Available on demand
Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. This leads to increased dynamic range of the identified peptides compared to conventional single-shot analysis.
The additional number of injections per fractionated sample is a good match with the short overhead time between injections on the Evosep One.
Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
Talk by Claire Koenig, CPR
Combination of isobaric labeling (TMTs) coupled with offline high-pH fractionation can maximize analysis depth in quantitative phosphoproteomics analysis. To investigate to what extent sample amount affect the sensitivity and the dynamic range of the analysis, we benchmark strategies for quantitative phosphoproteomics with limited peptide input amounts. Based on this research, we present a decision tree to guide phosphoproteomics users to find the best workflow, to reach optimal depth of coverage, based on experimental design and peptide availability.
Fractionation to explore cellular protein complex assembly states at high throughput
Talk by Moritz Heusel, Evosep
Fractionation in proteomics typically aims at reducing sample complexity to access lower-abundant polypeptide species and improve proteome coverage. However, fractionation has also proven very useful to understand organizational attributes of cellular systems and the proteome such as i) subcellular organization and ii) organization into complexes as key functional modules. In this talk we will touch on the merits of fractionation to assess proteome organization, benefits of fractionated/extended spectral libraries and merits of rapid Evosep LC to process these large sample sets.