The recently introduced Whisper™ Flow Technology uses preformed gradients and disposable Evotip trap columns with the sample preloaded and immobilized to robustly and reproducibly handle very small sample amounts, down to actual single cells, at sub-100 nl/min...
This application note by Bruker describes the timsTOF SCP instrument and demonstrates robust proteome coverage down to 250 pg of peptide using the Whisper 40SPD method.
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