FFPE tissue analysis on the Evosep One
available on demand
Formalin fixation and paraffin-embedded (FFPE) is the most common way to preserve clinical specimens and archived tissue banks represent a huge potential for protein biomarker studies. Proteins are stable in FFPE, but still challenging to extract for proteomics workflows.
In this webinar, we focus on how robust workflows combined with short gradients on the Evosep One is a perfect match for the analysis of large cohorts of patient samples.
Evaluation of an Evosep One -FAIMS – Exploris trio for the proteomic analysis of FFPE tissue
Talk by Bernhard Kuster, Prof. Dr., Chair of Proteomics and Bioanalytics, Technical University of Munich
Proteomic biomarker discovery using archived (FFPE) tissue requires robust workflows to support the analysis of large cohorts of patient samples and we evaluated the merits of an EvosepOne-FAIMS-Exploris trio for this purpose. The data shows that this setup performs on par with StageTip-based off-line fractionation but requires 50 % less sample and substantially reducing sample handling. Using FFPE material from lymph node, lung and prostate tissue as examples, we show that nLC-FAIMS-MS/MS can identify 5-6,000 proteins from the respective tissue within a total of 3 hours of analysis time.
Enabling fast proteomic profiling of COVID-19
Talk by Lisa Schweizer, PhD Student, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry
Streamlined data acquisition is a pre-requisite to target clinical research questions by MS-based proteomics. Most currently, the ongoing pandemic of COVID-19 has exemplified this requirement and the urgent need for deep biological understanding of the disease. Employing the EvosepOne LC system, we implemented rapid and universal strategies for the analysis of diverse patient samples to explore the proteome of COVID-19. Our data display widespread inflammatory processes, a hallmark of severe COVID-19, as well as organ-specific effects.