Get inspired with research from our users around the world and explore some of the many published applications with Evosep One

Understanding the COVID-19 disease using rapid assays with high sensitivity

The global emergence of novel coronavirus disease 2019 (COVID-19) has underlined the requirement for rapid assays with high sensitivity and specificity for detection of infection and further to understand the molecular functions complexity of the disease.

Explore how, our customers have used the Evosep One to develop an effective screening assay and comprehensive understanding of the disease.


Retention time stability of selected peptides over 96 runs

Pearson correlation matrix comparing the 96 runs

Low carry-over between runs (<0.1%)

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers. Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.


  • Stable protein and peptide ID rates over 96 injections with the 200 samples/day methods

  • Examples of identification rates possible with different methods.

All measurements were performed on a Bruker Daltonics timsTOF Pro with 50 ng of HeLa digest and at least 15 replicates.

selected publications using short gradients

A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients

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Spectral library of mammalian cell line proteomes made from 46 fractions of HeLa digests.

Example ID and quantitation performance with 60 samples/day method

Selected publications with DIA libraries

Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

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Large clinical proteomics sample set

Enables large cohort studies in a fast and robust manner with

  • Stable retention times
  • High data consistency

across hundreds of injections

Selected publications on Large clinical proteomics sample sets

Plasma proteomics goes high throughput –
timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min

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  • Bottom-up mass spectrometry protocols are established for analyzing host cell proteins (HCPs)
  • Detecting low abundance HCPs confidently and with high throughput is now possible with the Evosep One paired with a trapped ion mobility separation TOF instrument

61 HCPs identified in 21 minutes from 25 µg NISTmAb (according to Huang et al., Anal. Chem. 2017, 89, 5436-5444) equivalent to approximately 1 µg load.

  • 3 times faster*
  • 2 times more sensitive*

*than 1h industry standard


Advanced Host Cell Protein (HCP) Analysis with TIMS QTOF MS Powers Biopharmaceutical Development

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Rapid Host Cell Protein (HCP) Detection with Advanced Trapped Ion Mobility Spectrometry (TIMS) Technology

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Flash note on high-througput HCP Screening using the timsTOF Pro coupled with the Evosep One to detect HCPs with a 21 minute gradient. 

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