Whisper is the latest Evosep One innovation that allow plug-and-play, split-free 100 nl/min performance. The new methods make use of Evosep’s unique low pressure gradient formation and very accurate flow control on the same standard system without technical modifications.
The gradient is built and stored in the loop in the low pressure part of the system and then pushed to the analytical column using the isocratic high pressure pump, allowing for accurate gradients at very low flow. The Whisper methods are designed for ultra high sensitivity applications, where sample amounts are very limited. The methods are freely available through a software upgrade for standard Evosep One hardware, while optimized columns and emitters are required.
Learn more about the advantages of the pioneering Whisper™ flow technology in this webinar.