EVOSEP Eno: EV1500
Evosep Eno is an easy-to-use, robust and reliable chromatographic solution for high performance and standardized proteomics, preparing for next generation workflow integration.Next generation insights with Evosep Eno
LC-MS based proteomics is not just about individual technologies. It covers the entire workflow from sample preparation to data acquisition – ensuring sensitivity, reliability, and speed.
The Evosep™ Eno is a standardized platform designed for robustness and high throughput, integrating sample preparation with LC-MS analysis to streamline and optimize your entire workflow – not just individual performance metrics.
Maximum reliability: Designed to keep your research on track with out-of-the-box productivity, providing close to 100% uptime.
Cost-efficient: An affordable solution lowering the cost-per-sample by 64% while increasing MS utilization 29%
Patented approach: Innovative sample introduction and separation technology for seamless, efficient operation (EP3519808B1, EP3519807B1, US12298284B)
Next generation insights with Evosep Eno
LC-MS based proteomics is not just about individual technologies. It covers the entire workflow from sample preparation to data acquisition – ensuring sensitivity, reliability, and speed.
The Evosep™ Eno is a standardized platform designed for robustness and high throughput, integrating sample preparation with LC-MS analysis to streamline and optimize your entire workflow – not just individual performance metrics.
Maximum reliability: Designed to keep your research on track with out-of-the-box productivity, providing close to 100% uptime.
Cost-efficient: An affordable solution lowering the cost-per-sample by 64% while increasing MS utilization 29%
Patented approach: Innovative sample introduction and separation technology for seamless, efficient operation (EP3519808B1, EP3519807B1, US12298284B)
Excellent just got better
Evosep Eno sets new standards for routine and high-performance LC-MS
LC-MS based proteomics has rapidly evolved from a specialized analytical discipline, into a powerful, applied technology to elucidate detailed biological insights at scale.
The Evosep Eno is our latest innovation to enable requirements for next generation LC-MS based proteomics through simplicity, reliability and efficiency. It is a game-changing technology, allowing for routine execution of up to 500 samples per day, with industry-leading robustness and reliable chromatographic performance for consistency across instruments and laboratories every single day.
CHROMATOGRAPHY MATTERS – BOOST YOUR LC-MS PERFORMANCE
Chromatography lays the foundation for good MS performance, and improvement in the chromatography is directly translated into improved MS performance.
The Evosep Eno deliver up to 40% gain in precursor coverage comparedto Evosep One, independent of downstream MS platform. The gain is mostly pronounced at that faster methods, where MS performance is pushed to the limits and thus becomes more sensitive to chromatographic performance. Altogether, standardized chromatographic improvements on the Evosep Eno gives you:
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Peak symmetry improved with up to 35%
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20% sharper peaks at base, leading to higher peak capacity
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Up to 80% better retention time reproducibility
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Significantly improved intensity and quantification
INNOVATION DESIGNED TO OVERCOME THE CHALLENGES IN LC-MS BASED PROTEOMICS
Technology developments have been driving the pace of proteomics, pushing the throughput to achieve comprehensive proteomes in less than an hour, enabling large-scale applications and routine workflows. The Evosep Eno is designed to meet exactly these needs.
The instrument is engineered and built for maximum reliability. By using robust, low-pressure pumps and components to withstand wear and tear from continuous sample elution and gradient formation, it is possible to ensure operation of over tens of thousands of samples.
You don’t have to interrupt your analyses to troubleshoot the instrument – you will have a system that is always ready, when you are.
INNOVATION DESIGNED TO OVERCOME THE CHALLENGES IN LC-MS BASED PROTEOMICS
Technology developments have been driving the pace of proteomics, pushing the throughput to achieve comprehensive proteomes in less than an hour, enabling large-scale applications and routine workflows. The Evosep One is designed to meet exactly these needs.
The instrument is engineered and built for maximum reliability. By using robust, low-pressure pumps and components to withstand wear and tear from continuous sample elution and gradient formation, it is possible to ensure operation of over tens of thousands of samples.
You don’t have to interrupt your analyses to troubleshoot the instrument – you will have a system that is always ready, when you are.
Hear from the Evosep Eno users
Next generation LC-MS based proteomics
– A new era of speed, scale and robustness
Hear from three early adopters share how the Evosep Eno prepares them for next-generation proteomics – the paradigm shift that will launch scalable and robust sample interrogation in application areas like systems biology and precision medicine. Hear from:
- Matthias Mann, Director of Proteomics and Signal Transduction at the Max Planck Institute
- Dr. Jesper V. Olsen, Executive Director of the Novo Nordisk Foundation Center for Protein Research
- Dr. Ulises Guzman, Postdoctoral Researcher in the Olsen Group
webinar I available on demand
Evosep Eno: A New Era of Speed, Scale and Robustness
Discover how Evosep Eno simplifies workflows and supports labs of all sizes.
The Evosep Eno represents a significant leap forward in proteomics technology — delivering the speed, precision, and reproducibility needed to transform drug discovery and translational research.
Short learning curve and ease-of-use
The versatility and ease-of-use of the Evosep Eno benefits any type of experiment, from multiple, smaller projects to the continuous analysis of large sample cohorts.
You will instantly become an expert on the instrument and achieve cutting-edge performance – simply follow the listed column and emitter specifications to setup the method to confirm performance and reproducibility using the individual method application notes.
Redefining LC-MS proteomics workflows
Evosep Eno is different than conventional LC systems for a reason. It integrates sample preparation with LC-MS in a standardized and seamless manner providing ultimate robustness.
