LITERATURE ROOM

explore more and find all publications, product brochures

and presentations with Evosep One

Here you can find the latest publications from our users and collaboration partners and also discover older publications. Use the search function to explore our Literature Room or get inspiration from the tags and categories.

TitleSubjectMaterialYearSummaryAuthorsOrganizationEvosep methodMS instrumentationLearn Moredoc_tag_hfilterevosep_method_hfilterms_vendor_hfilter
A fleet of Evosep One instruments supports a proteomics factory at Rapid Novor2021Read how instrument-to-instrument standardization played a vital role in the creation of de novo sequencing workflows at Rapid Novor.Evosep

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High Sensitivity Evotips2021For more efficient peptide recovery of small sample loads (< ~100 ng), Evosep recommends High Sensitivity Evotips, that have superior performance at very low loads and almost identical retention times for other applications.Evosep

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Unleashing the power of DIA combined with short gradients using Evosep One2021In this webinar we will dive into the exciting research possibilities that comes by using DIA combined with short gradients with Evosep One.Evosep

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Towards a Standardized Omics Platform with the 30 samples per day method2019This is the longest of our standard methods with a 44-minute gradient and a cycle time of 48 minutes.Evosep

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technology30-spd
Towards a Standardized Omics Platform with the 60 samples per day method2020This method has a 21-minute gradient and a cycle time of 24 minutes. The method is designed for our EV-1064 column and we highly recommend to use this column with our range of emitters and spray adapters for optimal performance.Evosep

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technology60-spd
Towards a Standardized Omics Platform with the 100 samples per day method2019This method has an 11.5 minute gradient and a cycle time of 14.4 minutes.Evosep

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technology100-spd
Towards a Standardized Omics Platform with the 200 samples per day method2019This method has a 5.6 minute gradient and a cycle time of 7.2 minutes. It is designed for our EV-1107 column.Evosep

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technology200-spd
Towards a Standardized Omics Platform with the 300 samples per day method2020This is the fastest of our standard proteomics methods with a throughput of 300 samples per day.Evosep

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technology300-spd
Unleashing the true power of DIA/SWATH data acquisition with short gradients – Rapid profiling of pre-clinical models and proteome dynamics, , 2019Achieving sufficient statistical power for the analysis of a comprehensive set of target proteins requires processing of a large number of samples. With finite resources, researchers face a difficult choice: should they aim at deeper proteomic coverage or higher sample throughput?Evosep

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clinical-research dia technology
Improve and enhance your proteome coverage with the Performance column2019We have developed an additional Performance column for the 60 and 100 samples per day methods (SPD) to address applications with high demands.Evosep,

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technology100-spd 60-spd
The proteomics dilemma – High throughput analysis versus proteome depth2019With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.Evosep, , , ,

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technology100-spd 200-spd 30-spd 300-spd 60-spd
Evosep One: A Separation Tool Designed for Clinical Omics2019Evosep aims to improve quality of life and patient care by radically innovating protein-based clinical diagnostics. This brochure introduces our instrument Evosep One.Evosep

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technology
Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis, 2021In this study, the Palancade group provides the complete depiction of the co-translational events involved in the biogenesis of a large multiprotein assembly, the nuclear pore complex (NPC) in yeast. Their data reveal that distinct, spatially segregated modes of co-translational interactions foster the ordered assembly of NPC subunits.Ophélie Lautier, Arianna Penzo, Jérôme Rouvière, Guillaume Chevreux, Louis Collet, Isabelle Loïodice, Angela Taddei, Frédéric Devaux, Martine A. Collart & Benoit PalancadeUniversité de Paris, Paris, France. Sorbonne Université, Paris, France. University of Geneva, Geneva, Switzerland.

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dda protein-interaction30-spd
Hemorrhage and saline resuscitation are associated with epigenetic and proteomic reprogramming in the rat lung, , 2021In this study, the Sillesen group investigates the alteration of the proteome in the context of hemorrhage and saline resuscitation in trauma patients. They used rat lungs as a model and pooled 10 rats in one combined TMT experiment subjected to high pH offline fractionation.Alexander Bonde, Trine G. Eskesen, Jacob Steinmetz, Erwin M. Schoof, Lene H.D. Blicher, Lars S. Rasmussen & Martin SillesenRigshospitalet, University of Copenhagen, Denmark. Technical University of Denmark, Lyngby, Denmark.

