Recently, the development of mass spectrometers coupled to ion-mobility devices have facilitated the accurate measurement of peptide collisional cross sections (CCS) adding an extra dimension to proteomics investigations. Recently, a method that do not require a dedicated ion mobility cell was described for determining CCS values of protein ions based on their decay rates in the time-domain transient signal in an Orbitrap mass analyzer. Here we present an extension of this strategy to peptide CCS inference in complex proteomics samples by introducing a minor hardware modification to an Orbitrap Exploris 480 mass spectrometer. We benchmarked the performance of this approach by analyzing HeLa lysates comparing to previously published CCS values from ion-mobility datasets showing comparable results.
MP 176 – Inference of Collisional Cross-Sections of peptides in an Orbitrap Mass Analyzer
by Eva Bryderup