applications to a
EVOSEP+ ADDS SPECIALIZED APPLICATIONS TO A STANDARDIZED PLATFORM
Here we introduce a new set of methods that extend the potential of the Evosep One platform for clinical research. While expanding our scope for specialized methods, we continue to deliver these with our key values such as ease of use, speed, robustness and sensitivity.
EVOSEP+ is a dynamic environment, which continuously provides simplified solutions to new applications.
robust, very low nanoflow single-cell analysis with the WHISPER 40SPD BETA method
The Whisper 40SPD beta method is ideal for ultra-sensitive applications, such as single cell proteomics, where one of the main challenges is to maintain sensitivity and robustness throughout the workflow.
ULTRA-SENSITIVE ROUTINE APPLICATIONS with the WHISPER 20 SPD BETA method
The Whisper 20SPD beta method features a longer gradient in a series of methods developed with Whisper Flow Technology.
It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.
MORE PROTEOME COVERAGE IN A SINGLE EXPERIMENT WITH THE EXTENDED METHOD
This Extended Evosep One method is designed for maximum proteome coverage in a single-shot analysis with an 88 min gradient corresponding to a throughput of 15 samples per day.
The raw identifications allow for 35,000 unique peptides resulting in 5000 proteins from data-dependent acquisition with excellent reproducibility.
See the results and read more here.
Data courtesy: Professor Matthias Mann and Johannes Müller, Max-Planck Institute of Biochemistry, Proteomics and Signal Transduction on a Thermo Scientific Orbitrap Exploris 480 MS
Robust and high-throughput analysis with the Lipidomics method
Lipidomics is usually challenged by difficult storage conditions of volatile lipid extracts. This is solved by loading and storing the extracts on Evotips.
We observe excellent stability of lipid extracts on the Evotip for up to 30 h, which is essential for the scalability of the workflow. With a throughput of 60 samples per day, we identify more than 1000 lipids from only 1 μL of human plasma.
This method is still in development. Preliminary results will be shared soon.
LARGE SCALE DOPING ANALYSIS WITH THE HIGH ORGANIC METHOD
The High Organic method is specifically developed for targeted analysis of stanozolol in equine urine and hair.
It enables small-molecules analysis of significantly more hydrophobic targets. Thus, the analytes are sequentially eluted of the Evotips with up to 100% acetronitrile. The method has a throughput of 100 samples per day and is designed for our EV1064 column.
“With a key focus on robustness, speed and standardization, we are proud to introduce EVOSEP+ as a solution for specialized applications for MS-based omics.”,
Nicolai Bache, Head of Applications at Evosep
explore all available methods with evosep+
Find the method fitting your research need and get inspired with data from Evosep users.
The extended method
Maximum proteome coverage in a single-shot analysis with extended gradient duration.
The High organic method
This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.
The ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell analysis.
The lipidomics method
DOWNLOAD OUR MATERIAL
GRADIENT OFF-SET FOCUSING HPLC
INSTRUMENT FOR ROBUST AND HIGH
THROUGHPUT CLINICAL PROTEOMICS
We’d love to hear from you!
Need more information, do not hesitate to contact us.