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dda lung tissue30-spdthermo-orbitrap-fusion
Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids, , 2021The Van Eyk group presents a standardized workflow for precise high-throughput proteomics of blood biofluids using the 60 SPD method. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood.Angela Mc Ardle, Aleksandra Binek, Annie Moradian, Blandine Chazarin Orgel, Alejandro Rivas, Kirstin E. Washington, Conor Phebus, Danica-Mae Manalo, James Go, Vidya Venkatraman, Casey Johnson, Qin Fu, Susan Cheng, Koen Raedschelders, Justyna-Fert Bober, SCedars-Sinai Medical Center, Los Angeles, CA, United States. University College Dublin, Dublin, Ireland.

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clinical-research dia plasma60-spdthermo-orbitrap-exploris-480
Malaria Parasite Schizont Egress Antigen-1 Plays an Essential Role in Nuclear Segregation during Schizogony, , 2021This publication by the Blackman group describes the role of SEA1, a suggested malaria vaccine antigen candidate. They conclude that SEA1 does not play a direct mechanistic role in egress to invade new erythrocytes but instead is an essential blood-stage protein that plays an important role in orchestrating the correct partitioning of DNA into merozoites.Abigail J. Perrin, Claudine Bisson, Peter A. Faull, Matthew J. Renshaw, Rebecca A. Lees, Roland A. Fleck, Helen R. Saibil, Ambrosius P. Snijders, David A. Baker & Michael J. BlackmanThe Francis Crick Institute, London, UK. University of London, London, UK. King’s College London, London, UK. London School of Hygiene & Tropical Medicine, London, UK

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dda malaria protein-interaction30-spdthermo-orbitrap-fusion-lumos
The COVIDome Explorer Researcher Portal, , , 2021Researchers from University of Colorado led by Joaquin M. Espinosa, have created a user-friendly researcher portal enabling easy access and real-time analysis of matched multi-omics datasets for COVID-19, termed the COVIDome Explorer. They illustrate the use through a multi-omics analysis of biosignatures associated with C-reactive protein (CRP), an established marker of poor prognosis in COVID-19.Kelly D. Sullivan, Matthew D. Galbraith, Kohl T. Kinning, Kyle Bartsch, Nik Levinsky, Paula Araya, Keith P. Smith, Ross E. Granrath, Jessica R. Shaw, Ryan Baxter, Kimberly R. Jordan, Seth Russell, Monika Dzieciatkowska, Julie A. Reisz, Fabia Gamboni, FranUniversity of Colorado, CO, USA. University of Colorado Anschutz Medical Campus, Aurora, CO, USA. Colorado School of Public Health, Aurora, CO, USA.

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clinical-research covid-19 dda plasma30-spdbruker-timstof-pro
High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe, 2021The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method.Vadim Demichev, Fengchao Yu, Guo Ci Teo, Lukasz Szyrwiel, George A. Rosenberger, Jens Decker, Stephanie Kaspar-Schoenefeld, Kathryn S. Lilley, Michael Mülleder, Alexey I. Nesvizhskii & Markus RalserThe Francis Crick Institute, London, UK. Charité – Universitätsmedizin Berlin, Germany. University of Cambridge, UK. University of Michigan, Ann Arbor, USA. Columbia University, New York, USA. Bruker Daltonics, Bremen, Germany, ,

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diapasef technology-development100-spd 200-spd 60-spdbruker-timstof-pro
A robust mass spectrometer for precision medicine – the Orbitrap Exploris 240 mass spectrometer for large-scale plasma protein profiling, 2021This application note from Thermo Fisher Scientific Precision Medicine Science Center demonstrates the reliability and robustness of the Evosep One coupled with the Orbitrap Exploris 240 MS for large-scale, untargeted plasma protein profiling. They observed excellent reproducibility of protein and peptide identifications over 100 injections of human serum performed five weeks apart.Michelle L Dubuke, Sitara Chauhan, Sarah Trusiak & Emily I. ChenThermo Fisher Scientific, United States

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dda plasma60-spdthermo-orbitrap-exploris-240
Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers, , , 2021In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker.Christoph H Mayr, Lukas M Simon, Gabriela Leuschner, Meshal Ansari, Janine Schniering, Philipp E Geyer, Ilias Angelidis, Maximilian Strunz, Pawandeep Singh, Nikolaus Kneidinger, Frank Reichenberger, Edith Silbernagel, Stephan Böhm, Heiko Adler, Michael LiHelmholtz Zentrum München, Munich, Germany. Ludwig‐Maximilians University (LMU) Munich, Munich, Germany. University Hospital Zurich, Zurich, Switzerland. Max Planck Institute of Biochemistry, Martinsried, Germany. Asklepios Fachkliniken Munich‐Gauting, Mu,

