Clinical Proteomics must be Sensitive, Fast, and Robust,
which Evosep One achieves with perfection
INTEGRATED PURIFICATION AND SEPARATION DELIVERED BY THE EVOSEP ONE
The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
HOW FAST WILL YOU GO?
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.
The speed of state-of-the-art mass spectrometers combined with the low overhead time and robustness on the Evosep One is a perfect match for high utilization. It is therefore needed to carefully review the balance of proteome depth versus high throughput before starting a series of experiments.
STANDARD PROTEOMICS METHODS OPTIMIZED FOR FAST, HIGH-PERFORMANCE SEPARATION
Our five standard methods cover a broad range of applications. Explore more and find your favorite.
30 SPD: The longest method with the best overall proteome coverage. Download app note
60 SPD: The most popular method with an excellent balance of throughput and depth. Download app note
100 SPD: A great “in-between” method. Download app note
200 SPD: Excellent sequencing speed with most peptides per minute among our standard methods. Download app note
300 SPD: The highest peak capacity per minute with our fastest method for ultra-high throughput analysis. Download app note
IT’S ALL IN THE EVOTIP
The Evotip, a disposable trap column, is the core of our technology. The Evotip serves as an integrated purification step, where contaminants are left on the tip, and analytically relevant peptides are eluted.
This minimizes cross-contamination and extends column lifetime. The Evotip has proven to be extraordinary important for ultra-sensitive analysis, as very small sample amounts can be loaded in higher volumes.
Additionally, loaded Evotips can be stored cold prior to analysis.
Built for standardization
The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.
Illustrated below using the 100 samples per day method and the standard column (EV-1064).
“Single cell proteomics is becoming a practical reality and we are now able to routinely measure large cohorts of single cell proteomes to reveal new biological information. The Evosep One has been pivotal to scale this robustly to thousands of real single cell measurements, making it an ideal and standardized platform for future biomarker discovery work.”,
Professor Matthias Mann, Max-Planck Institute of Biochemistry,
Proteomics and Signal Transduction
TRUE SINGLE-CELL PROTEOMICS
One of the greatest goals of single-cell proteomics is to reach the needed sensitivity and maintain the performance throughout the lifetime of a project.
Andreas-David Brunner from the Mann group at the Max Planck Institute of Biochemistry has developed a robust workflow combining miniaturized sample preparation, the Whisper100 40SPD beta method and a novel trapped ion mobility mass spectrometer.
This workflow allowed the accurate quantification of proteomes and their changes up to a depth of 1,300 proteins when loading single, FACS-isolated cells.