The ability to identify and quantify large number of proteins and peptides is of great importance in translational medicine and life science research. Data independent acquisition (DIA) approaches have been shown to surpass data dependent acquisition (DDA) methodologies in terms of protein identifications in complex matrices especially at shorter acquisition speeds. Our new QTOF system equipped with a novel Zeno trap is able to deliver sensitivity gains in variable window SWATH acquisition. The built-in Zeno trap increases duty cycle at MS/MS level to over 90%, allowing for unprecedented gains in sensitivity (5-20x) at MS/MS, resulting in more identifications using Zeno SWATH acquisition. We evaluated increases in protein and peptide identifications using Zeno SWATH vs. SWATH acquisitions at various sample throughputs.
FP 110 – Leveraging a Higher Duty Cycle DIA Acquisition On a Novel QTOF for Enhanced Proteomics Analysis
by Eva Bryderup