In this exciting webinar, you’ll get an overview of our recent advancements to enhance the efficiency and accessibility of proteomics combined with scalable proteomics workflows.

Dive into our fully automated workflows, the new Whisper Zoom methods, and the grand opening of Evosep Biolabs.

Host: Nicolai Bache, VP Proteomics Research at Evosep

Increasing the throughput of label-free single-cell proteomics analysis using Whisper Zoom Gradient on Evosep One

Talk by Pierre Camille Sabatier, Guest Researcher, Olsen Group, Novo Nordisk Foundation Center for Protein Research

Label-free single cell proteomics currently suffers from a lack of throughput which could be achieved by faster chromatography methods. However, reducing gradient length while maintaining low flow rates to preserve proteome coverage and improve sensitivity is challenging. We investigated the applicability of the newly implemented 80 and 120 SPD whisper gradients for label-free single cell analysis of HeLa and human pluripotent stem cells and compared the performance with the 40 SPD whisper gradient. Overall, we double the throughput while identifying slightly less protein with the 80 SPD gradient and still detect low-abundant stem cell transcription factors relevant for biological applications.

Standardized end-to-end Proteomics Solution with Evosep One and Liquid Handling Robots

Talk by Dorte Bekker-Jensen, Head of Application Development at Evosep

Proteomics is undergoing a paradigm shift and rapidly evolving towards more efficient and user-friendly technologies. The Evosep One, combined with improvements in high-end mass spectrometry, has played a vital role in transforming this field, making it possible to analyze hundreds of samples daily. Consequently, the bottleneck in high-throughput proteomics has shifted towards sample preparation with a demand for a robust pipeline with automated end-to-end workflows.

We have used the opportunity to miniaturize the workflow and only digest the sample amount required for analysis. This is achieved by loading the peptide digest directly on the Evotip for complete integration with sample storage and downstream LC-MS analysis.​ This is a cost-efficient approach and sustainable by design, through significant reduction in enzyme usage and plasticware consumption, enabled by the Evotip.

The talk will highlight a selection of workflows for standard proteomics, which can be exploited for a number of liquid handlers including standard protein lysate digestion and plasma workflows.

Key Highlights:

Fully Automated Workflows

Learn how our automated workflows can process over 500 plasma samples per day, maximizing efficiency and throughput while minimizing manual labor and potential errors. These workflows are a game-changer for drug discovery and translational research.

New Whisper Zoom Methods

Discover the enhanced scalability and sensitivity of our Whisper Zoom methods. This innovative technology supports the development of proteomics-based diagnostics by providing more detailed and accurate results.

Evosep Biolabs

We’re proud to announce the opening of Evosep Biolabs, a center of excellence for high-throughput proteomics. Specializing in the proteomic analysis of plasma samples, Evosep Biolabs leverages automated sample preparation and standardized separation methods to deliver exceptional analytical services.

Don’t miss this opportunity to stay ahead of the curve in proteomics research.