ||
| High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients | DIA, Technology | Application note | 2020 | diaPASEF app note | Bruker Daltonics, Germany | 100 SPD, 200 SPD, 300 SPD, 60 SPD | Bruker timsTOF Pro | dia technology | 100-spd 200-spd 300-spd 60-spd | bruker-timstof-pro | |
| MaxQuant software for ion moiblity enhanced shotgun proteomics | DDA, Plasma, Technology | Publication | 2020 | MaxQuant for timsTOF analysis | Bruker Daltonics, Germany | 100 SPD | Bruker timsTOF Pro | dda plasma technology | 100-spd | bruker-timstof-pro | |
| Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min | Clinical research, DDA, Plasma | Application note | 2019 | The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients). | Bruker Daltonics, Germany | 100 SPD | Bruker timsTOF Pro | clinical-research dda plasma | 100-spd | bruker-timstof-pro | |
| Fast and reproducible phosphoproteomics using MagReSyn® Amine and Tim-IMAC HP magnetic beads and the Evosep One | Phosphorylation, PTM, Technology | Application note | 2020 | Read this application note to learn about the automated workflows for sample preparation developed by the Olsen group in collaboration with Resyn Biosciences. Protein digestion and phosphoenrichment is fully automated for up to 96 samples in parallel with the Kingfisher Flex robot from Thermo Scientific. | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 60 SPD | Thermo Orbitrap Exploris 480 | phosphorylation ptm technology | 60-spd | thermo-orbitrap-exploris-480 | |
| Consistency, consistency – Automated Sample Prep for Translational Proteomics | Clinical research, Plasma | Video | 2020 | In this webinar, Emily Chen, Sr. Director at the Thermo Fisher Precision Medicine Science Center presents an automated, robust and scalable sample preparation pipeline for large-scale clinical research samples. | Thermo Fisher Scientific, United States | 60 SPD | Thermo | clinical-research plasma | 60-spd | thermo | |
| An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer | Clinical research, DDA, Lung, Plasma, Serum, Tissue | Application note | 2020 | In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | Thermo Fisher Scientific, United States | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 240 | clinical-research dda lung plasma serum tissue | 30-spd 60-spd | thermo-orbitrap-exploris-240 | |
| Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform | Clinical research, DDA, Lung, Plasma, Serum | Application note | 2019 | In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | Thermo Fisher Scientific, United States | 100 SPD, 30 SPD, 60 SPD | Thermo Q Exactive HF-X | clinical-research dda lung plasma serum | 100-spd 30-spd 60-spd | thermo-q-exactive-hf-x | |
| Advancing Interactomics Workflows with 4D-Proteomics on the timsTOF Pro | DDA, Yeast | Video | 2020 | In this webinar organized by Bruker, André Michaelis from Matthias Mann’s group presents his large-scale yeast interactome study. He describes a robust platform for affinity purification and an LCMS workflow with Evosep One and timsTOF Pro with more than 8000 LCMS runs completed in just 20 weeks. | Max Planck Institute of Biochemistry, Martinsried, Germany | 60 SPD | Bruker timsTOF Pro | dda yeast | 60-spd | bruker-timstof-pro | |
| Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites | DIA, Liver, Lung, Pulldown, Tissue | Publication | 2019 | In this publication, the Olsen Group have combined DIA with short LC gradients to describe how cancer mutations close to tyrosine phosphorylation sites in the EGF receptor rewire signaling pathways by switching protein interactors. The performed 1200 pulldown experiments in just 20 days of LC-MS instrument time. | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 60 SPD | Thermo Q Exactive HF-X | dia liver lung pulldown tissue | 60-spd | thermo-q-exactive-hf-x | |
| timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics | DDA, Technology | Application note | 2018 | Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness. | Bruker Daltonics, Germany | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | dda technology | 100-spd 200-spd 60-spd | bruker-timstof-pro | |
| Evidence of Structural Protein Damage and Membrane Lipid Remodeling in Red Blood Cells from COVID-19 Patients | Clinical research, Covid-19, DDA | Publication | 2020 | In this study, researchers from University of Colorado led by Angelo D’Alessandro, performed proteomics analysis of Covid-19 patients and healthy control individuals. They measured the proteomes of red blood cells (RBC) and identified increased glycolysis in RBCs from COVID-19 patients, accompanied by increased oxidation of key structural proteins. | University of Colorado, United States | Bruker timsTOF Pro | clinical-research covid-19 dda | bruker-timstof-pro | |||
| Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens | Clinical research, Covid-19, DDA, Targeted workflow | Publication | 2020 | This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2. | Mayo Clinic Rochester, United States | 100 SPD, 200 SPD | Thermo Orbitrap Exploris 480 | clinical-research covid-19 dda targeted-workflow | 100-spd 200-spd | thermo-orbitrap-exploris-480 | |
| Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV | Clinical research, Covid-19, DIA, PTM | Publication | 2020 | This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2. | Max Planck Institute of Biochemistry, Martinsried, Germany | 30 SPD, 60 SPD | Thermo Q Exactive HF-X | clinical-research covid-19 dia ptm | 30-spd 60-spd | thermo-q-exactive-hf-x | |
| Serum Proteomics in COVID-19 Patients: Altered Coagulation and Complement Status as a Function of IL-6 Level | Clinical research, Covid-19, DDA, Serum | Publication | 2020 | This publication describes a serum proteomics analysis of COVID-19 patients, stratified by the degree of inflammation, represented by IL-6 levels. It highlights an increase in the levels of inhibitory components of the fibrinolytic cascade in severe COVID-19 disease, providing potential therapeutic interventions, such as the use of pro-fibrinolytic agents. | University of Colorado, US | Bruker timsTOF Pro | clinical-research covid-19 dda serum | bruker-timstof-pro | |||
| A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease | Clinical research, DIA, Liver, Plasma | Publication | 2020 | This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, … | Max Planck Institute of Biochemistry, Martinsried, Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 30 SPD | Thermo Q Exactive HF-X | clinical-research dia liver plasma | 30-spd | thermo-q-exactive-hf-x | |
| Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time | Offline fractionation, Tissue, TMT | Video | 2019 | Comparison study with TMT, same coverage in shorter time | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos |
| offline-fractionation tissue tmt | 30-spd 60-spd | thermo-orbitrap-fusion-lumos |
| Increasing Throughput: From Pre-Clinical Models To Protein Complexes | DIA, Large-scale | Video | 2019 | Comparison study with >400 samples, same coverage in shorter time | ETH Zürich, Switzerland | 60 SPD | Sciex | dia large-scale | 60-spd | sciex | |
| In-Depth Characterization of Staurosporine Induced Proteome Thermal Stability Changes | CETSA, DDA, Offline fractionation, TMT | Publication | 2020 | Deep CETSA MS profiling of Staurosporine | Pelago Biosciences, Sweden | Thermo Q Exactive HF | cetsa dda offline-fractionation tmt | thermo-q-exactive-hf | |||
| Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles | DDA, Lung, Tissue | Publication | 2020 | In vivo study of biocompatibility of magnetic nanoparticles. Toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined in vivo by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC−MS/MS. | University of Southern Denmark | 60 SPD | Thermo Orbitrap Fusion Lumos | dda lung tissue | 60-spd | thermo-orbitrap-fusion-lumos | |
| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, FAIMS, Offline fractionation, Phosphoproteomics, PTM, Technology, Tissue, TMT | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho. | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | automation dda dia faims offline-fractionation phosphoproteomics ptm technology tissue tmt | 100-spd 200-spd 60-spd | thermo-orbitrap-exploris-480 | |
| Covalent Protein Painting Reveals Structural Changes In The Proteome In Alzheimer Disease | Alzheimer Disease, Brain, DDA, Tissue | Publication | 2020 | Development of Covalent Protein Painting (CPP), a structural proteomics approach to quantify changes in protein fold or altered protein-protein interaction for any protein in a proteome. CPP directly determines the relative surface accessibility of amino acid side chains by measuring the molar fraction of a chemical functionality that is accessible for chemical modification on the … | Scripps Research Institute, United States | 30 SPD | Thermo Orbitrap Fusion Lumos | alzheimer-disease brain dda tissue | 30-spd | thermo-orbitrap-fusion-lumos | |
| Advanced Host Cell Protein (Hcp) Analysis With Tims Qtof Ms Powers Biopharmaceutical Development | Antibodies, DDA, HCP | Publication | 2019 | Descriptive piece of HCP analysis on the timsTOF | Bruker Daltonics, Germany | 60 SPD | Bruker timsTOF Pro | antibodies dda hcp | 60-spd | bruker-timstof-pro | |
| Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry | Bacteria, Targeted workflow | Publication | 2019 | “Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases. | Erasmus MC, The Netherlands | 100 SPD | Thermo Q Exactive HF | bacteria targeted-workflow | 100-spd | thermo-q-exactive-hf | |
| Study: Increased De Novo Protein Sequencing Coverage With Optimal Protease Cocktail | Antibodies, DDA | Poster | 2019 | Increased De Novo Protein Sequencing Coverage with Optimal Protease Cocktail | Rapid Novor, Canada | Thermo Orbitrap Fusion | antibodies dda | thermo-orbitrap-fusion | |||
| High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition | Phosphorylation, PTM, Targeted workflow | Poster | 2018 | High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway. | Thermo Fisher Scientific, United States | 100 SPD, 200 SPD, 60 SPD | Thermo Q Exactive HF-X | phosphorylation ptm targeted-workflow | 100-spd 200-spd 60-spd | thermo-q-exactive-hf-x | |
| Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control | Plasma, rHuEPO, Targeted workflow | Application note | 2019 | This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing. | Le Laboratoire des Courses Hippiques (LCH), France | 100 SPD | Thermo Q Exactive HF | plasma rhuepo targeted-workflow | 100-spd | thermo-q-exactive-hf |