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clinical-research dia fibrosis plasma100-spd 60-spdthermo-q-exactive-hf
Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease, , 2021In this study, the Cader group from University of Oxford, presents a global proteomic analysis of the extracellular matrix (ECM) in mouse and human brains. They report the identification of 147 core ECM proteins of the human brain vascular matrisome, including collagens, laminins, fibronectin and nidogens. Network analysis revealed the connection between the ECM proteins …Alexandra Pokhilko, Gaia Brezzo, Lahiru Handunnetthi, Raphael Heilig, Rachel Lennon, Colin Smith, Stuart M Allan, Alessandra Granata, Sanjay Sinha, Tao Wang, Hugh S Markus, Alexandra Naba, Roman Fischer, Tom Van Agtmael, Karen Horsburgh & M. Zameel CaderUniversity of Oxford, UK. University of Edinburgh, UK. University of Glasgow. UK. University of Manchester, UK. Manchester Academic Health Science Centre, UK. Cambridge Biomedical Campus, Hills Road, UK. University of Illinois at Chicago, USA.

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brain clinical-research dda60-spdbruker-timstof-pro
Evotips: Quick guide2020Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads.Evosep

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Rapid, robust and reproducible host-cell protein analysis with Evosep One2021In this webinar, we will explore the possibilities of using the Evosep One technology to perform rapid, robust and reproducible HCP analysis. In Biopharmaceutical drug development and production, Host Cell Proteins are the leftovers of the cells used to produce the therapeutic protein in the final formulation. Regulatory authorities have increased their demands on the …Evosep, AstraZeneca, Novozymes

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hcp
Covid-19 research with Evosep One2021The global emergence of novel coronavirus disease 2019 (COVID-19) has underlined the requirement for rapid assays with high sensitivity and specificity for detection of infection and further to understand the molecular complexity of the disease. In this webinar you will meet Evosep One users who have used our technology in Covid-19 assay development and to …Evosep, Cedars Sinai, Newcastle University

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covid-19
A beginner’s guide to Evosep2021If you are new to our technology or just getting started this event is for you. We will introduce the basics of the Evosep One technology and include hot-from-the-lab data from our users. Nicolai Bache, Head of Applications at Evosep, will host the event, bringing you up to speed with the latest additions to the Evosep …Evosep, Pelago Bioscience, University of Dundee

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technology
Whisper is coming2021Whisper is the latest Evosep One innovation that allow plug-and-play, split-free 100 nl/min performance. The new methods make use of Evosep’s unique low pressure gradient formation and very accurate flow control on the same standard system without technical modifications.Evosep

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whisper100-40-spd
EVOSEP+2020On June 3rd at ASMS 2020, we launched EVOSEP+ which is a software and application addition to the Evosep One platform. The new set of methods extend the potential for clinical research.Evosep

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extended-method
Powerful pairing of technologies and analytical approaches, 2019View full webinar with Thomas Angel from the Exploratory Biomarker Assay group at GSK presenting his latest data and hands on experience using Evosep One.Evosep, GlaxoSmithKline

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technology tissue
Whisper™ step-by-step guide2021Spray stability is essential for ultra-sensitive proteomics. The highest sensitivity at low flow rates is achieved with the EVOSEP+ Whisper methods.Evosep

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whisper100-40-spd
SPIN – Species by Proteome Investigation, 2021This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication.Patrick L. Rüther, Immanuel M. Husic, Pernille Bangsgaard, Kristian M. Gregersen, Pernille Pantmann, Milena Carvalho, RitaM. Godinho, Lukas Friedl, Joao Cascalheira, Marie Louise S. Jørkov, Michael Benedetti, Jonathan Haws, Nuno Bicho, Frido Welker, EnricUniversity of Copenhagen, Denmark. University of Algarve, Portugal. University of Louisville, USA. University of North Carolina, USA. University of West Bohemia, Czech Republic. University of New Mexico, Albuquerque, USA. Museum Nordsjælland, Denmark. Roy, ,

