Here you can find the latest publications from Evosep, our users and collaboration partners.
Advanced Host Cell Protein (HCP) Analysis with TIMS QTOF MS Powers Biopharmaceutical Development
This recent study from Bruker Daltonics looks into the ever increasing requirement for analytical methods with rigorous impurity testing, to monitor contaminants during manufacture and prior to product release.
Peptidomic analysis of urine from youths with early type 1 diabetes reveals novel bioactivity of uromodulin peptides in vitro
Using unbiased and targeted mass spectrometry-based techniques this paper aimed to predict peptide bioactivity and to identify the potential proteases responsible for generating the peptides.
Julie A. D. Van1, Sergi Clotet-Freixas, Joyce Zhou, Ihor Batruch, Chunxiang Sun, Michael Glogauer, Luca Rampoldi, Yesmino Elia, Farid H. Mahmud, Etienne Sochett, Eleftherios P. Diamandis, James W. Scholey, Ana Konvalinka
Accurate Detection of the Four Most Prevalent Carbapenemases in E. coli and K. pneumoniae by High-Resolution Mass Spectrometry
A team at Erasmus MC have developed a new method to accurately and rapidly detect specific resistance mechanisms simultaneously by combining high-resolution mass spectrometry with liquid chromatography.
Dimard E. Foudraine, Lennard J. M. Dekker, Nikolaos Strepis, Michiel L. Bexkens, Corné H. W. Klaassen, Theo M. Luider, Wil H. F. Goessens
Rapid Host Cell Protein (HCP) Detection with Advanced
Trapped Ion Mobility Spectrometry (TIMS) Technology
The robust and reproducible 21 min gradient on the Evosep One instrument provides rapid analysis of downstream processes, with fast runs and high throughput, enabling batch-to-batch fingerprinting. Quantitation is often necessary and the faster analysis with Bruker’s timsTOF Pro is advantageous for more routine applications with coverage of 50+ HCPs at 1% false discovery rate (FDR).
Authors: Guillaume Tremintin, Stuart Pengelley, Michael Greig
Scalable and Automated Plasma Workflow Based on the Q Exactive HF -X MS Platform
Read about our recent developments with the Thermo Fisher Scientific Precision Medicine Science Center and see the results of our joint work to develop standardized workflows for large cohort plasma profiling.
Authors: Jing Wang, Sarah Trusiak, Ryan D. Bomgarden, Sergei Snovida, Emily I. Chen, Thermo Fisher Scientific Precision Medicine Science Center
Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites
The need for scalable methods for functional annotation of phosphotyrosine sites has led the Olsen group, Novo Nordisk Foundation Center for Protein Research in Copenhagen, to develop a new mass spectrometry-based workflow for high-throughput analyses.
Authors: Alicia Lundby, Giulia Franciosa, Kristina B. Emdal, Jan C. Refsgaard, Sebastian P. Gnosa, Dorte B. Bekker-Jensen, Anna Secher, Svetlana R. Maurya, Indranil Paul, Blanca L. Mendez, Christian D. Kelstrup, Chiara Francavilla, Marie Kveiborg, Guillermo Montoya, Lars J. Jensen, Jesper V. Olsen
Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform
Together, Thermo Fisher Scientific and Evosep are on a joint mission to develop standardized and scalable plasma protein quantitative profiling workflows. Our recent developments have been featured in this application note by the Precision Medicine Science Centre led by Dr. Emily Chen.
Authors: Jing Wang, Sarah Trusiak, Ryan D. Bomgarden, Sergei Snovida, Michelle Dubuke, Emily I. Chen
Study: Increased De Novo Protein Sequencing Coverage with Optimal Protease Cocktail
This study from Rapid Novor Inc presents how they de novo sequence 166 mAb using Evosep One coupled to Thermo Fisher Scientific Orbitrap Fusion Series instrument.
Authors: Le Bihan, T., Taylor, P., McDonald, Z., Liu, Q., Shen, J., Gorospe, K., Xu, X., Hosfield, C., Ma, B.
A NOVEL LC SYSTEM EMBEDS ANALYTES IN PRE-FORMED GRADIENTS FOR RAPID, ULTRA-ROBUST PROTEOMICS
This paper presents a novel concept that opens entirely new possibilities in proteomics in general and in clinical proteomics. With a simplified design it enables a robust operation over thousands of sample injections and analyzes samples in an extremely robust and high throughput manner, without sacrificing in depth proteomics coverage.
Authors: Nicolai Bache, Philipp E. Geyer, Dorte B. Bekker-Jensen, Ole Hoerning, Lasse Falkenby, Peter V. Treit, Sophia Doll, Igor Paron, Johannes B. Muller, Florian Meier, Jesper V. Olsen, Ole Vorm, and Matthias Mann
Plasma proteomics goes high throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min
The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma.
Authors: Thomas Kosinski, Raphael Heilig, Dalila Bensaddek, Nicolai Bache, Ole Bjeld Hørning, Roman Fischer, Heiner Koch
Evosep One Enables Robust Quantitative Deep Proteome Coverage using Tandem Mass Tags while Significantly Reducing Instrument Time
This paper by Jonathan Krieger, PhD and collagues shows how Evosep One Enables Robust Quantitative Deep Proteome Coverage using Tandem Mass Tags while Significantly Reducing Instrument Time.
