Quantitative accuracy in proteomics
Whether you’re exploring the proteome with broad DIA coverage, pinpointing specific targets with PRM/MRM, or using DDA for discovery and spectral library generation, your ability to trust the data relies on how well you can quantify it.
Explore how Evosep delivers robust, reproducible results across all workflows.
Next generation LC-MS based proteomics require high quantitative accuracy
Proteomics is no longer just about how many proteins you can identify but increasingly about how confidently and consistently you can quantify them. As the field shifts toward real-world applications like biomarker validation, clinical assays, and regulated workflows, quantitative accuracy has become a must have.
This transition demands workflows that are not only sensitive, but also standardized, scalable, and reproducible. Evosep enables this shift by delivering robust LC-MS performance across targeted proteomics, DIA, and DDA workflows.
Read our latest blog post to explore how Evosep is bridging the gap between golden runs and biological insights.
Targeted Proteomics
Targeted analysis offers precise quantification of selected proteins, making it indispensable for biomarker validation, clinical assays, and regulated workflows.
Its strength lies in delivering low variability and high confidence in known targets, especially in complex matrices like plasma or bioprocess samples.
data independent acquisition
DIA provides comprehensive and consistent coverage of the proteome, capturing fragment ion data for all detectable peptides.
This approach is ideal for large-scale studies where data completeness and reproducibility are key.
data dependent acquisition
DDA enables deep proteome profiling and supports the development of hybrid workflows that enhance DIA performance.
When paired with our standardized methods, DDA delivers consistent and scalable results.
The Cornerstone of meaningful proteomics
The ability to quantify proteins with precision, reproducibility, and scalability is essential for both discovery and translational research.
Within this type of workflows you can find targeted proteomics, data independent acquisition and data dependent acquisition. Each method contributes uniquely to achieving high-quality quantitative results. While their analytical goals may differ, they share a common demand for reproducibility, sensitivity, and scalability.
Together they form a comprehensive toolkit for researchers aiming to extract reliable quantitative insights from complex biological samples.
Evosep webinar
Targeted Workflows for Confident Quantification
September 25th 2025 at 4:00 PM CEST / 10:00 AM EDT
Push the boundaries of targeted proteomic LC-MS workflows designed for unmatched sensitivity, reproducibility, and throughput. See how streamlined methods enable confident protein quantification and accelerate validation across large cohorts with ease.
Highly reproducible data with standardized methods
Low analytical variability is essential when analyzing large cohorts, validating biomarkers, or generating spectral libraries. Across all acquisition strategies Evosep ensures reproducibility through our standardized methods.
Calibrated flow control and matched columns deliver consistent retention times and peak shapes, minimizing technical variation. This precision is especially critical in targeted workflows, where reproducibility directly impacts quantitation accuracy; in DIA, where consistent sampling across runs enables robust statistical comparisons; and in DDA, where reliable peptide identification supports high-quality library generation.
From sample preparation to acquisition and data analysis, Evosep’s streamlined workflows support short gradients and advanced software solutions, enabling confident quantification across diverse proteomics applications.
balancing sensitivity, reproducibility, and throughput
Targeted plasma proteomics with Evosep Eno
In this application note, we showcase a targeted proteomics workflow using PRM on an Orbitrap platform, optimized for low variability and high precision across replicates. Combined with Evosep Eno’s standardized gradients and matched columns, we achieved consistent retention times and robust peak shapes, even in complex plasma matrices.
This workflow was designed to be scalable for large cohorts, with minimal sample preparation variability and streamlined data analysis. This makes it ideal for biomarker validation and clinical research where reproducibility is essential.
Targeted proteomics
Extending the toolkit for targeted proteomcis
At the Technical University of Copenhagen researchers are working to overcome previous limitations in targeted proteomics, particularly around sensitivity for low-abundance proteins and throughput in complex samples.
Using SureQuant standard assays they can now process up to 100 samples per day on their Evosep instrument while maintaining robustness and quantifying proteins spanning six orders of magnitude.
The workflow delivered robust and reproducible results, with consistent sensitivity for both high- and low-abundance proteins, setting a new benchmark for clinical and large-scale biomarker studies.
ACCELERATED DIA-PASEF WITH DIA-NN
The collaboration between the Ralser group at the Crick Institute, UK and the Nesvizhskii group at University of Michigan, USA introduce the latest addition to the DIA-NN software, which was originally conceived to maximize the performance of fast proteomics experiments. They reprocessed the dia-PASEF reference dataset (read more about dia-PASEF with Evosep One here), where 200 ng of HeLa peptides were analyzed in triplicates with the 60, 100 and 200 samples per day methods.
By using a spectral library, DIA-NN quantified on average 5056 proteins from 200ng HeLa digest analyzed with the ultra fast 200 SPD Evosep One method. Notably, they obtained very high data completeness of 94%. By extending the gradient time with the 100 and 60 SPD methods, the proteomic depth increased to 6104 and 6737 proteins respectively. The median CV were below 8% for all three methods.
Read publication here
EVOSEP WEBINAR
MEET OUR USERS and their research using DIA
Webinar I Targeted Workflows for Confident Quantification
Push the boundaries of targeted proteomic LC-MS workflows designed for unmatched sensitivity, reproducibility, and throughput. See how streamlined methods enable confident protein quantification and accelerate validation across large cohorts with ease.
Webinar I Revolutionizing proteomics with evosep dia
Unleash the power of DIA and enable the exploration of complex biological systems with unprecedented depth and precision for large-scale quantitative protein analysis combined with short methods on the Evosep One.
Webinar I Unleash the power of DIA
In this webinar we will dive into the exciting research possibilties that comes by using DIA combined with short gradients with Evosep One.
Webinar I Unleashing the power of dia combined with short gradients
In the ever lasting effort to boost the number of protein identified in MS-based proteomic experiments, it has become clear that Data Independent Analysis (DIA), combined with short gradients, has become a winning combination.
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Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
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fully automated, rapid, robust sample loading on evotip pure with the agilent assaymap bravo
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EVOSEP ONE: A Standardized Separation
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