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ancient-proteomics dia100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
High-resolution longitudinal serum proteome trajectories in COVID-19 reveal patients-specific seroconversion, 2021This publication from OmicEra Diagnostics, describes alterations of the serum proteome during COVID-19 using a scalable plasma proteome profiling workflow. It comprises the most detailed longitudinal protein trajectories during hospitalization, based on one of the largest MS-based body fluid proteomics efforts with a total of 720 plasma serum samples.Philipp E. Geyer, Florian M. Arend, Sophia Doll, Marie-Luise Louiset, Sebastian Virreira Winter, Johannes B. Müller-Reif, Furkan M. Torun, Michael Weigand, Peter Eichhorn, Mathias Bruegel, Maximilian T. Strauss, Lesca M. Holdt, Matthias Mann & Daniel TeupOmicEra Diagnostics GmbH, Germany. University Hospital, LMU Munich, Germany. University of Copenhagen, Denmark.

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clinical-research covid-1960-spdbruker-timstof-pro
The Host Interactome of Spike Expands the Tropism of SARS-CoV-2, , 2021This publication from the Yates lab, investigates the host interactome determining whether a SARS-CoV-2 infection is productive. They find an “S2 only” dependent, alternative infection of additional cell types with SARS-CoV-2 may impact vaccination strategies and provide a molecular explanation for a severe or prolonged progression of disease in select COVID-19 patients.Casimir Bamberger, Sandra Pankow, Salvador Martínez-Bartolomé, Jolene Diedrich, Robin Park & John Yates IIIDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA

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clinical-research covid-19 protein-interaction30-spdbruker-timstof-pro
Molecular origin of blood-based infrared fingerprints, , 2021This publication led by the Zigman group describes the molecular understanding of infrared molecular fingerprints (IMFs) by examining a prospective clinical study with both IR spectroscopy ad MS-based proteomics. They find the disease-related differences in IMFs of blood sera are dominated by contributions from the protein fractions, rather than metabolites.Liudmila Voronina, Cristina Leonardo, Johannes B. Mueller-Reif, Philipp E. Geyer, Marinus Huber, Michael Trubetskov, Kosmas V. Kepesidis, Jürgen Behr, Matthias Mann, Ferenc Krausz & Mihaela ŽigmanDepartment of Physics, Ludwig Maximilian University of Munich, Germany. Max Planck Institute of Quantum Optics, Garching, Germany. Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry,Germany. Novo Nordisk Foundation Cent

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clinical-research lung tissuethermo-q-exactive-hf-x
R1441G but not G201S Mutation Enhances LRRK2 Mediated Rab10 phosporylation in human Peripheral blood neutrophils, , 2021This publication from University of Dundee led by Esther Sammler, describes that in vivo LRRK2 dependentYing Fan, Raja S. Nirujogi, Alicia Garrido, Javier Ruiz Martínez, Alberto Bergareche-Yarza, Elisabet Mondragón Rezola, Ana Vinagre Aragón, Ioana Croitoru, Ana Gorostidi Pagola, Laura Paternain Markinez, Roy Alcalay, Richard A. Hickman, Jonas Duering, NeriUniversity of Dundee, UK. Hospital Clínic de Barcelona, Spain. Universitat de Barcelona, Spain. Biodonostia Health Research Institute, Spain. Columbia University Medical Center, New York, USA. The Michael J Fox Foundation for Parkinson's Research, New Yor

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clinical-research parkinsons-disease targeted-workflow60-spdthermo-q-exactive-hf-x
Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution, , , 2021This publication from the Olsen group at the Novo Nordisk Foundation Center for Protein Research, describes a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions.Ana Martinez-Val, Dorte B. Bekker-Jensen, Sophia Steigerwald, Adi Mehta, Trung Tran, Krzysztof Sikorski, Estefanía Torres-Vega, Ewa Kwasniewicz, Sólveig Hlín Brynjólfsdóttir, Lisa B. Frankel, Rasmus Kjøbsted, Nicolai Krogh, Alicia Lundby, Simon Bekker-JenUniversity of Copenhagen, Denmark. Oslo University Hospital, Norway.

