LITERATURE ROOM

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and presentations with Evosep One

Here you can find the latest publications from our users and collaboration partne and also discover older publications. Use the search function to explore our Literature Room or get inspiration from the tags and categories.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn Moredoc_tag_hfilterevosep_method_hfilterms_vendor_hfilter
High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA, , , , , , , 2020High throughput workflows for proteomicsNovo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphorylation ptm technology tissue tmt100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Peptidomic analysis of urine from youths with early type 1 diabetes reveals novel bioactivity of uromodulin peptides in vitro, , , 2019Urinary peptidomics in early type 1 diabetesSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada

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clinical-research peptidomics targeted-workflow urine60-spdthermo-q-exactive-hf-x
Tech note: Evosep One: A gradient off-set focusing HPLC instrument for robust and high throughput proteomics2017Evosep One descriptive technical note

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technology
Evosep One Field Test at the Max-Planck Institute of Biochemistry2017Evosep One case study with Philipp Geyer

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technology
Gradient off-set focusing HPLC insturment for robust and high throughput clinical proteomics, , 2017Evosep One for clinical analysis

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clinical-research plasma technology
Evosep One: A Separation Tool Designed for Clinical Omics2019Evosep One Brochure

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technology
Sample loading protocol for Evotips2020Evosep One Tip loading guide

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technology
Introducing the Evosep One – Instrument Overview2017Evosep One instrument video

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technology
Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination, 2018Improving ID rates with APD on Q Exactive HF-XThermo Fisher Scientific, United States

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dda technology100-spdthermo-q-exactive-hf-x
New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One, , , 2020Brochure of Exploris 480 with Evosep data from piece no 1Thermo Fisher Scientific, United States, ,

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dda dia faims technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Development of a robust and reprodusible method for detection of citrullination in complex samples, , , 2018Detection of citrullination in complex samplesUniversity of Southern Denmark, Denmark

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bacteria citrullination dda ptm60-spdthermo-orbitrap-fusion-lumos
A tale of two tails – efficient profiling of protein degraders by specific functional and target engagement readouts, , 2020CETSA MS profilingPelago Biosciences, Sweden

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cetsa dda tmtthermo-q-exactive-hf-x
How to get Speed and Depth in yout Host Cell Protein, , 2018Bruker Daltonics, Germany

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biopharma dda hcp60-spdbruker-timstof-pro
A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics, , , 2018Description of EvosepMax Planck Institute of Biochemistry, Martinsried Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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dda dia plasma technology60-spdthermo-q-exactive-hf-x
Online parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer, 2018Description of timsTOFMax Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany

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dda technology200-spdbruker-timstof-pro
Robust and Reprodusible Protein Quantification in Plasma Using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS, , 2020Agilent feasibility studyAgilent Technologies, United States

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plasma targeted-workflow technology60-spdagilent-6495-triple-quadrupole
High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIAMax Planck Institute of Biochemistry, Martinsried, Germany,

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dia plasma technology100-spd 30-spdthermo-orbitrap-exploris-480
Clinical proteomics, 2018Clinical proteomics posterMax Planck Institute of Biochemistry, Martinsried, Germany

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clinical-research plasmathermo
Development of a Novel LC Concept for Clinical Proteomics, , 2018Description of Evosep OneMax Planck Institute of Biochemistry, Martinsried and Bruker Daltonics, Germany

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Increasing proteome coverage using cysteine-specific DIA Mass spectrometry – Cys-DIA, 2020DIA on cysteine-specific peptidesUniversity of Southern Denmark

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dia ptm60-spdthermo-q-exactive-hf-x
Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time, , , 2019Comparison study with TMT, same coverage in shorter timeSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada,

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dda lung tissue tmt30-spd 60-spdthermo-orbitrap-fusion-lumos
A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism, 2020SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada

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dda tmt60-spdthermo-orbitrap-fusion-lumos
Improving proteome coverage and peptide identification rates in short LC gradients, , , , , 2020Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphorylation ptm technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
High-throughput proteomics with Evosep One, , , , , 2020Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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dda dia faims phosphoproteomics ptm technology100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Rapid proteome analyses using the Evosep One, , , 2018Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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dda dia phosphorylation ptm60-spdthermo-q-exactive-hf
Targeted assay development for quantifying protein half-life over a range of mouse tissues, , 2019Protein turnoverGlaxo Smith Kline, Unites States

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biopharma dda targeted-workflow300-spd
Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development, , 2020HCP analysis in BiopharmaGlaxo Smith Kline, Unites States,

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biopharma dda hcp30-spd 60-spdthermo-orbitrap-fusion-lumos
Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine, , 2019View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.  CHU Québec Université Laval Research Center, Canada, ,

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bacteria clinical-research dia100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis, 2020Technical University of Denmark

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dda tmt30-spdthermo-q-exactive-hf-x
High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  Bruker Daltonics, Germany, , ,

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dia technology100-spd 200-spd 300-spd 60-spdbruker-timstof-pro
MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysisBruker Daltonics, Germany

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dda plasma technology100-spdbruker-timstof-pro
Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).Bruker Daltonics, Germany

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clinical-research dda plasma100-spdbruker-timstof-pro
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Tim-IMAC HP magnetic beads and the Evosep One, , 2020Read this application note to learn about the automated workflows for sample preparation developed by the Olsen group in collaboration with Resyn Biosciences. Protein digestion and phosphoenrichment is fully automated for up to 96 samples in parallel with the Kingfisher Flex robot from Thermo Scientific.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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phosphorylation ptm technology60-spdthermo-orbitrap-exploris-480
Consistency, consistency – Automated Sample Prep for Translational Proteomics, 2020In this webinar, Emily Chen, Sr. Director at the Thermo Fisher Precision Medicine Science Center presents an automated, robust and scalable sample preparation pipeline for large-scale clinical research samples.Thermo Fisher Scientific, United States

