Documentation
Here you can find all the latest documentation for the Evosep Instruments.
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Technology
Evosep eno – A standardized separation tool for proteomics
Evosep Eno is an easy-to-use, robust and reliable chromatographic solution for high performance and standardized proteomics, enabling seamless integration between sample preparation and LC-MS analysis in a sustainable and cost-efficient manner.
Evosep One – A standardized separation tool for proteomics
Evosep aims to improve quality of life and patient care by radically innovating protein-based clinical diagnostics. This brochure introduces our instrument Evosep One.
Columns and emitters overview – Evosep One
Learn more about our range of columns and emitters in the brochure and visit our online shop to place your order.
Whisper zoom technology
Here you can find a step-by-step guide describing how to change the tubing on the Evosep One to obtain optimal peak shapes and thereby the best chromatographic performance.
LEGACY – Whisper Flow technology
Here you can find a step-by-step guide describing how to connect your column and emitter to obtain optimal peak shapes and thereby the best chromatographic performance.
Manuals
Evosep eno user Manual – english
The Evosep Eno User Manual covers information and instructions on the setup and use of the Evosep Eno. It contains guidance on maintenance recommendations as well as support, service and warranty policies.
Evosep eno user manual – french
Le manuel utilisateur Evosep Eno couvre les informations et les instructions sur l’installation et l’utilisation de l’Evosep Eno. Il contient des conseils sur les recommandations de maintenance ainsi que sur les politiques de support, de service et de garantie.
Evosep eno Advanced user guide
This Advanced User Guide is designed as a companion to the User Manual, Quick Start Guides and Software Installation Guides to provide guidance on more advanced Evosep Eno functions, connections to specific CDS, MS ionization sources and troubleshooting.
Evosep POD user manual
The Evosep Pod User Manual covers information and instructions on the setup and use of the Evosep Pod. It contains guidance on support, service and warranty policies.
Evosep One user guide
The Evosep One user guide covers information regarding the installation, setup and use of Evosep One. It contains troubleshooting guidance, routine maintenance recommendations and support, service and warranty policies.
Protocols
SAMPLE LOADING PROTOCOL FOR EVOTIPS
The standard loading protocol for loading samples contains step-by-step descriptions on how to load samples on Evotips including required material, tips for best practice.
A COMPLETE, AUTOMATED OPENTRONS OT-2 LOADING PROTOCOL FOR SIMPLIFIED SAMPLE LOADING OF EVOTIP PURE
App note describing automatic loading protocol for loading of Evotips.
END-TO-END, FULLY AUTOMATED DIGESTION PROTOCOL AND EVOTIP PURE WORKFLOW ON THE OPENTRONS OT-2
App note describing digestion and automatic loading protocol for loading of Evotips.
Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo
App note describing fully-automated AssayMAP Bravo protocol for loading of Evotips
Quick guide
Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads.
Evotip brochure
Evotip Pure™ is a new generation Evotip developed for high performance. To ensure quality, we have internalized and refined all critical manufacturing and carefully chosen the chemistry.
Application notes
Evosep Eno
Reproducible, inter-laboratory, scalable and routine LC-MS based proteomics – with the Evosep Eno
The Evosep Eno, designed with applied proteomics in mind, offers exceptional system stability, standardization, unmatched performance and ease-of-use.
Evosep Eno – high sensitivity and quantitative accuracy for plasma proteome analysis at scale
Recent advancements in sample preparation and LC-MS technology are enabling deeper coverage of the plasma proteome, with the potential to detect over 7,000 proteins in single-shot analyses.
Evosep Eno – new standards for routine, scalable, and high-performance LC-MS based proteomics
Mass spectrometry (MS)-based proteomics is rapidly evolving, offering a powerful suite of technologies with the potential to revolutionize healthcare and enable precision medicine.
Biological insights at scale with Evosep Eno – 500 samples per day throughput
The robustness and high performance of the analytical pipeline enabled by the Evosep Eno, allow researchers to scale studies to larger cohorts and expand screening conditions to gain deeper biological insight.
