Evotip Pure can bind approximately 1µg of pure peptides. However, presence of different organic molecules in the sample can reduce the binding capacity through competition, and the optimal sample load may also be dependent on other workflow components.
Evosep recommend following the Evotip loading protocol exactly and loading 20µl of sample. We have tested and were unable to detect losses from diluting a low volume sample to 20µL for loading on Evotip Pure. If the user chooses to change the volume the loading...
No, they are a single use consumable. Once the sample has been eluted from the Evotip, the plastic will be slightly deformed and cannot be used again. Doing so risks instrument damage and will void instrument warranty.
Only Evotip Pure are compatible, tested and validated for use with the Evosep Eno. Evotip Pure are designed for a perfect low-pressure, leak free fit with the Evosep Eno. Use of any other product for sample introduction is not permitted and will void instrument...
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