Evosep webinar

Fractionation

Thursday, October 27, 2022, 16:00 CEST / 10:00 AM EDT

Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the peak capacity of the separation. This leads to increased dynamic range of the identified peptides compared to conventional single-shot analysis.

The additional number of injections per fractionated sample is a good match with the short overhead time between injections on the Evosep One.

SPEAKERS 

Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing

Talk by Claire Koenig, CPR

Combination of isobaric labeling (TMTs) coupled with offline high-pH fractionation can maximize analysis depth in quantitative phosphoproteomics analysis. To investigate to what extent sample amount affect the sensitivity and the dynamic range of the analysis, we benchmark strategies for quantitative phosphoproteomics with limited peptide input amounts. Based on this research, we present a decision tree to guide phosphoproteomics users to find the best workflow, to reach optimal depth of coverage, based on experimental design and peptide availability.

More to be announced