Plasma proteomics

Plasma proteomics powered by LC-MS is a valuable tool to support personalized healthcare by uncovering novel biomarkers and advancing our understanding of disease biology.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

Plasma proteomics

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

Unlock the future with only a finger prick 

Proteomics methodologies are gaining traction, leading to the discovery of numerous disease-related biomarkers and potential drug targets. By analyzing the diverse and complex protein content in blood plasma, researchers can identify biomarkers crucial for early diagnosis, prognosis, and personalized treatment strategies.

Requiring only a finger prick of blood, plasma proteome profiling could easily be performed throughout the patient’s lifetime, from birth to the later years of life. Certainly, for a widespread adoption of proteomics in the clinic, the entire workflow from sample preparation through LC-MS and data analysis needs to become significantly faster and more sensitive to enable the required throughput.

This approach offers a shift from conventional one-size-fits-all healthcare towards tailored interventions for individual patients. 

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One of the key advantages of plasma proteomics is its ability to detect proteins and post-translational modifications with high sensitivity and specificity.

However, plasma proteomics comes with its set of challenges. Plasma is a complex mixture of proteins, including low abundant proteins and those masked by more abundant ones. Additionally, some proteins may have modified sequences, further complicating their detection. Overcoming these challenges requires robust analytical techniques and optimized workflows to ensure reliable and reproducible results.

Mass spectrometry based proteomics enables researchers to quantify proteins and characterize protein patterns, offering a more comprehensive understanding of disease mechanisms and patient-specific responses to treatment. With the right technology researchers can overcome challenges and accelerate the translation of plasma proteomics research into new applications, ultimately improving patient outcomes.

Elevate efficiency with automation

Simplification and standardization of workflows is preferred for applications such as plasma proteomics.

The Evosep One not only facilitates robust and reproducible high-throughput analysis of large-scale sample cohorts but is also complemented by automated Evotip loading. The Evotip speeds up your sample loading, reduces carry-over and is a significant contribution to why proteomics has become so easy and routine.

This means that you can reduce manual labor, enhance efficiency, sensitivity, and cost-effectiveness, while ensuring consistent and reliable results across diverse sample cohorts. All ideal when doing large scale plasme proteomics.

Explore more about automated Evotip loading


scaling up Lung Cancer Screening with a multi system setup

In this study blood from over 2,500 patients were screened using multi-omics approaches for detection of proteins, metabolites, and transcripts with the aim of generating multiplexed biomarker panel for early lung cancer detection.

Due to high system to system reproducibility combined with our 60SPD standard method they were able to split Analysis across four Evosep One systems for parallel sample processing. This allowed for efficient screening of a large cohort of samples.

This Deep plasma proteome profiling was enabled by automated sample processing, based on the Seer Proteograph assay, coupled to the Evosep One and Bruker timsTOF HT mass spectrometers.

The proteome profiling workflow resulted in the detection of >8,000 protein groups in at least one subject and >5,900 protein groups detected in least 25% of the subjects.

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Center for Translational Biomedical Research at the University of North Carolina

Streamlining a high-throughput plasma proteomics platform with the 60 SPD method

In this study researchers from the Center for Translational Biomedical Research at the University of North Carolina have used the Evosep One combined with the 60SPD method to develop of a high-throughput, robust, and reproducible proteomics platform (sHTPP).

Through a subsequent 300-sample clinical study, their Evotip based workflow demonstrated high sample throughput with minimal interplate variability, indicating its robustness and reliability. Moreover, the sHTPP platform facilitated comprehensive analysis of plasma proteins in clinical samples, boasting high quantitative reproducibility and enabling confident comparison between samples.

The 60SPD method offers an excellent balance of throughput and depth making it a great fit for plasma proteomics.

Read full publication here

University of Oxford

Comprehensive and reliable profiling of the plasma proteome in sepsis patients with the 100 SPD method

By leveraging tandem mass spectrometry with high-throughput automated methods, the University of Oxford has successfully characterized the plasma proteome of over 1000 adult sepsis patients, integrating leukocyte transcriptomics to advance understanding of sepsis pathophysiology and facilitate the discovery of potential therapeutic targets and biomarkers.

In their study, a high-throughput quantitative proteomics workflow was developed using Evosep One combined with the Bruker timsTOF Pro,  analysisng more than 2600 plasma samples from 1600 individuals in a single batch, significantly increasing the throughput of MS-based proteomics.

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Boston Children’s Hospital

A cost efficient strategy for high-thorughput plasma depletion of >3000 samples

In this study researchers from the Boston Children’s Hospital present a simple, validated, time- and cost-efficient, high-throughput protocol for plasma depletion. Their method was used to process over 3200 plasma samples, proving to be both robust and highly reproducible. By leveraging the Evosep One system, the researchers streamlined and simplified their methodology, achieving notable cost savings.

The Evosep One system contributed to reduced consumable costs by maximizing sample recovery and minimizing sample loss, thereby decreasing the consumption of expensive chromatography materials and reagents. Overall, the study presents a cost-efficient and reliable high-throughput plasma depletion technique, facilitating comprehensive plasma profiling and detecting over 1300 proteins per run.

With a throughput of 60 samples per day, the method underwent extensive validation, processing over 3000 samples without any discernible batch effect, and costing approximately $2.5 per sample (reagents and lab ware) for the depletion step.

Read full publication here

Novo Nordisk Foundation of Center for protein research

Fully automated Evotip loading of up to 192 samples in six hours

This study address the challenges of doing high-throughput sample preparation in large cohort plasma studies without compromising sensitivity or reproducibility, even at low sample amounts.

To do so, they developed a Fully automated workflow for integrated sample digestion and Evotip loading. The workflow is based on the Opentrons OT-2 platform and combines PAC digestion using magnetic micro-beads with automated loading of resulting peptides directly on Evotips for reproducible desalting and storage of samples. By integrating digestion and sample loading on Evotips, it minimizes hands-on time, increases throughput, and significantly reduces sample input and required reagents.

Their results showcased that the workflow is reproducible and stable with a proteome depth on par with what would be expected in manual workflows.

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Scalable Plasma Proteomics with the Evosep One

Available on demand – hosted on APR 2024

The adoption of high-throughput techniques in plasma proteomics has transformed the discipline by enabling the simultaneous analysis of large patient cohorts and the identification of hundreds to thousands of proteins. This strategy has facilitated the uncovering of new biomarkers and therapeutic targets that had not been identified before.



Available on demand – hosted on JUN 2021

In this webinar you will learn how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

New application note

Unlocking the plasma proteome with Evotip Pure based, standardized and scalable workflows

Plasma stands out as one of the most readily accessible biofluids, with an estimated presence of over 10,000 distinct proteins. A significant portion of these are released from organs and tissues. The ability to measure such proteins in a robust manner holds tremendous potential for advancing next-generation multiplexed biomarker discovery.


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find all publications, product brochures and presentations with Evosep One


Application Note
Evotip Pure brings innovative sample storage
Application Note
Unlocking the plasma proteome with Evotip pure based, standardized and scalable workflows
Scalability for high-throughput proteomics –
Evotip Pure integration with the Biomek i5 liquid handler
columns and emitters overview
application note
Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
application note
end-to-end, fully automated digestion protocol and evotip pure workflow on the opentrons ot-2
application note
fully automated, rapid, robust sample loading on evotip pure with the agilent assaymap bravo
EVOSEP ONE: A Standardized Separation
Tool for Clinical Omics

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