Reconnect

Join Evosep at HUPO 2021

Meet Evosep at this year’s HUPO 2021

This year HUPO ReCONNECT will be a fully virtual conference from November 15-19. Using the virtual platform Gather.Town it will be possible for delegates to talk to each other using video chat and walk around a virtual hallway to mingle.

Though virtual, this year’s conference still presents a strong program with Plenary & Keynote Sessions as well as Hot Topic Discussion Groups and Industry Seminars. We also have some exciting activities planned and as a virtual event there really is no excuse not to be there. W

All week you can meet us and we would love to talk to you and learn more about you and your current research. We are also more than happy to introduce you to our technology.

Scientific presence

We are excited to advocate several interesting customer talks during this year’s HUPO conference. Learn more about them below and find the full list of all abstracts featuring Evosep One.

Talks

Hot topic discussion

Standardization is key (for sample separation for MS based proteomics analysis)

Reviewing the methods sections in published papers, it looks like everybody has their own standard or optimized solution. But the variety makes it difficult to reproduce the experiments, makes it harder to compare results, and slows down knowledge transfer. The question then is whether standardization will hurt innovation or enable a deeper focus on the biological mechanisms under study.

Discussants:

Jennifer Van Eyk, Cedars-Sinai Medical Center, Los Angeles, USA

Phil Robinson, CMRI & ProCan, Sydney, Australia

Martin Overgaard, SDU & Odense University Hospital, Odense, Denmark

Nicolai Jacob Wewer Albrechtsen Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark

When: Nov 17, 2021, 16:00-17:00 UTC

Determining calibration curves for robust, targeted, and high throughput proteomics with an Evosep One and Agilent 6495C LC/TQ

Talk by Wendi Hale, Ph.D., LC/MS Applications Scientist at Agilent

When transitioning from discovery proteomics to targeted protein quantification, the multiple reaction monitoring (MRM)-based LC/MS method plays an essential role. Sample complexity and the low concentration of certain proteins are the major challenges encountered when performing protein analysis using targeted methods.

Recent advancements in low-flow HPLC and acquisition rates in QQQ instrumentation is enabling MRM-based analytical methods using stable isotope-labeled standard (SIS) peptides for targeted quantitative proteomics to improve sensitivity, linear range, precision, accuracy, robustness, and most importantly throughput in the data acquisition as well as simplified data processing. Using a commercially available complex proteomics sample (PeptiQuant Plus 125 from MRM Proteomics) spiked in a plasma matrix, calibration curves were established highlighting the significant quantitative advantages of MRM-based targeted quantification. In addition, a select number of peptides from the complex proteomics sample were quantified with very high productivity methods combining the use of Evosep One and Agilent 6495C LC/TQ. Quantification with 3- and 5-min low-flow gradients (0.15 mm ID column) and 4-5 min re-equilibrations are demonstrated with short dwell times offering excellent quantitative performance with high throughput. Sensitivity, linearity, and precision is demonstrated with these fast low-flow methods demonstrating inject-to-inject cycle times under 10 minutes. This study demonstrated the suitability of this new high-performance LC/MS system configuration for high throughput protein quantification, even with very limited sample amount.

When: On-demand seminars

Abstracts

poster

A complete and automated sample preparation strategy for high throughput and standardized proteomics

Dorte B. Bekker-Jensen1, Florian S. Harking², Angela McArdle¹, Lasse Falkenby¹, Jakob Bunkenborg¹, Ole B. Hørning¹, Jesper V. Olsen², Nicolai Bache¹

¹ Evosep Biosystems, Denmark, ² Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark

Download

C.P. 08 – corporate poster

A Standardized Separation Tool for Clinical Omics

Dorte bekker-Jensen, Evosep

P09.28 – Abstract

Dynamic Polygon for MHC Class I and II Immunopeptides

Dr. Francesco Pingitore, Michael Krawitzky, Josh Elias, Chris Adams, Bruker

P10.18 – Abstract

Biomarker Monitoring in Body Fluid by High Sensitivity and High Throughput FAIMS-SurequantTM Is Targeted Quantitation

Mr. Konstantinos Kalogeropoulos1, Dr. Simonas Savickas1, Miss Cathrine Agnete Larsen1, Mr. Aleksander Moldt Haack1, Dr. Erwin Shoof1, Dr. med. Hans Smola2, Dr. Louise Bundgaard1, Dr. Ulrich auf dem Keller1 1Technical University of Denmark, 2Paul Hartmann AG

P12.B08 – Abstract

Evaluation of Dia-PASEF Using Library and Library Free Approaches for Different Gradients

Dr. Diego Assis, Dr. Elizabeth Gordon , Dr. Matthew Willetts, Bruker Scientific LLC

P12.B15 – Abstract

Impact of Improved MS/MS Duty Cycle On Protein Identification Efficiency using Data Independent Acquisition On a New QTOF Platform

Mr. Ihor Batruch, Yves Le Blanc, Stephen Tate, Jason Causon, Nick Morrice, Naomi Diaz, Tatjan Talamantes, Anjali Chelur, Nic Bloomfield, Pavel Ryumin, Jose Castro-Perez, SCIEX,

P12.C10 – Abstract

Label-Free Quantification of Oxidized Peptides in eHAP Cell Lines via a High-Throughput Dia-PASEF Workflow

Dr. Romano Hebeler1, Dr. Renata Blatnik1, Dr. Philipp Strohmidel1, Hamish Pegg2, Dr. Joanna Kirkpatrik2
1Bruker Daltonics GmbH & Co. KG, 2The Francis Crick Institute

P12.C51 – Abstract

DeGlyPHER: An Ultrasensitive Method for Analysis of Viral Spike NGlycoforms

Dr. Sabyasachi Baboo 1, Dr. Jolene Diedrich1, Dr. Salvador Martinez-Bartolome 1, Dr. Xiaoning Wang 1, Dr.Torben Schiffner 1,2,3, Dr. Bettina Groschel 1,2, Prof. Dr. William Schief1,2,3, Prof. Dr. James Paulson 1, Prof. Dr. John Yates 1

1The Scripps Research Institute, 2 IAVI Neutralizing Antibody Center, The Scripps Research Institute, 3The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard

Science and Technology award

HUPO is special to us as this was where we first launched the Evosep One back in 2017. Since then, a lot has happened. We have move to a more spacious office and many new faces have joined the team.

This year we are honoured that our contribution to the community has been rewarded with the Science and Technology award in the field of proteomics. This work was spearheaded by Founder of Evosep Ole Vorm and Head of Applications Nicolai Bache.

LET’S GET IN TOUCH

We are always open to answering questions and form new collaborations, so don’t hesitate to reach out. Fill out our contact form and we’ll do our very best to assist you.