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AN AUTOMATED OPENTRONS LOADING PROTOCOL FOR SAMPLE LOADING OF
EVOTIP PURE
A complete, automated end-to-end sample loading protocol for the Opentrons OT-2 makes use of a layered sandwich approach with defined airgaps between the layers. This sandwich is pushed through the Evotip with the OT-2 pipette for 100 seconds leaving the Evotip ready for injection on the Evosep One.
The automated sample loading protocol relies on a defined sample volume of 20 µl and solvent A volumes of 15 µl and 150 µl respectively to keep the sandwich intact through loading.
The protocol has been converted into an easy-to-use HTML form, that generates a complete python script for use in the Opentrons app, allowing the user to load from 8 to 288 Evotips in a single run.
An Evotip Loading kit (OT-2) (EV1144) is required to run the protocol as well as two pipettes; an Opentrons 8-channel P20 pipette and an Opentrons 8-channel P300 pipette.
An Opentrons OT-2 protein on-bead digestion and evotip loading protocol
An end-to-end workflow encompassing cell lysate or plasma to digest-loaded Evotip has been developed. The method is automated on Opentrons OT-2 liquid handling system covering steps such as aggregation-based protein capture on magnetic beads, clean-up and digestion as well as seamless Evotip loading based on layered sandwich approach.
The protocol has been converted into an easy-to-use HTML form, that generates a complete python script to use in the Opentrons app. It allows the user to process from 8 to 192 samples in a single run.
An Evotip Loading kit (OT-2) (EV1144) and Evotip Pure (EV-2011) is required to run the protocol as well as an Opentrons 8-channel P300 pipette and a Magnetic module (GEN2).
Protocols
Opentrons ot-2 Evotip Loading protocol
Opentrons OT-2 Evotip loading protocol allowing you to generate a protocol that suits your needs. The protocol uses the Opentrons 8-channel P20 and P300 pipettes to load eight Evotip Pure at the time and up to 288 Evotip Pure per protocol run.
Generate protocol file and import to Opentrons app.
Opentrons OT-2 Protein on-bead digestion and evotip loading protocol
Opentrons OT-2 Protein on-bead digestion and Evotip loading protocol enables you to generate a complete end-to-end sample preparation protocol for cell lysates, that carries out digestion and Evotip loading in a single hands-free process. The protocol uses the Opentrons 8-channel P300 pipettes and handles up to 192 samples per protocol run.
Generate protocol file and import to Opentrons app.
Step-by-Step Guide
Opentrons ot-2 Evotip Loading protocol
Download the step-by-step guide on how to use the Opentrons OT-2 Evotip Loading Protocol.
See video guides below as well.
Opentrons OT-2 Protein on-bead digestion and Evotip Loading Protocol
Download the step-by-step guide on how to use the Opentrons OT-2 Protein On-Bead Digestion and Evotip Loading Protocol.
Application notes
A complete, automated Opentrons OT-2 loading protocol for simplified sample loading of Evotip Pure
App note describing automatic loading protocol for loading of Evotips.
End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2
App note describing digestion and automatic loading protocol for loading of Evotips.
