EVOSEP+

Adding specialized

applications to a

standardized platform

EVOSEP+ ADDS SPECIALIZED APPLICATIONS TO A STANDARDIZED PLATFORM

Here we introduce a new set of methods that extend the potential of the Evosep One platform for clinical research. While expanding our scope for specialized methods, we continue to deliver these with our key values such as ease of use, speed, robustness and sensitivity.

EVOSEP+ is a dynamic environment, which continuously provides simplified solutions to new applications.

 

robust, very low nanoflow single-cell analysis with the WHISPER 40SPD BETA method

The Whisper 40SPD beta method is ideal for ultra-sensitive applications, such as single cell proteomics, where one of the main challenges is to maintain sensitivity and robustness throughout the workflow.

See the results and learn more 

ULTRA-SENSITIVE ROUTINE APPLICATIONS with the WHISPER 20 SPD BETA method

The Whisper 20SPD beta method features a longer gradient in a series of methods developed with Whisper Flow Technology.

It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.

See the results and learn more 

MORE PROTEOME COVERAGE IN A SINGLE EXPERIMENT WITH THE EXTENDED METHOD

This Extended Evosep One method is designed for maximum proteome coverage in a single-shot analysis with an 88 min gradient corresponding to a throughput of 15 samples per day.

The raw identifications allow for 35,000 unique peptides resulting in 5000 proteins from data-dependent acquisition with excellent reproducibility.

See the results and read more here.

Data courtesy: Professor Matthias Mann and Johannes Müller, Max-Planck Institute of Biochemistry, Proteomics and Signal Transduction on a Thermo Scientific Orbitrap Exploris 480 MS

Average protein identifications from five technical replicates with the Extended method.

Robust and high-throughput analysis with the Lipidomics method

Lipidomics is usually challenged by difficult storage conditions of volatile lipid extracts. This is solved by loading and storing the extracts on Evotips.

We observe excellent stability of lipid extracts on the Evotip for up to 30 h, which is essential for the scalability of the workflow. With a throughput of 60 samples per day, we identify  more than 1000 lipids from only 1 μL of human plasma.

This method is still in development. Preliminary results will be shared soon.

“With a key focus on robustness, speed and standardization, we are proud to introduce EVOSEP+ as a solution for specialized applications for MS-based omics.”,

Nicolai Bache, Head of Applications at Evosep 

 

explore all available methods with evosep+

 

Find the method fitting your research need and get inspired with data from Evosep users.

The extended method 

EVOSEP+ method

Maximum proteome coverage in a single-shot analysis with extended gradient duration.

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Whisper
40SPD beta

EVOSEP+ method

The ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell analysis.

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Whisper
20SPD beta

EVOSEP+ method

A longer method for sensitive nanoflow applications.

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Whisper™ 10 SPD

EVOSEP+ method

Coming soon

The lipidomics method

EVOSEP+ method

In development

We’d love to hear from you!

Need more information, do not hesitate to contact us.