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dia faims phosphorylation spatial-proteomics60-spdthermo-orbitrap-exploris-480
Basement membrane stiffness determines metastases formation, , , 2021In this study, the Erler group from the BRIC Institute at University of Copenhagen, Denmark identifies Net4 as a key regulator of basement membrane stiffness, a special type of extracellular matrix, which presents the major barrier cancer cells have to overcome multiple times to form metastases.Raphael Reuten, Sina Zendehroud, Monica Nicolau, Lutz Fleischhauer, Anu Laitala, Stefanie Kiderlen, Denise Nikodemus, Lena Wullkopf, Sebastian Rune Nielsen, Sarah McNeilly, Carina Prein, Maria Rafaeva, Erwin M. Schoof, Benjamin Furtwängler, Bo T. Porse, HUniversity of Copenhagen, Denmark. Freie Universität Berlin, Germany. Munich University of Applied Sciences, Germany. Center for Nanoscience-CeNS, Germany. University of Glasgow, UK. Technical University of Denmark, Denmark. KU Leuven, Belgium. Queen Mary

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dda lung tissue tmt30-spdthermo-orbitrap-exploris-480
Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication, 2021In this study, researchers from the Helmholtz Centre for Infection Research determined the crystal structure of full-length Yersinia pseudotuberculosis CNFY, bacterial single-chain exotoxins that modulate cytokinetic and inflammatory processes through activation of host cell Rho GTPases. They used Evosep One to study the deamidation activity of RhoA.Paweena Chaoprasid, Peer Lukat, Sabrina Mühlen, Thomas Heidler, Emerich‐Mihai Gazdag, Shuangshuang Dong, Wenjie Bi, Christian Rüter, Marco Kirchenwitz, Anika Steffen, Lothar Jänsch, Theresia E B Stradal, Petra Dersch & Wulf BlankenfeldtUniversity of Münster, Germany. Helmholtz Centre for Infection Research, Germany. Deutsches Zentrum für Infektionsforschung, Germany. Technische Universität Braunschweig, Germany.

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dda structural-proteomicsbruker-timstof-pro
Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling, 2021A collaboration led by the Ermonval group from Institute Pasteur describes the interactions of structural and non-structural proteins of pathogenic and low or non-pathogenic orthohantaviruses with human type I Interferon signaling. They suggest that the activation of IFN-I is probably not the only antiviral pathway to be counteracted by orthohantaviruses.Giulia Gallo, Grégory Caignard, Karine Badonnel, Guillaume Chevreux, Samuel Terrier, Agnieszka Szemiel, Gleyder Roman-Sosa, Florian Binder, Quan Gu, Ana Da Silva Filipe, Rainer G. Ulrich, Alain Kohl, Damien Vitour, Noël Tordo & Myriam ErmonvalInstitut Pasteur, France. Ecole Doctorale Complexité du Vivant, France. UMR 1161 Virologie, France. BREED, INRAE, Université Paris-Saclay, France. Université de Paris, France. MRC-University of Glasgow Centre for Virus Research, UK. Institute of Novel and

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dda protein-interaction30-spdthermo-orbitrap-fusion
Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation, , 2020In this publication, the Mann group in Münich describes their innovative workflow for true single-cell proteomics. They apply a nearly loss-less miniaturized sample preparation workflow to our robust Whisper100 40SPD method and combine it with diaPASEF on a modified timsTOF mass spectrometer for a total of 420 FACS sorted single cells.Andreas-David Brunner, Marvin Thielert, Catherine Vasilopoulou, Constantin Ammar, Fabian Coscia, Andreas Mund, Ole B. Horning, Nicolai Bache, Amalia Apalategui, Markus Lubeck, Oliver Raether, Melvin A. Park, Sabrina Richter, David S. Fischer, Fabian J. ThMax Planck Institute of Biochemistry, Martinsried, Germany. Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark. Evosep Biosystems, Denmark. Bruker Daltonik, Germany and United States. Helmholtz Zentrum München, Germany.

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dia single-cell technologywhisper100-40-spdbruker-timstof-pro
Fluvastatin mitigates SARS-CoV-2 infection in human lung cells, 2020Clinical data of patients suffering from COVID-19 have indicated that statin therapy, used to treat high cholesterol, is associated with a better clinical outcome. A collaboration led by Gisa Gerold investigated the effect of statins on SARS-CoV-2 infection in human lung cells and found that fluvastatin inhibited coronavirus infection, while other tested statins did not.Francisco J. Zapatero-Belinchón, Rebecca Moeller, Lisa Lasswitz, Marco van Ham, Miriam Becker, Graham Brogden, Ebba Rosendal, Wenjie Bi, Belén Carriquí, Koushikul Islam, Annasara Lenman, Antonia P. Gunesch, Jared Kirui, Thomas Pietschmann, Anna K Överby,Medical School Hannover, Germany. Helmholtz Centre for Infection Research, Germany. University of Veterinary Medicine Hannover, Germany. Umeå University, Sweden. German Centre for Infection Research (DZIF), Germany.