Standardized methods – Evosep One
Towards a Standardized Omics Platform with the 500 samples per day method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Towards a Standardized Omics Platform with the 300 samples per day method
Very high peak capacity per minute for high-throughput analysis.
Towards a Standardized Omics Platform with the 200 samples per day method
Excellent sequencing speed with most peptides per minute among our standard methods.
Towards a Standardized Omics Platform with the 100 samples per day method
A great “in-between” method with run-to-run reproducibility of just one second.
Towards a Standardized Omics Platform with the 60 samples per day method
The most popular method with an excellent balance of throughput and depth.
Towards a Standardized Omics Platform with the 30 samples per day method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
Specialized methods – Evosep One
Whisper Zoom drives scalable workflows for high-sensitivity applications through standardization and ease-of-use
Robust and scalable throughput with up to 120 samples per day.
A specialized method for high sensitivity with the whisper zoom 120 Samples per day method
The Whisper Zoom 120 SPD method is designed to push the boundaries of scalability for high-sensitivity applications.
A specialized method for high sensitivity with the whisper zoom 80 Samples per day method
The Whisper Zoom 80 SPD method strikes the perfect balance between throughput and performance for routine applications.
A specialized method for high sensitivity with the whisper zoom 40 Samples per day method
The Whisper Zoom 40 SPD method offers high performance with the sensitivity needed to miniaturize your workflows
A specialized method for high sensitivity with the whisper zoom 20 Samples per day method
The Whisper Zoom 20 SPD method excels in applications requiring both sensitivity and extended separation.
A specialized method for maximum proteome coverage with the Extended Method
Maximum proteome coverage in a single-shot analysis with extended gradient duration.
A specialized separation method for hydrophobic targets with the High Organic Method
Enables small-molecules analysis with significantly more hydrophobic targets.
Specialized methods – legacy
A specialized method using Whisper Flow technology with a throughput of 80 samples per day
A high-throughput method for scaling single cell analysis.
A specialized method using Whisper Flow technology with a throughput of 40 samples per day
Ultra-sensitive method ideal for single-cell analysis.
A specialized method using Whisper Flow technology with a throughput of 20 samples per day
A longer method for robust and sensitive applications.
Other
The proteomics dilemma – high throughput analysis versus proteome depth
The Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.
Comprehensive proteomes with 8000 proteins identified with dia-PASEF using the 30 samples per day method
Data-independent acquisition based proteomics measurements in combination with the Evosep One for a powerful workflow for deep and accurate mapping of proteomes across large sample cohorts.
Fast and reproducible phosphoproteomics usign MagResyn Amine and Ti-IMAC HP magnetic beads and the Evosep One
Read this application note to learn about the automated workflows for sample preparation developed by the Olsen group in collaboration with Resyn Biosciences. Protein digestion and phosphoenrichment is fully automated for up to 96 samples in parallel with the Kingfisher Flex robot from Thermo Scientific.
Fast confirmatory analysis of rhuepos in plasma for horseracing doping control
This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.
Case studies
Next generation LC-MS based proteomics – a new era of speed, scale and robustness
Liquid chromatography-mass spectrometry (LC-MS) is a cornerstone of modern proteomics research, revealing the intricate interactions of proteins driving cellular function and disease.
Unleashing the true power of DIA/SWATH data acquisition with short gradients – Rapid profiling of pre-clinical models and proteome dynamics
Achieving sufficient statistical power for the analysis of a comprehensive set of target proteins requires processing of a large number of samples. With finite resources, researchers face a difficult choice: should they aim at deeper proteomic coverage or higher sample throughput?
A fleet of Evosep One instruments supports a proteomics factory at Rapid Novor
This Case Study provides a glimpse behind the scenes at Rapid Novor, a core facility equipped with eight Evosep One instruments running 24/7. Read how instrument-to-instrument standardization played a vital role in the creation of de novo sequencing workflows in the laboratory.
