columns and emitters

Evosep columns and emitters deliver sharp symmetrical peaks robustly and reproducibly over thousands of runs.

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CHROMATOGRAPHIC EXCELLENCE WITH MATCHED COLUMNS

Standardized methods benefit from matched columns for the best chromatography and highest reproducibility.

Evosep offers two lines of consumables – Endurance for robust everyday use and Performance for exceptional results.

The recommended column for each method is listed here.

The Best chromatography, every time, all the time

The Endurance line is used for our standard methods for robust, reproducible performance at ambient temperature everywhere, for everyone, every time. This product line consists of EV1064 for 60/100 SPD, EV1106 for 30 SPD and EV1107 for 200/300/500 SPD.

ENHANCED PROTEOME COVERAGE WITH PERFORMANCE COLUMNS

Our line of Performance columns is for exceptional results, but also puts some demands on usage such as requiring elevated column temperature control to manage system backpressure (This flyer provides some recommendations to ensure the recommended 40°C)

The product line consists of EV1109 for 60 and 100 SPD methods and EV1137 for 30 SPD and the Extended method. These columns are packed with 1.5 μM C18 beads, which improve peak capacity significantly compared to the Endurance line of columns. Consequently, the proteome coverage is enhanced, and the column provides a robust and competitive option – on the same level as the home-pulled and packed options, but with better reproducibility and life-time.

EMITTERS AND OPEN-ENDED COLUMNS

Evosep emitters are designed with either stainless steel or fused silica and come with integrated liquid junctions for easy zero dead volume, finger tight connections in our source adapters.

Emitters are available in different materials and dimensions. The default is an ID 30 µm stainless steel emitter (EV1086), with an alternative ID 20 µm fused silica emitter (EV1087). The ID 10 µm fused silica emitter (EV1111) is exclusively for our 100 nl/min Whisper™ methods!

All our columns come default with fittings for our own emitters and spray adapters, but when those are not appropriate, you can find open-ended versions (marked OE) of our columns for connecting to your own spray solutions.

Learn more about our range of columns and emitters in our shop.