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clinical-research plasma60-spdthermo
An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer, , , , , 2020In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States,

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clinical-research dda lung plasma serum tissue30-spd 60-spdthermo-orbitrap-exploris-240
Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.Thermo Fisher Scientific, United States, ,

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clinical-research dda lung plasma serum100-spd 30-spd 60-spdthermo-q-exactive-hf-x
Advancing Interactomics Workflows with 4D-Proteomics on the timsTOF Pro, 2020In this webinar organized by Bruker, André Michaelis from Matthias Mann’s group presents his large-scale yeast interactome study. He describes a robust platform for affinity purification and an LCMS workflow with Evosep One and timsTOF Pro with more than 8000 LCMS runs completed in just 20 weeks.Max Planck Institute of Biochemistry, Martinsried, Germany

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dda yeast60-spdbruker-timstof-pro
Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites, , , , 2019In this publication, the Olsen Group have combined DIA with short LC gradients to describe how cancer mutations close to tyrosine phosphorylation sites in the EGF receptor rewire signaling pathways by switching protein interactors. The performed 1200 pulldown experiments in just 20 days of LC-MS instrument time.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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dia liver lung pulldown tissue60-spdthermo-q-exactive-hf-x
timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics, 2018Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness.Bruker Daltonics, Germany, ,

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dda technology100-spd 200-spd 60-spdbruker-timstof-pro
Evidence of Structural Protein Damage and Membrane Lipid Remodeling in Red Blood Cells from COVID-19 Patients, , 2020In this study, researchers from University of Colorado led by Angelo D’Alessandro, performed proteomics analysis of Covid-19 patients and healthy control individuals. They measured the proteomes of red blood cells (RBC) and identified increased glycolysis in RBCs from COVID-19 patients, accompanied by increased oxidation of key structural proteins.University of Colorado, United States

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clinical-research covid-19 ddabruker-timstof-pro
Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens, , , 2020This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2.Mayo Clinic Rochester, United States,

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clinical-research covid-19 dda targeted-workflow100-spd 200-spdthermo-orbitrap-exploris-480
Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV, , , 2020This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2.Max Planck Institute of Biochemistry, Martinsried, Germany,

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clinical-research covid-19 dia ptm30-spd 60-spdthermo-q-exactive-hf-x
Serum Proteomics in COVID-19 Patients: Altered Coagulation and Complement Status as a Function of IL-6 Level, , , 2020This publication describes a serum proteomics analysis of COVID-19 patients, stratified by the degree of inflammation, represented by IL-6 levels. It highlights an increase in the levels of inhibitory components of the fibrinolytic cascade in severe COVID-19 disease, providing potential therapeutic interventions, such as the use of pro-fibrinolytic agents.University of Colorado, US

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clinical-research covid-19 dda serumbruker-timstof-pro
A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, , , 2020This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …Max Planck Institute of Biochemistry, Martinsried, Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

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clinical-research dia liver plasma30-spdthermo-q-exactive-hf-x
Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time, , 2019Comparison study with TMT, same coverage in shorter timeSickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada,

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offline-fractionation tissue tmt30-spd 60-spdthermo-orbitrap-fusion-lumos
Increasing Throughput: From Pre-Clinical Models To Protein Complexes, 2019Comparison study with >400 samples, same coverage in shorter timeETH Zürich, Switzerland

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In-Depth Characterization of Staurosporine Induced Proteome Thermal Stability Changes, , , 2020Deep CETSA MS profiling of StaurosporinePelago Biosciences, Sweden

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cetsa dda offline-fractionation tmtthermo-q-exactive-hf
Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles, , 2020In vivo study of biocompatibility of magnetic nanoparticles. Toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined in vivo by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC−MS/MS.University of Southern Denmark

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dda lung tissue60-spdthermo-orbitrap-fusion-lumos
A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho.Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark, ,

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automation dda dia faims offline-fractionation phosphoproteomics ptm technology tissue tmt100-spd 200-spd 60-spdthermo-orbitrap-exploris-480
Covalent Protein Painting Reveals Structural Changes In The Proteome In Alzheimer Disease, , , 2020Development of Covalent Protein Painting (CPP), a structural proteomics approach to quantify changes in protein fold or altered protein-protein interaction for any protein in a proteome. CPP directly determines the relative surface accessibility of amino acid side chains by measuring the molar fraction of a chemical functionality that is accessible for chemical modification on the …Scripps Research Institute, United States

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alzheimer-disease brain dda tissue30-spdthermo-orbitrap-fusion-lumos
Advanced Host Cell Protein (Hcp) Analysis With Tims Qtof Ms Powers Biopharmaceutical Development , , 2019Descriptive piece of HCP analysis on the timsTOFBruker Daltonics, Germany

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antibodies dda hcp60-spdbruker-timstof-pro
Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry, 2019“Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases.Erasmus MC, The Netherlands

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bacteria targeted-workflow100-spdthermo-q-exactive-hf
Study: Increased De Novo Protein Sequencing Coverage With Optimal Protease Cocktail, 2019Increased De Novo Protein Sequencing Coverage with Optimal Protease CocktailRapid Novor, Canada

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antibodies ddathermo-orbitrap-fusion
High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition, , 2018High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway.Thermo Fisher Scientific, United States, ,

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phosphorylation ptm targeted-workflow100-spd 200-spd 60-spdthermo-q-exactive-hf-x
Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.Le Laboratoire des Courses Hippiques (LCH), France

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plasma rhuepo targeted-workflow100-spdthermo-q-exactive-hf