Evosep Webinar series
Spring 2021
FEB 25th 2021 I 4:00 pm CET I Covid-19 research with Evosep One
MAR 25TH 2021 I 4:00 PM CET I HOST CELL PROTEIN Analysis With Evosep One
APR 29TH 2021 I 4:00 PM CET I Protein-Protein interaction Analysis With Evosep One
Join us once a month when we zoom in on a new topic and invite Evosep One users and collaborators to share their work. We hope these events will become a popular platform where Evosep One users can share their research with the rest of the world.
We would love to stay relevant to you and learn about your research goals and and maybe you have a topic you would like us to cover in a future webinar? Get in touch with us at info@evosep.com
Rapid, robust and reproducible Host Cell Protein analysis with Evosep One
MAR 25th 2021 I 4:00 pm CET I 10:00 am EST
In this webinar we will explore the possibilities of using the Evosep One technology to perform rapid, robust and reproducible HCP analysis.
Program
Full speaker line up will be announced soon.
Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development
Talk by Jiao Ma, Ph.D. and Scientist II, AstraZeneca
There is a growing industry and regulatory need to detect host cell proteins (HCPs) in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safely and efficacy, even at low levels. We developed an HCP profiling workflow using an Evosep ONE LC system coupled to an Orbitrap Fusion Lumos mass spectrometer, which lead to HCP detection and quantification in a significantly shorter turnaround time.
The workflow can be readily implemented and adapted for different purposes to guide biopharmaceutical process development and enable better risk assessment of HCPs in drug substances.
Title to be announced
Talk by Anders Giessing, Senior Scientist hos Novozymes
More information will follow.
Robust and comprehensive Protein-Protein Interaction analysis with the Evosep One
Apr 29th 2021 I 4:00 pm CET / 10:00 am EST
Program
Introduction
Introduction and Talk by Nicolai Bache, Head of applications, Evosep
An atlas of protein-protein interactions across mouse tissues
Talk by Leonard Foster, Assistant Prof. at University of British Columbia
Cellular processes arise from the dynamic organization of proteins in networks of physical interactions. Mapping the complete network of biologically relevant protein-protein interactions, the interactome, has therefore been a central objective of high-throughput biology. However, the dynamics of protein interactions across physiological contexts remain poorly understood.
Here, we develop a quantitative proteomic approach combining protein correlation profiling with stable isotope labelling of mammals (PCP-SILAM) to map the interactomes of seven mouse tissues. The resulting maps provide the first proteome-scale survey of interactome dynamics across mammalian tissues, revealing over 125,000 unique interactions at a quality comparable to the highest-quality human screens. We identify systematic suppression of cross-talk between the evolutionarily ancient housekeeping interactome and younger, tissue-specific modules. Rewiring of protein interactions across tissues is widespread, and rewired proteins are tightly regulated by multiple cellular mechanisms and implicated in disease.
Our study opens up new avenues to uncover regulatory mechanisms that shape in vivo interactome responses to physiological and pathophysiological stimuli in mammalian systems.
In-Cell Protein Footprinting Coupled with Mass Spectrometry for Structural Biology Across the Proteome
Talk by Lisa Jones, Associate Professor at University of Maryland, Baltimore (UMB)
In recent years, protein footprinting coupled with mass spectrometry has been used to identify protein-protein interaction sites and regions of conformational change through modification of solvent accessible sites in proteins.
The footprinting method, fast photochemical oxidation of proteins (FPOP), utilizes hydroxyl radicals for protein modification. To date, FPOP has been used in vitro on relatively pure protein systems. We have further extended the FPOP method for in vivo analysis of proteins both in cells and in C. elegans, an animal model for human disease. We have optimized experimental conditions so that the method can modify thousands of proteins for structural biology across the proteome.
Past events
Covid-19 research with Evosep One
available on demand
The global emergence of novel coronavirus disease 2019 (COVID-19) has underlined the requirement for rapid assays with high sensitivity and specificity for detection of infection and further to understand the molecular complexity of the disease.
In this webinar you will meet Evosep One users who have used our technology in Covid-19 assay development and to gain a comprehensive understanding of the disease.
Program
Evosep Columns
Introduction and Talk by Nicolai Bache, Head of applications, Evosep
Development of Robust High throughput DIA Workflow for Detection of Protein Signatures for COVID-19 Positivity and Progression
Talk by Angela Mc Ardle, Project Scientist, Cedars-Sinai
Analysing SARS-COV-2 samples by fast and sensitive mass spectrometry
Talk by Matthias Trost, Professor of Proteomics, Newcastle university
A beginner’s guide to Evosep
Available on demand
If you are new to our technology or just getting started this event is for you. We will introduce the basics of the Evosep One technology and include hot-from-the-lab data from our users. Nicolai Bache, Head of Applications at Evosep, will host the event, bringing you up to speed with the latest additions to the Evosep One technology.
Program / Speakers
The fundamentals of evosep one
Introduction and Talk by Nicolai Bache, Head of applications, Evosep
CETSA MS Profiling for a Comparative Assessment of FDA-Approved Antivirals Repurposed for COVID-19 Therapy Identifies TRIP13 as a Remdesivir Off-Target
Talk by Alexey Chernobrovkin, Senior Research Scientist, Pelago Bioscience
Assessing Parkinson’s Disease-linked LRRK2 kinase activity by multiplexed targeted mass spectrometry
Talk by Raja Nirujogi, Post Doc, University of Dundee
WHISPER IS COMINg
Available on demand
Whisper is the latest Evosep One innovation that allow plug-and-play, split-free 100 nl/min performance. The new methods make use of Evosep’s unique low pressure gradient formation and very accurate flow control on the same standard system without technical modifications.
The gradient is built and stored in the loop in the low pressure part of the system and then pushed to the analytical column using the isocratic high pressure pump, allowing for accurate gradients at very low flow. The Whisper methods are designed for ultra high sensitivity applications, where sample amounts are very limited. The methods are freely available through a software upgrade for standard Evosep One hardware, while optimized columns and emitters are required.
Learn more about the advantages of the pioneering Whisper™ flow technology in this webinar.
Program / Speakers
Robust, very low nanoflow LC-MS methods for ultra sensitive single-cell and phosphoproteomics analysis
Introduction and Talk by Nicolai Bache, Head of applications, Evosep
