in ChinaGet insight into the latest Evosep technology and hear from experts in the field how this is used to address key biological questions
Evosep in China
We are happy to meet and engage with the Chinese proteomic communities.
On this website you can find information on the Evosep One technology, as well as download selected application notes and our sample loading protocol in Mandarin below.
INTEGRATED PURIFICATION AND SEPARATION DELIVERED BY THE EVOSEP ONE
The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
“Robust and fast workflows are indispensable for successfully implementing large-cohort clinical plasma proteomics studies”,
Professor Matthias Mann, Max-Planck Institute of Biochemistry,
Proteomics and Signal Transduction
Evotip Pure: a New and improved generation of Evotip
Evotip Pure™ is a new generation Evotip developed for high performance. To ensure quality, we have internalized and refined all critical manufacturing and carefully chosen the chemistry.
The design of Evotip Pure is based on five years of customer feedback added to a thorough review of every single aspect of the Evotip.
Better chromatography with Evotip Pure
The new Evotip Pure will not only provide much better chromatographic performance but also ensure improved results and consistency over time.
The improved peak definition and peak width will significantly increase peak capacity and ultimately lead to better performance.
The new Evotips will give you more efficient sample binding, significantly improved sensitivity at low loads and visibly sharper and more symmetric peaks at all loads. Retention times are identical +/- 1 second using the 60 SPD method.
The improved chromatographic performance is shown above where sharper and more symmetric peaks leads to significantly improved peak capacity.
HOW FAST WILL YOU GO?
The speed of state-of-the-art mass spectrometers combined with the low overhead time and robustness on the Evosep One is a perfect match for high utilization. It is therefore needed to carefully review the balance of proteome depth versus high throughput before starting a series of experiments.
ENHANCED PROTEOME COVERAGE WITH THE PERFORMANCE COLUMN
The performance column is available for the 60 and 100 samples per day methods and is packed with 1.5 μM C18 beads. This reduces the peak widths with 2 seconds at FWHM and leads to an increase in peak capacity of 60%. Consequently, the proteome coverage is enhanced, and the column provides a robust and competitive commercial option – on the same level as home-pulled and packed options, but with better reproducibility and life-time. The boost in sensitivity is particularly noticeable for lower loads, where peptide and protein coverage is improved with 100%.
A BEginner’s Guide to Evosep
If you are new to our technology or just getting started this event is for you. We will introduce the basics of the Evosep One technology and include hot-from-the-lab data from our users. Nicolai Bache, Head of Applications at Evosep, will host the event, bringing you up to speed with the latest additions to the Evosep One technology.
Application notes in mandarin
Evotip Pure 通过出色的可重复性, 存储性能和回收率简化工作流程
End to End, 全自动酶解步骤及Opentrons OT-2上的Evotip Pure工作流程
用于简化Evotip Pure的样本上样操作的全自动Opentrons OT-2加载流程
使用Evotips可简化工作流程，省 略多个样品处理步骤，并减少系统 等待时间。
通过以非常精确和可重复的部分洗 脱方式，可以将交叉残留污染降到 最低，并防止杂质和分子物质进入 Evosep One。
Meet the team
VP Proteomics Research
Chief Executive Officer
VP EMEA and APAC