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clinical-research covid-19extended-methodbruker-timstof-pro
ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context, 2020This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics.Hannah Jones, Raphael Heilig, Roman Fischer, Benedikt M Kessler & Adan Pinto-FernandezUniversity of Oxford, United Kingdom.

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iap-ms technology100-spdbruker-timstof-pro
Seroconversion stages COVID19 into distinct pathophysiological states, , 2020In this study, researchers from University of Colorado led by Joaquin M. Espinosa, found highly variable seroconversion status among hospitalized Covid-19 patients. Their results support the existence of distinct pathophysiological states, with seroconversion status being potentially useful as a surrogate marker of underlying processes.Matthew D. Galbraith, Kohl T. Kinning, Kelly D. Sullivan, Ryan Baxter, Paula Araya, Kimberly R. Jordan, Seth Russell, Keith P. Smith, Ross E. Granrath, Jessica Shaw, Monika Dzieciatkowska, Tusharkanti Ghosh, Andrew A. Monte, Angelo D’Alessandro, Kirk C. HUniversity of Colorado, United States.

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clinical-research covid-19 plasma30-spdbruker-timstof-pro
Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms, 2020This publication from University of Dundee led by Dario R Alessi, describes how LRRK1, a less studied homologue of LRRK2 regulates growth factor receptor trafficking and osteoclast biology reinforcing that the LRRK enzymes have evolved as major regulators of Rab biology in Parkinson’s disease.Asad U. Malik, Athanasios Karapetsas, Raja S. Nirujogi, Sebastian Mathea, Prosenjit Pal, Pawel Lis, Matthew Taylor, Elena Purlyte, Robert Gourlay, Mark Dorward, Simone Weidlich, Rachel Toth, Nicole K. Polinski, Stefan Knapp, Francesca Tonelli & Dario R AlUniversity of Dundee, United Kingdom. Johann Wolfgang Goethe University, Germany. Michael J Fox Foundation for Parkinson’s Research, United States.

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parkinsons-disease targeted-workflow60-spdthermo-q-exactive-hf-x
Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites, , 2020This publication from University of Dundee led by Dario R Alessi, describes the validation and development of a new, multiplexed targeted assay that enables the relative quantification of the key components of the LRRK2 pathway, defining the impact of LRRK2 inhibitors and Parkinson’s disease causing mutations.Raja S Nirujogi, Francesca Tonelli, Matthew Taylor, Pawel Lis, Alexander Zimprich, Esther Sammler & Dario R AlessiUniversity of Dundee, United States. Medical University of Vienna, Austria.,

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clinical-research parkinsons-disease targeted-workflow30-spd 60-spdthermo-orbitrap-exploris-480
diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition, 2020A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology.Florian Meier, Andreas-David Brunner, Max Frank, Annie Ha, Isabell Bludau, Eugenia Voytik, Stephanie Kaspar-Schoenefeld, Markus Lubeck, Oliver Raether, Nicolai Bache, Ruedi Aebersold, Ben C. Collins, Hannes L. Röst & Matthias MannMax Planck Institute of Biochemistry, Martinsried, Germany. Bruker Daltonik, Germany. ETH Zürich, Switzerland. University of Belfast, United Kingdom. University of Toronto, Canada., ,

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diapasef technology100-spd 200-spd 60-spdbruker-timstof-pro
High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA, , , , , , , 2020High throughput workflows for proteomicsNovo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphorylation ptm technology tissue tmt100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Peptidomic analysis of urine from youths with early type 1 diabetes reveals novel bioactivity of uromodulin peptides in vitro, , , 2019Urinary peptidomics in early type 1 diabetesSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada

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clinical-research peptidomics targeted-workflow urine60-spdthermo-q-exactive-hf-x
Tech note: Evosep One: A gradient off-set focusing HPLC instrument for robust and high throughput proteomics2017Evosep One descriptive technical note

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technology
Evosep One Field Test at the Max-Planck Institute of Biochemistry2017Evosep One case study with Philipp Geyer

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technology
Gradient off-set focusing HPLC insturment for robust and high throughput clinical proteomics, , 2017Evosep One for clinical analysis

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clinical-research plasma technology
Sample loading protocol for Evotips2020Evosep One Tip loading guide

