Whisper 40SpD BETA

This EVOSEP+ method is the ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell and phospoproteomics analysis. 

SINGLE CELL PROTEOMICS IS A ROBUST STANDARDIZED APPLICATION

Whisper 40SPD beta is the fastest method in a series of methods developed with Whisper Flow Technology. It has a gradient flow of 100 nl/min with a 30 minutes method duration – delivered robustly by standard Evosep One hardware.

The method is ideal for ultra-sensitive applications, such as single cell proteomics, where one of the main challenges is to maintain sensitivity and robustness throughout the workflow.

Ultra sensitive method optimized for single cell analysis

The Whisper 40SPD Beta us design for our EV1112 column and EV1111 fused silica emitter. It runs with a gradient flow of 100 nl/min and provides sharp peaks with a median FWHM of 4.8 seconds.

It is part of our dynamic beta environment and updates may be implemented for improved performance. 

Webinar: Whisper is here

Learn more about the advantages of the pioneering Whisper™ flow technology in our webinar hosted by Nicolai Bach, Head of Applications at Evosep, from December 16th 2021. 

THOUSANDS OF UNINTERRUPTED RUNS WITH THE SAME COLUMN AND EMITTER

Robustness and reproducibility is important for scalable operations. The Whisper100 40SPD beta method is therefore carefully designed for our EV1112 column and EV1111 fused silica emitter.

This combination is extremely robust as only 20 bars increase in system backpressure was observed after thousands of runs as well as a reproducible flow profile.

IT’S ALL IN THE EVOTIP

The Evotip, a disposable trap column, is the core of our technology. The Evotip serves as an integrated purification step, where contaminants are left on the tip, and analytically relevant peptides are eluted.

This minimizes cross-contamination and extends column lifetime. The Evotip has proven to be extraordinary important for ultra-sensitive analysis, as very small sample amounts can be loaded in higher volumes.

Additionally, loaded Evotips can be stored cold prior to analysis.

Get well started with Whisper

We are proud to introduce our new Whisper methods and would like you to get started well. Here you can find a step-by-step guide describing how to connect your column and emitter to obtain optimal peak shapes and thereby the best chromatographic performance.

Download guide

Webinar: The launch in 2020

The Whisper™ flow technology was first launched on December 15th 2020. In this webinar, hosted by Nicolai Bache, Head of Applications at Evosep, you can learn more about the principles and advantages of the pioneering Whisper™ flow technology.

Additionally, Andreas-David Brunner from the Mann group at the Max Planck Institute of Biochemistry presents how he has implemented a nearly loss-less sample preparation strategy with the Whisper methods and diaPASEF on the timsTOF SCP mass spectrometer to analyze single-cell proteomics.

Sign up to our WHisper (beta) user group

Whisper is part of our dynamic beta environment, where updates are to be expected. Sign up here to get notified with relevant information.

explore all available methods with evosep+

Find the method fitting your research need and get inspired with data from Evosep users.

The extended method 

EVOSEP+ method

Maximum proteome coverage in a single-shot analysis with extended gradient duration.

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The High organic method

EVOSEP+ method

This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.

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Whisper
40SPD beta

EVOSEP+ method

The ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell analysis.

LEARN MORE

Whisper
20SPD beta

EVOSEP+ method

A longer method for sensitive nanoflow applications.

LEARN MORE

DOWNLOAD OUR MATERIAL

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ULTRA-SENSITIVE AND STABLE PERFORMANCE WITH WHIsPER FLOW TECHNOLOGY
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Whisper™ step-by-step guide
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Column ovens for Evosep performance columns
BROCHURE
columns and emmitters overview
tech note
EVOSEP ONE: A GRADIENT OFF-SET FOCUSING UHPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT PROTEOMICS

Want to know more?

Our team is ready to help you find the method fitting your research need. Fill out our contact form and we will get back to you as soon as possible