With increasing focus on large-scale mass spectrometry studies, especially in clinical proteomics, there is a growing need for robust and sensitive high-throughput methods. Recent technological advances, such as preformed gradients in the Evosep One LC system, allow for the analysis of up to three hundred samples per day. Although appealing for high-throughput applications, these short gradients provide less coverage of the proteome and necessitate shorter cycle times in data-independent acquisition (DIA) strategies. Although this can be accommodated by increasing spectral complexity (larger DIA windows) or lowering mass resolution, data quality is reduced. To counteract this, we implement the Phase-Constrained Spectrum Deconvolution Method (ΦSDM) for Orbitrap signal processing, increasing acquisition speed for improved spectra quality and protein identification in short gradients.
MP 039 – Rapid proteome analysis using DIA and super-resolution Orbitrap mass spectrometry
by Eva Bryderup