Drug Discovery

As proteins are the targets for most drugs, proteomics is important to study novel targets and understand disease mechanisms and toxicity.

Explore, how the Evosep One technology is a valuable tool for high-throughput and reliable drug discovery.

High-throughput drug discovery with the Evosep One 

Drug discovery is the process through which potential new medicines are identified. It involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.

The complexity in drug development has increased over the past 40 years, requiring preclinical testing, investigational new drug applications, and completed clinical testing before marketing approval from the FDA.
The overarching goal is to bring more efficient and safer treatments to the patients as quickly as possible after a thorough medical evaluation.

High-throughput and sensitive analysis on the Evosep One is a good fit when developing new drug applications or biologics license applications.

User talk

TARGETED ASSAY DEVELOPMENT FOR QUANTIFYING PROTEIN HALF-LIFE

In this user talk, Thomas Angel from the Exploratory Biomarker Assay group at GlaxoSmithKline present their work on in vivo metabolic labeling and protein turnover.

They make use of fast gradients on the Evosep One coupled to high resolution MS to enable targeted analysis of peptides from bespoke targets, facilitating quantification of label incorporation and determining protein fractional synthesis across multiple mouse tissues.

Watch presentation on YouTube

Robust and Reproducible Protein Quantification in Plasma 

At Pelago Biosciences, they have stablished the Cellular Thermal Shift Assay (CETSA) in a high-throughput drug discovery pipeline with the Evosep One, enabling them to analyze a large number of compounds and unravel common or distinct pathways activation.

In this publication, they present a deep and comprehensive profiling of Staurosporine induced proteome thermal stability alteration utilizing different experiment layouts.

Ultimately, they collected data from 14 individual replicates of Staurosporine-treated K562 lysates, which allowed them to quantify more than 7500 proteins.

From this comprehensive dataset, they were able to detect 293 proteins with concentration dependent changes in thermal stability – 169 stabilized proteins (117 kinases – in blue) and 63 (11 kinases – in red) destabilized proteins.

Read full publication

EVOSEP WEBINAR

MEET OUR USERS and their research using targeted workflows

High-throughput drug discovery with the Evosep One

THU September 30  I  4:00 pm CEST

In this webinar we will look into how the Evosep One with high-throughput and sensitivity is a good fit when developing new drug applications or biologics license applications.

More research with Drug Discovery

Here you can see publications available on drug discovery featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here

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find all publications, product brochures and presentations with Evosep One

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application note
HOW FAR WILL YOU GO? THE FIVE STANDARD METHODS FOR THE EVOSEP ONE
application note
The proteomics dilemma – High throughput analysis versus proteome depth
case study
PAVING THE WAY TO CLINICAL PROTEOMICS
brochure
EVOSEP ONE: A Standardized Separation
Tool for Clinical Omics