Authors: Jonathan R. Krieger, Leanne E. Wybenga Groot, Jiefei Tong, Nicolai Bache, Ming S. Tsao, Michael F. Moran
Online parallel accumulation − serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer
In bottom−up proteomics, peptides are separated by liquid chromatography with elution peak widths in the range of seconds, while mass spectra are acquired in about 100 microseconds with time−of−fight (TOF) instruments.
Authors: Florian Meier, Andreas-David Brunner, Scarlet Koch, Heiner Koch, Markus Lubeck, Michael Krause, Niels Goedecke, Jens Decker, Thomas Kosinski, Melvin A Park, Nicolai Bache, Ole Hoerning, Jürgen Cox, Oliver Räther and Matthias Mann
High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition
To support proteomics studies the Evosep One combined with Thermo Scientific™ Q Exactive™ are used to develop High-throughput LC-PRM set-ups based on fast LC separations and advanced PRM acquisition schemes. A novel IS-PRM method variant was also developed to further increase the analytical throughput and open the way to fast broad-coverage/multi-pathway monitoring studies.
Authors: Sebastien Gallien, Aaron Gajadhar, Bhavin Patel, Tabiwang Arrey, Dave Sarracino, Yue Xuan, Emily Chen
MAXIMIZED THROUGHPUT AND ANALYTICAL DEPTH FOR SHOTGUN PROTEOMICS USING PASEF ON A TIMS EQUIPPED QTOF
While good analytical depth is currently achievable in (MS)-based proteomics, the throughput required in screening or clinical applications is not achievable with traditional nanoESI LC-MS/MS systems. Combining the Evosep One with low overhead times and the timsTOF Pro with the fast PASEF acquisition method (Meier et al., JPR 2015). We show highly reproducible identification and quantification with low sample amounts over ~100 runs, demonstrating the potential for high throughput applications.
Authors: Thomas Kosinski; Scarlet Koch; Markus Lubeck; Nicolai Bache; Ole Bjeld Horning; Lasse Falkenby; Heiner Koch
HIGH THROUGHPUT HCP SCREENING IS HERE: > 50 HCPS IDENTIFIED IN 21 MINUTES
Here, the high speed of PASEF, (parallel accumulation and serial fragmentation) as implemented on the timsTOF Pro, coupled with the Evosep One LC system were used to detect HCPs using a 21 minute gradient.
Authors: Stuart Pengelley, Heiner Koch, Thomas Kosinski, Eckhard Belau; Bruker Daltonik, Bremen
As the protein concentration in plasma is so high (50 μg/μl), a simple fingerprick delivering just 1μl of plasma is enough to analyze the human plasma proteome.
Authors: Philipp E. Geyer, Lesca M. Holdt, Daniel Teupser and Matthias Mann
DEVELOPMENT OF A ROBUST AND REPRODUCIBLE METHOD FOR DETECTION OF CITRULLINATION IN COMPLEX SAMPLES
An initial separation by traditional LC using HFBA as ion-pairing reagent followed by short LC-MSMS separation on an EVOSEP system is compared to a traditional LC-MS/MS setup, with the aim of developing a system, where specific ion pairing reagents are used to separate arginine, containing peptides from equivalent citrullinated ones.
Authors: Daniel Nyberg Larsen, Christian Engelbrecht Mikkelsen, Jan Potempa, Jakub Z. Kaczmarek, Peter Højrup
DEVELOPMENT OF A NOVEL LC CONCEPT FOR CLINICAL PROTEOMICS
Mass spectrometry based proteomics and metabolomics are fast growing and powerful technologies, with the potential to revolutionize health care and precision medicine. Here we describe a conceptually novel low-flow chromatography system, that delivers the robustness and throughput required for clinical applications while maintaining the sensitivity of current nano-flow LC instrumentation.
Authors: Nicolai Bache, Philipp E. Geyer, Ole Hoerning, Lasse Falkenby, Peter Treit, Igor Paron, Florian Meier, Ole Vorm and Matthias Mann
OPTIMIZING DATA PROCESSING PARAMETERS FOR SAMPLES ACQUIRED ON INSTRUMENTS USING ADVANCED PEAK DETERMINATION
Thermo and Evosep’s data from the joint use of the Evosep One system and Thermo’s QExactive mass spectrometers. The samples were measured using an Evosep One coupled to an Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer, and the Evotip technology improves overall system throughput and robustness by reducing column clogging and carryover.
Authors: Bernard Delanghe; Tabiwang N. Array; Aaron Gajahar; David Horn; Pedro Navarro; Eugen N. Damoc; Andreas Huhmer
TIMSTOF PRO WITH PASEF AND EVOSEP ONE: MAXIMIZING THROUGHPUT, ROBUSTNESS AND ANALYTICAL DEPTH FOR SHOTGUN PROTEOMICS
The recent release of Evosep one, has changed the game, ceasing the need for concatenation and cutting MS analysis time in half. The results presented here demonstrate combining the timsTOF Pro and the Evosep One, for the first time enables high-throughput measurements while maintaining analytical depth.
Authors: Thomas Kosinski; Scarlet Koch; Markus Lubeck; Pierre-Olivier Schmit; Nicolai Bache; Ole Bjeld Horning; Lasse Falkenby; Heiner Koch