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technology
Introducing the Evosep One – Instrument Overview2017Evosep One instrument video

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technology
Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination, 2018Improving ID rates with APD on Q Exactive HF-XThermo Fisher Scientific, United States

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dda technology100-spdthermo-q-exactive-hf-x
New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One, , , 2020Brochure of Exploris 480 with Evosep data from piece no 1Thermo Fisher Scientific, United States, ,

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dda dia faims technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Development of a robust and reprodusible method for detection of citrullination in complex samples, , , 2018Detection of citrullination in complex samplesUniversity of Southern Denmark, Denmark

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bacteria citrullination dda ptm60-spdthermo-orbitrap-fusion-lumos
A tale of two tails – efficient profiling of protein degraders by specific functional and target engagement readouts, , 2020CETSA MS profilingPelago Biosciences, Sweden

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cetsa dda tmtthermo-q-exactive-hf-x
How to get Speed and Depth in yout Host Cell Protein, , 2018Bruker Daltonics, Germany

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biopharma dda hcp60-spdbruker-timstof-pro
A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics, , , 2018Description of EvosepMax Planck Institute of Biochemistry, Martinsried Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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dda dia plasma technology60-spdthermo-q-exactive-hf-x
Online parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer, 2018Description of timsTOFMax Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany

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dda technology200-spdbruker-timstof-pro
Robust and Reprodusible Protein Quantification in Plasma Using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS, , 2020Agilent feasibility studyAgilent Technologies, United States

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plasma targeted-workflow technology60-spdagilent-6495-triple-quadrupole
High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIAMax Planck Institute of Biochemistry, Martinsried, Germany,

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dia plasma technology100-spd 30-spdthermo-orbitrap-exploris-480
Clinical proteomics, 2018Clinical proteomics posterMax Planck Institute of Biochemistry, Martinsried, Germany

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clinical-research plasmathermo
Development of a Novel LC Concept for Clinical Proteomics, , 2018Description of Evosep OneMax Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany

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Increasing proteome coverage using cysteine-specific DIA Mass spectrometry – Cys-DIA, 2020DIA on cysteine-specific peptidesUniversity of Southern Denmark

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Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time, , , 2019Comparison study with TMT, same coverage in shorter timeSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada,

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dda lung tissue tmt30-spd 60-spdthermo-orbitrap-fusion-lumos
A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism, 2020SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada

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dda tmt60-spdthermo-orbitrap-fusion-lumos
Improving proteome coverage and peptide identification rates in short LC gradients, , , , , 2020Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphorylation ptm technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
High-throughput proteomics with Evosep One, , , , , 2020Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphoproteomics ptm technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Rapid proteome analyses using the Evosep One, , , 2018Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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dda dia phosphorylation ptm60-spdthermo-q-exactive-hf
Targeted assay development for quantifying protein half-life over a range of mouse tissues, , 2019Protein turnoverGlaxo Smith Kline, Unites States

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Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development, , 2020HCP analysis in BiopharmaGlaxo Smith Kline, Unites States,

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biopharma dda hcp30-spd 60-spdthermo-orbitrap-fusion-lumos
Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine, , 2019View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.  CHU Québec Université Laval Research Center, Canada, ,

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Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis, 2020Technical University of Denmark

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High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  Bruker Daltonics, Germany, , ,

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MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysisBruker Daltonics, Germany

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dda plasma technology100-spdbruker-timstof-pro
Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).Bruker Daltonics, Germany

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clinical-research dda plasma100-spdbruker-timstof-pro
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Tim-IMAC HP magnetic beads and the Evosep One, , 2020Read this application note to learn about the automated workflows for sample preparation developed by the Olsen group in collaboration with Resyn Biosciences. Protein digestion and phosphoenrichment is fully automated for up to 96 samples in parallel with the Kingfisher Flex robot from Thermo Scientific.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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phosphorylation ptm technology60-spdthermo-orbitrap-exploris-480
Consistency, consistency – Automated Sample Prep for Translational Proteomics, 2020In this webinar, Emily Chen, Sr. Director at the Thermo Fisher Precision Medicine Science Center presents an automated, robust and scalable sample preparation pipeline for large-scale clinical research samples.Thermo Fisher Scientific, United States

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clinical-research plasma60-spdthermo
An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer, , , , , 2020In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States,

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clinical-research dda lung plasma serum tissue30-spd 60-spdthermo-orbitrap-exploris-240
Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States, ,

