dia-PASEF (Meier et.al.,2019) takes advantage of the additional dimension of separation provided by trapped ion mobility (TIMS) for the analysis of complex proteomics samples by data independent analysis (DIA). The CCS (Collisional Cross Section) value determined for each precursor can also be used for alignment of precursor and fragment information. ­­ Here, we evaluate benefits of dia-PASEF comparing very short and long gradients. Moreover, we compared results from two independent software platforms (Spectronaut 15 and DIA-NN 1.8) which can process native dia-PASEF data using spectral libraries or a library-free approach.