whisper Zoom 120 SPd

Specialized method

Pushing boundaries for scalability with high sensitivity

The Whisper Zoom 120 SPD method is designed to push the boundaries of scalability for high-sensitivity applications.

Uncover single cell heterogeneity with the throughput and robustness needed.

EASE-OF-USE AND ROBUSTNESS DRIVES EFFORTLESS THROUGHPUT

The Whisper Zoom 120 SPD method is possible through a highly optimized flow path, reducing physical constraints to a bare minimum for maximized sample recovery and performance.

While the throughput is needed to process large single cell cohorts, robustness is essential to actually make use of the potential. The Evosep One is robust by design, leveraging Evotip technology to enhance the column longevity through partial elution and single-use trap columns.

To demonstrate the stability, we monitored the pump pressure profile across 340 samples and the constant pressure throughout the analysis illustrates that the analytical column is well-protected by the partial elution enabled by the Evotip Pure.

Whisper zoom pushes boundaries for robust and scalable single cell proteomics

Recent improvements in single cell proteomics (SCP) have shown the most pronounced leap in performance in recent years. The developments of new technologies such as loss-less sample preparation workflows and modern high-end mass spectrometers has been a community effort. Currently, the link between sample preparation and MS detection is still a challenge due to sample losses and efficient high performing separation.

The Whisper Zoom methods are specifically designed to significantly improve and maximize recovery of the sample to deliver effortless sensitivity and robustness with unmatched performance. This combined with the easy-to-use and robust Evosep technology have commoditized high-end-LC-MS performance and made it available to all types of research areas.

EVOTIP BRINGS INNOVATIVE SAMPLE STORAGE INTRODUCTION TO HIGH-SENSITIVITY LC-MS WORKFLOWS

The Evotip, a disposable trap column, is the core of our technology. The Evotip serves as an integrated purification step, where contaminants are left on the tip, and analytically relevant peptides are eluted.

The Evotip is extraordinary important for ultra-sensitive analysis, as very small sample amounts can be loaded in higher volumes.

Furthermore, loaded Evotips can be stored cold until analysis, which is especially important for single cell proteomics, where very limited sample amount sets higher demands to the storage of peptide and become a crucial factor for reproducible analysis.

Explore the evotip

EFFICIENT UTILIZATION OF PEPTIDE ELUTION GIVES YOU HIGH PERFORMANCE

The Evosep One employs standardized methods with fixed columns. By employing these specifications, you don’t need to spend time optimizing the methods. You achieve hazzle-free high performance every time.

Whisper Zoom 120 SPD is optimized for speed and delivers efficient peptide elution over ~6 minutes. Chromatographic peaks are sharp with average median FWHM of ~1.6 seconds.

Get all the details about the method and learn how to set it up correctly.

INNOVATIVE SAMPLE CAPTURE AND RECOVERY ALLOWS FOR SENSITIVE ANALYSIS

In contrast to other omics technologies, a prerequisite for proteomics is to get the best results possible from the starting material available. This sets high requirements to minimizing losses – a challenge throughout complex sample preparation workflows as each sample transfer step entails a source of variation and sample loss through peptide adsorption and loss of sample volume.
The Evotip reduces the number of sample transfer steps compared to alternative strategies, but still benefits from using n-dodecyl-β-D-maltoside (DDM) for low sample loads until saturation is observed at higher loads. As peptides are stored equally well on the Evotip regardless of using DDM, the beneficial effect from DDM impacts the sample preparation workflow upstream of the Evotip.

Read more about the results in the application note.

Improved Whisper™ methods drive scalable workflows

Available on demand – hosted on may 2024

In this webinar you can meet Evosep One user Christoph Krisp, Application Development Specialist Proteomics at Bruker Daltonics and learn about his latest research regarding high sensitivity proteomics in scalable single cells on the timsTOF Ultra using our new Whisper Zoom methods.

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explore all specialized applications available

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Application Note

A specialized method for high sensitivity with the Whisper Zoom 120 samples per day method

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Application Note

Whisper Zoom drives scalable workflows

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Whisper Zoom

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whisper Zoom 120 SPd

Specialized method

Pushing boundaries for scalability with high sensitivity

EASE-OF-USE AND ROBUSTNESS DRIVES EFFORTLESS THROUGHPUT

Whisper zoom pushes boundaries for robust and scalable single cell proteomics

EVOTIP BRINGS INNOVATIVE SAMPLE STORAGE INTRODUCTION TO HIGH-SENSITIVITY LC-MS WORKFLOWS

EFFICIENT UTILIZATION OF PEPTIDE ELUTION GIVES YOU HIGH PERFORMANCE

INNOVATIVE SAMPLE CAPTURE AND RECOVERY ALLOWS FOR SENSITIVE ANALYSIS

Improved Whisper™ methods drive scalable workflows

Available on demand – hosted on may 2024

explore all specialized applications available