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clinical-research dda lung plasma serum100-spd 30-spd 60-spdthermo-q-exactive-hf-x
Advancing Interactomics Workflows with 4D-Proteomics on the timsTOF Pro, 2020In this webinar organized by Bruker, André Michaelis from Matthias Mann’s group presents his large-scale yeast interactome study. He describes a robust platform for affinity purification and an LCMS workflow with Evosep One and timsTOF Pro with more than 8000 LCMS runs completed in just 20 weeks.Max Planck Institute of Biochemistry, Martinsried, Germany

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Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites, , , , 2019In this publication, the Olsen Group have combined DIA with short LC gradients to describe how cancer mutations close to tyrosine phosphorylation sites in the EGF receptor rewire signaling pathways by switching protein interactors. The performed 1200 pulldown experiments in just 20 days of LC-MS instrument time.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics, 2018Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness.Bruker Daltonics, Germany, ,

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dda technology100-spd 200-spd 60-spdbruker-timstof-pro
Evidence of Structural Protein Damage and Membrane Lipid Remodeling in Red Blood Cells from COVID-19 Patients, , 2020In this study, researchers from University of Colorado led by Angelo D’Alessandro, performed proteomics analysis of Covid-19 patients and healthy control individuals. They measured the proteomes of red blood cells (RBC) and identified increased glycolysis in RBCs from COVID-19 patients, accompanied by increased oxidation of key structural proteins.University of Colorado, United States

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clinical-research covid-19 ddabruker-timstof-pro
Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens, , , 2020This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2.Mayo Clinic Rochester, United States,

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clinical-research covid-19 dda targeted-workflow100-spd 200-spdthermo-orbitrap-exploris-480
Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV, , , 2020This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2.Max Planck Institute of Biochemistry, Martinsried, Germany,

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clinical-research covid-19 dia ptm30-spd 60-spdthermo-q-exactive-hf-x
Serum Proteomics in COVID-19 Patients: Altered Coagulation and Complement Status as a Function of IL-6 Level, , , 2020This publication describes a serum proteomics analysis of COVID-19 patients, stratified by the degree of inflammation, represented by IL-6 levels. It highlights an increase in the levels of inhibitory components of the fibrinolytic cascade in severe COVID-19 disease, providing potential therapeutic interventions, such as the use of pro-fibrinolytic agents.University of Colorado, US

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clinical-research covid-19 dda serumbruker-timstof-pro
A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, , , 2020This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …Max Planck Institute of Biochemistry, Martinsried, Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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clinical-research dia liver plasma30-spdthermo-q-exactive-hf-x
Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time, , 2019Comparison study with TMT, same coverage in shorter timeSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada,

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Increasing Throughput: From Pre-Clinical Models To Protein Complexes, 2019Comparison study with >400 samples, same coverage in shorter timeETH Zürich, Switzerland

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In-Depth Characterization of Staurosporine Induced Proteome Thermal Stability Changes, , , 2020Deep CETSA MS profiling of StaurosporinePelago Biosciences, Sweden

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Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles, , 2020In vivo study of biocompatibility of magnetic nanoparticles. Toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined in vivo by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC−MS/MS.University of Southern Denmark

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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Covalent Protein Painting Reveals Structural Changes In The Proteome In Alzheimer Disease, , , 2020Development of Covalent Protein Painting (CPP), a structural proteomics approach to quantify changes in protein fold or altered protein-protein interaction for any protein in a proteome. CPP directly determines the relative surface accessibility of amino acid side chains by measuring the molar fraction of a chemical functionality that is accessible for chemical modification on the …Scripps Research Institute, United States

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Advanced Host Cell Protein (Hcp) Analysis With Tims Qtof Ms Powers Biopharmaceutical Development , , 2019Descriptive piece of HCP analysis on the timsTOFBruker Daltonics, Germany

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Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry, 2019“Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases.Erasmus MC, The Netherlands

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Study: Increased De Novo Protein Sequencing Coverage With Optimal Protease Cocktail, 2019Increased De Novo Protein Sequencing Coverage with Optimal Protease CocktailRapid Novor, Canada

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High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition, , 2018High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway.Thermo Fisher Scientific, United States, ,

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phosphorylation ptm targeted-workflow100-spd 200-spd 60-spdthermo-q-exactive-hf-x
Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.Le Laboratoire des Courses Hippiques (LCH), France

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plasma rhuepo targeted-workflow100-spdthermo-q-exactive-hf