The Evotip is the sample introduction device, and is key in this integration through efficient capture and recovery of the sample, providing immediate improvement in the sensitivity of the workflow.
How well does your current setup align with this redefined approach? Are you ready to move beyond individual components and adopt a solution that enhances efficiency, scalability, and performance across your entire proteomics workflow?
Short learning curve and ease-of-use
The versatility and ease-of-use of the Evosep Eno benefits any type of experiment, from multiple, smaller projects to the continuous analysis of large sample cohorts.
You will instantly become an expert on the instrument and achieve cutting-edge performance – simply follow the listed column and emitter specifications to setup the method to confirm performance and reproducibility using the individual method application notes.
Innovative sample introduction with the Evotip
The Evotip Pure is the heart of the Evosep technology. It is a precision technology device with extremely high efficiency, integrating desalting with LC-MS sample introduction.
Samples are efficiently concentrated and captured on the Evotip and desalted offline. Once the MS is available, you can simply transfer the box of loaded Evotips to the Evosep Eno without the need for additional preparation.
The Evosep Eno will elute all peptides, while contaminants and large hydrophobic molecules remain on the Evotip, which is disposed after each run. This partial elution concept extends the column lifetime, and reduces the amount of carryover, ultimately eliminating the need to use expensive MS time to analyze blank samples.
Innovative sample introduction with the Evotip
The Evotip Pure is the heart of the Evosep technology. It is a precision technology device with extremely high efficiency, integrating desalting with LC-MS sample introduction.
Samples are efficiently concentrated and captured on the Evotip and desalted offline. Once the MS is available, you can simply transfer the box of loaded Evotips to the Evosep Eno without the need for additional preparation.
The Evosep Eno will elute all peptides, while contaminants and large hydrophobic molecules remain on the Evotip, which is disposed after each run. This partial elution concept extends the column lifetime, and reduces the amount of carry-over, ultimately eliminating the need to use expensive MS time to analyze blank samples.
Sustainable and scalable workflows
The Evosep Eno is designed to meet the needs of large-scale LC-MS based proteomics. This is realized through robust instrument design leveraging parallel operation of sample elution and gradient delivery to the analytical column to ensure maximum MS utilization of up to 90%. This robust and continuous operation easily allows for the routine analysis of hundreds of samples daily.
As a result, the bottleneck in high-throughput proteomics workflows has shifted towards sample preparation. The demand for robust, high-throughput sample preparation necessitates standardized and fully automated end-to-end workflows. This can be achieved through miniaturized – yet scalable Evotip-based workflows ensuring a more cost-efficient and sustainable sample preparation.
A green transition for LC-MS based proteomics
The Evotip simplifies sample handling from digestion to separation, reducing the number of single-use plastic tips needed to load a single sample.
The Evotip allows the Evosep Eno to handle up to 500 samples per day, using 79% less solvent and 35% less plastic per sample compared to a traditional nLC. During sample preparation, the sample is loaded onto the Evotip, eliminating the need for extra consumables like vials, pipette tips, and solvents for elution and re-suspension.
Additionally, the minimal carryover of Evotips reduces or eliminates the necessity for blank runs during experiment setup, leading to significant savings in liquid organic waste and electricity usage.
Versatility through standardized methods
The Evosep Eno, with its six standard methods, supports a range of use cases from comprehensive proteome analysis to ultra high-throughput single-shot analysis.
It redefines consistency and efficiency in proteomics workflows with optimized methods and dedicated columns, ensuring reproducible performance and simplifying application support.
By minimizing overhead time, it maximizes mass spectrometer utilization, generating more data in less time. Focused on accelerating MS development, it empowers labs to push the boundaries of sensitivity, scalability, and throughput without compromise.
Whisper Zoom methods sets a new standard
Whisper Zoom delivers unparalleled sensitivity for highly versatile applications. These robust methods enable high-throughput analysis for inter-laboratory studies, with a short learning curve that facilitates rapid method adaptation.
This cutting-edge performance addresses the increasing need for scalability, ease-of-use, and robustness, allowing researchers to quickly adopt high-sensitivity workflows with minimal training.
By maximizing recovery and achieving superior chromatographic performance, Whisper Zoom sets a new standard in proteomics research.
On demand webinars
Discover Proteomics at your own pace
Explore our large collection of on-demand webinars, covering everything from beginner guides to advanced workflows with Evosep. In each webinar you can hear directly from our users as they share their research and experiences with their Evosep instrument.
Additional resources
Get to know Evosep and our technology through the experiences of those using it daily. Discover all publications our users and collaboration partners or dive into our many applications and tech notes.
DOWNLOAD OUR MATERIAL
case study
Next generation LC-MS based proteomcis – a new era of speed, scale and robustness
application note
Evosep Eno – new standards for routine, scalable and high-performance LC-MS based proteomics
tech note
Introducing Evosep Eno
application note
Reproducible, inter-laboratory, scalable and routine LC-MS based Proteomics – With Evosep Eno
application note
EVOTIP PURE FOR END-TO-END WORKFLOWS: A COST-EFFICIENT, SUSTAINABLE AND SCALABLE PROTEOMICS SOLUTION
application note
EVOTIP PURE BRINGS INNOVATIVE SAMPLE STORAGE AND INTRODUCTION TO HIGH-SENSITIVITY AND SCALABLE LC-MS WORKFLOWS
application note
EVOTIP PURE SIMPLIFIES WORKFLOWS WITH EXCELLENT REPRODUCIBILITY, STORAGE AND RECOVERY
brochure
EVOSEP ONE:
A Standardized Separation
Tool for proteomics
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