Evosep webinar

ASMS 2022 Highlights: Robust and high-throughput analysis with ultra-high sensitivity

Available On Demand

We are back from an insightful ASMS 2022 with interesting new science and a fresh view on the updates in the community.

At this webinar, we shared our impressions from the conference and new data, which we presented during the conference.

RAPID AND ROBUST PROTEOMES WITH STANDARDIZED METHODS ON THE EVOSEP ONE POWERED BY ZENO-SWATH

We evaluated the new ZenoTOF 7600 mass spectrometer with the novel ZenoSWATH acquisition strategy across the Evosep One standard methods. For high-throughput applications with 200 samples analyzed per day, we were able to reproducible identify and quantify more than 4,000 proteins from 200 ng HeLa digest.

Collectively our results show that ZenoSWATH is a significant driver of deep proteomes, making the ZenoTOF 7600 an ideal match with high-throughput and standardized methods on the Evosep One.

ULTRA-HIGH SENSITIVITY FOR TARGETED PROTEOMICs USING EVOSEP-MRM AT the SINGLE CELL LEVEL

The robustness and throughput of the Whisper methods on the Evosep One combined with the sensitivity of the Agilent 6495C triple quadrupole support targeted analysis of proteomes at the single cell level. We demonstrated this by targeting selected peptides in a complex background of sub nanogram material combined with dilution curves spanning several orders of magnitude using the standard 60 SPD method and the Whisper 40 SPD method.

We found that the Whisper method provided a fourfold boost in sensitivity and applied this to targeted analysis of sorted single cells.

ROBUST AND HIGH-THROUGHPUT SINGLE CELL ANALYSIS WITH THE EVOSEP ONE

Recent advancements and optimization of Whisper Flow technology combined with a new generation Evotip provide efficient capture and recovery of peptides from especially low sample amounts down to the single cell level. Additional optimizations include a new 15 cm column from IonOpticks (75 µm ID with 1.7 µm beads, Generation 3 Aurora Elite), which is perfectly suited for disposable trap columns with a slightly more hydrophobic material. This includes improved chromatographic performance, where slimmer peaks result in more than 50% higher peptide coverage more than 2,000 proteins identified from sorted single cells. Additionally, we will discuss how to accelerate the sample throughput to enable analysis of larger cohorts of single cells.

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