High-Resolution Mass Spectrometry

By Team Evosep

High-Resolution Mass Spectrometry

Facilitating early adequate therapy for patients infected by multidrug resistant bacteria calls for a novel precise and rapid diagnostic method as today’s resistance determinations are often slow and expensive.

A newly released paper by a team at the Erasmus Medical Center in Rotterdam display a new MS based method as an alternative to PCR and immunoassays. By combining highresolution mass spectrometry with liquid chromatography (LC) they have developed a method to accurately and rapidly detect specific resistance mechanisms simultaneously.  

Figure 1. Overview of the LC-MS/MS pre-treatment used. 

Targeted bottom-up mass spectrometry

The method was developed using targeted bottom-up mass spectrometry in order to accurately detect the four most prevalent carbapenemases in a single analysis. Digests were loaded onto Evotips, separated using the Evosep One and the 100 samples per day method. Detection was performed using an Orbitrap mass spectrometer.

The Evosep One allowed them to reduce turnaround time making method development faster and the methodology more fitting for routine diagnostics and decreasing hands-on time per sample. Also, no carryover was detected making blank runs redundant.

This new LC-MS/MS method could potentially be used to rapidly detect many other resistance mechanisms in a single assay as well.