Documentation

Here you can find all the latest documentation for the Evosep Eno. 

Have a technical issue? Contact support or access online troubleshooting

Technology

Evosep eno – A standardized separation tool for proteomics

Evosep Eno is an easy-to-use, robust and reliable chromatographic solution for high performance and standardized proteomics, enabling seamless integration between sample preparation and LC-MS analysis in a sustainable and cost-efficient manner. 

Manuals

Evosep eno user Manual – english

The Evosep Eno User Manual covers information and instructions on the setup and use of the Evosep Eno. It contains guidance on maintenance recommendations as well as support, service and warranty policies. 

Evosep eno user manual – french

Le manuel utilisateur Evosep Eno couvre les informations et les instructions sur l’installation et l’utilisation de l’Evosep Eno. Il contient des conseils sur les recommandations de maintenance ainsi que sur les politiques de support, de service et de garantie. 

Evosep eno Advanced user guide

This Advanced User Guide is designed as a companion to the User Manual, Quick Start Guides and Software Installation Guides to provide guidance on more advanced Evosep Eno functions, connections to specific CDS, MS ionization sources and troubleshooting. 

Evosep POD user manual

The Evosep Pod User Manual covers information and instructions on the setup and use of the Evosep Pod. It contains guidance on support, service and warranty policies. 

Evosep POD Troubleshooting guide

This troubleshooting guide is a companion document to the Evosep Pod User Manual (UM-002). The user manual contains instructions for Evosep Pod use. The troubleshooting guide lists potential failure modes with their possible causes and resolutions.

Manuals – Software drivers

Quick Start Guide – Chronos

This Evosep Eno Quick Start Guide covers information and instructions on the setup and use of the Chronos (and Xcalibur) software. 

Software Installation Guide – Chronos

This installation guide supports the installation of Evosep Eno driver when used with Axel Semrau® CHRONOS version 5.2.17.

Quick Start Guide – Hystar

This Evosep Eno Quick Start Guide covers information and instructions on the setup and use of the Compass HyStar software. 

Software Installation Guide – Hystar

This installation guide supports the installation of Evosep Eno driver when used with Bruker Compass® HyStar. 

Quick Start Guide – SCIEX OS

This Evosep Eno Quick Start Guide covers information and instructions on the setup and use of the SCIEX OS software. 

Software Installation Guide – SCIEX OS

This installation guide supports the installation of Evosep Eno driver when used with SCIEX OS. 

Protocols

SAMPLE LOADING PROTOCOL FOR EVOTIPS

The standard loading protocol for loading samples contains step-by-step descriptions on how to load samples on Evotips including required material, tips for best practice.

A COMPLETE, AUTOMATED OPENTRONS OT-2 LOADING PROTOCOL FOR SIMPLIFIED SAMPLE LOADING OF EVOTIP PURE

App note describing automatic loading protocol for loading of Evotips.

END-TO-END, FULLY AUTOMATED DIGESTION PROTOCOL AND EVOTIP PURE WORKFLOW ON THE OPENTRONS OT-2

App note describing digestion and automatic loading protocol for loading of Evotips.

Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo

App note describing fully-automated AssayMAP Bravo protocol for loading of Evotips

A semi-automated Assaymap Bravo protocol for robust evotip loading and high-throughput analysis on the Evosep One

App note of semi-automated AssayMAP Bravo protocol for loading of Evotips

Quick guide

Using Evotips results in simplified workflows as integrating elution with liquid chromatography removes several sample handling steps as well as reduces injection cycle overheads.

Evotip brochure

Evotip Pure™ is a new generation Evotip developed for high performance. To ensure quality, we have internalized and refined all critical manufacturing and carefully chosen the chemistry.

Application notes

Reproducible, inter-laboratory, scalable and routine LC-MS based proteomics – with the Evosep Eno

The Evosep Eno, designed with applied proteomics in mind, offers exceptional system stability, standardization, unmatched performance and ease-of-use.

Evosep Eno – high sensitivity and quantitative accuracy for plasma proteome analysis at scale

Recent advancements in sample preparation and LC-MS technology are enabling deeper coverage of the plasma proteome, with the potential to detect over 7,000 proteins in single-shot analyses.

Evosep Eno – new standards for routine, scalable, and high-performance LC-MS based proteomics

Mass spectrometry (MS)-based proteomics is rapidly evolving, offering a powerful suite of technologies with the potential to revolutionize healthcare and enable precision medicine.

Biological insights at scale with Evosep Eno – 500 samples per day throughput

The robustness and high performance of the analytical pipeline enabled by the Evosep Eno, allow researchers to scale studies to larger cohorts and expand screening conditions to gain deeper biological insight.

Case studies

Next generation LC-MS based proteomics – a new era of speed, scale and robustness

Liquid chromatography-mass spectrometry (LC-MS) is a cornerstone of modern proteomics research, revealing the intricate interactions of proteins driving cellular function and disease.