HIGH-THROUGHPUT PLASMA ANALYSIS

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

HIGH-THROUGHPUT PLASMA ANALYSIS

Proteomics analysis of human blood offers an attractive and challenging pipeline for discovery of biomarkers.

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

LESS SAMPLE PREP WORKFLOW STEPS

Simplification of workflows are preferred for standardized solutions. The Evotip serves as an integrated purification step, which saves time compared to conventional methods as some time-consuming steps can be omitted, when using the Evosep One.

The procedure is compatible with all upstream sample preparation protocols and for large cohort studies, where samples are often prepared with automated sample processing robots, the 96-well format of the Evotips fits perfectly.

CLINICAL APPLICABILITY TO THE PLASMA PROTEOME

The Evosep One is designed for high throughput applications, with a focus on clinical analysis. Blood plasma is the most widely analyzed clinical matrix, with million of samples drawn daily.

Reproducibility is essential in clinical proteomics and across 96 independent, parallel sample preparations and injections of the same original plasma measured in less than two days of LC-MS time, the correlation over all runs is excellent.

See the results and read more here

PLASMA PROTEOMICS GOES HIGH-THROUGHPUT WITH 100 SAMPLES PER DAY

In collaboration with Roman Fischer, University of Oxford and Bruker Daltonik,  we combined the robustness and speed of the timsTOF Pro with PASEF together with the high throughput LC-separation provided by the Evosep One to analyze 100 ng samples of blood plasma from 192 severe infection patients at a sampling rate of 100 samples per day.

It provides robust quantification of more than 500 proteins in a single run and 772 collectively quantified if all runs are merged together.

See the results and read full app note here.

STANDARDIZED WORKFLOWS FOR LARGE COHORT PLASMA PROFILING

Thermo Fisher Scientific and Evosep are on a joint mission to develop standardized and scalable plasma protein quantitative profiling workflows. Our recent developments have been featured in an application note by the Precision Medicine Science Centre led by Dr. Emily Chen.

The aim of the study was to develop a standardized, high throughput, and reproducible workflow for bloodbased biomarker discovery using automated sample preparation. The 60 samples per day method was chosen for the study. The results demonstrated 3% CV for retention time shift over hundreds of repeated measurements and <15% CV for peak area quantification while maintaining the sensitivity.

See the results and read full app note here.

EVOSEP WEBINAR

MEET OUR USERS IN PLASMA RESEARCH

EVOSEP WEBINAR: Plasma proteomics WITH EVOSEP ONE

JUN 24  I  4:00 pm CET  /  10:00 am EDT

In this webinar you will learn how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

THE FUTURE OF BLOOD TESTING

Mass spectrometry-based proteomics has become a powerful technology in biological research, emphasizing the need for robust, fast and efficient sample preparation.

Read the full overview of clinical plasma proteomics here.

Standardized and robust workflows are essential for quantitative protein profiling in large sample cohorts. With pre-set gradients, the Evosep One is easy to operate, while maintaining reproducibility and sensitivity in a high-throughput manner.

Emily Chen, Sr. Director,
Thermo Fisher Precision Medicine Science Center

 

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find all publications, product brochures and presentations with Evosep One

More research on plasma

Here you can see publications available on plasma research featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids, , 2021The Van Eyk group presents a standardized workflow for precise high-throughput proteomics of blood biofluids using the 60 SPD method. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood.

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The COVIDome Explorer Researcher Portal, , , 2021Researchers from University of Colorado led by Joaquin M. Espinosa, have created a user-friendly researcher portal enabling easy access and real-time analysis of matched multi-omics datasets for COVID-19, termed the COVIDome Explorer. They illustrate the use through a multi-omics analysis of biosignatures associated with C-reactive protein (CRP), an established marker of poor prognosis in COVID-19.

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A robust mass spectrometer for precision medicine – the Orbitrap Exploris 240 mass spectrometer for large-scale plasma protein profiling, 2021This application note from Thermo Fisher Scientific Precision Medicine Science Center demonstrates the reliability and robustness of the Evosep One coupled with the Orbitrap Exploris 240 MS for large-scale, untargeted plasma protein profiling. They observed excellent reproducibility of protein and peptide identifications over 100 injections of human serum performed five weeks apart.

Download application note

Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers, , , 2021In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker.,

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Seroconversion stages COVID19 into distinct pathophysiological states, , 2020In this study, researchers from University of Colorado led by Joaquin M. Espinosa, found highly variable seroconversion status among hospitalized Covid-19 patients. Their results support the existence of distinct pathophysiological states, with seroconversion status being potentially useful as a surrogate marker of underlying processes.

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Gradient off-set focusing HPLC insturment for robust and high throughput clinical proteomics, , 2017Evosep One for clinical analysis

Download application note

A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics, , , 2018Description of Evosep

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Robust and Reprodusible Protein Quantification in Plasma Using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS, , 2020Agilent feasibility study

Download application note

High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIA,

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Clinical proteomics, 2018Clinical proteomics poster

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Development of a Novel LC Concept for Clinical Proteomics, , 2018Description of Evosep One

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MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysis

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Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).

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Consistency, consistency – Automated Sample Prep for Translational Proteomics, 2020In this webinar, Emily Chen, Sr. Director at the Thermo Fisher Precision Medicine Science Center presents an automated, robust and scalable sample preparation pipeline for large-scale clinical research samples.

Watch webinar

An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer, , , , , 2020In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.,

Download poster or booklet

Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard., ,

Learn more or download application note

A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, , , 2020This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …

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Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.

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