ApplicationsGet inspired with research from our users around the world and explore some of the many published applications with Evosep One
Understanding the COVID-19 disease using rapid assays with high sensitivity
The global emergence of novel coronavirus disease 2019 (COVID-19) has underlined the requirement for rapid assays with high sensitivity and specificity for detection of infection and further to understand the molecular functions complexity of the disease.
Explore how, our customers have used the Evosep One to develop an effective screening assay and comprehensive understanding of the disease.
REPRODUCIBLE PLASMA PERFORMANCE
Retention time stability of selected peptides over 96 runs
Pearson correlation matrix comparing the 96 runs
Low carry-over between runs (<0.1%)
Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers. Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.
PROTEIN IDENTIFICATION WITH SHORT GRADIENTS
Stable protein and peptide ID rates over 96 injections with the 200 samples/day methods
- Examples of identification rates possible with different methods.
All measurements were performed on a Bruker Daltonics timsTOF Pro with 50 ng of HeLa digest and at least 15 replicates.
Data courtesy: LCMS-141 app note, Bruker Daltonik GmbH, Germany
selected publications using short gradients
FAST GENERATION OF DIA LIBRARIES
Spectral library of mammalian cell line proteomes made from 46 fractions of HeLa digests.
Example ID and quantitation performance with 60 samples/day method
Selected publications with DIA libraries
Large clinical proteomics sample set
Enables large cohort studies in a fast and robust manner with
- Stable retention times
- High data consistency
across hundreds of injections
Selected publications on Large clinical proteomics sample sets
FAST AND SENSITIVE HCP SCREENING
- Bottom-up mass spectrometry protocols are established for analyzing host cell proteins (HCPs)
- Detecting low abundance HCPs confidently and with high throughput is now possible with the Evosep One paired with a trapped ion mobility separation TOF instrument
61 HCPs identified in 21 minutes from 25 µg NISTmAb (according to Huang et al., Anal. Chem. 2017, 89, 5436-5444) equivalent to approximately 1 µg load.
- 3 times faster*
- 2 times more sensitive*
*than 1h industry standard
Data courtesy: FN-06 flash note; Bruker Daltonik GmbH, Germany
SELECTED PUBLICATIONS on hcp analysis
Advanced Host Cell Protein (HCP) Analysis with TIMS QTOF MS Powers Biopharmaceutical Development
Rapid Host Cell Protein (HCP) Detection with Advanced Trapped Ion Mobility Spectrometry (TIMS) Technology
find all publications, product brochures and presentations with Evosep One
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