Evosep Literature Room

Evosep Literature room

Welcome to the Evosep Literature Room, your shortcut to our knowledge base. Here, you can find the latest material presented by Evosep and our collaboration partners and also discover older publications and videos.

Featured PUBLICATIONS

Accurate Detection of the Four Most Prevalent Carbapenemases in E. coli and K. pneumoniae by High-Resolution Mass Spectrometry

A team at Erasmus MC have developed a new method to accurately and rapidly detect specific resistance mechanisms simultaneously by combining high-resolution mass spectrometry with liquid chromatography.

Dimard E. Foudraine, Lennard J. M. Dekker, Nikolaos Strepis, Michiel L. Bexkens, Corné H. W. Klaassen, Theo M. Luider, Wil H. F. Goessens

Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

The need for scalable methods for functional annotation of phosphotyrosine sites has led the Olsen group, Novo Nordisk Foundation Center for Protein Research in Copenhagen, to develop a new mass spectrometry-based workflow for high-throughput analyses.  

Authors: Alicia Lundby, Giulia Franciosa, Kristina B. Emdal, Jan C. Refsgaard, Sebastian P. Gnosa, Dorte B. Bekker-Jensen, Anna Secher, Svetlana R. Maurya, Indranil Paul, Blanca L. Mendez, Christian D. Kelstrup, Chiara Francavilla, Marie Kveiborg, Guillermo Montoya, Lars J. Jensen, Jesper V. Olsen 5

Rapid Host Cell Protein (HCP) Detection with Advanced
Trapped Ion Mobility Spectrometry (TIMS) Technology

The robust and reproducible 21 min gradient on the Evosep One instrument provides rapid analysis of downstream processes, with fast runs and high throughput, enabling batch-to-batch fingerprinting. Quantitation is often necessary and the faster analysis with Bruker’s timsTOF Pro is advantageous for more routine applications with coverage of 50+ HCPs at 1% false discovery rate (FDR). 

Authors: Guillaume Tremintin, Stuart Pengelley, Michael Greig

TECH NOTE: EVOSEP ONE: A GRADIENT OFF-SET FOCUSING UHPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT PROTEOMICS

Evosep One has been designed specifically to address and eliminate the prevalent challenges associated with throughput and robustness of nano-flow LC-MS workflows while maintaining sufficient sensitivity and resolving power for clinical omics applications.

Authors: Nicolai Bache, Ole Hoerning, Lasse Falkenby and Ole Vorm

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