Elevate Efficiency

The power of automation

Automation streamlines labor-intensive processes, significantly reducing manual intervention and human error. By automating tasks such as sample preparation, digestion, fractionation, and enrichment, you can process larger sample cohorts, optimize throughput, and accelerate data generation.

Fast

Cost-efficient

Reproducible

Scalable

convenient

easy

The new standard for sample preparation

The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instrument per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation.

To overcome this bottleneck, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is the central component of a modular automation strategy that allows for addition of functional modules such as digestion and enrichment steps.

fast and efficient, automated sample preparation protocol on the Opentrons OT-2

Our solution provides you with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2 (OT-2) utilizing protein aggregation capture (PAC) on magnetic microparticles in an end-to-end standardized hands-offworkflow, starting from protein lysate to peptide loaded on Evotips.

The Evotip is an essential component, where the entire sample is efficiently loaded in a high volume just after preparation, minimizing losses by removing unnecessary sample handling steps. Furthermore, the Evotip serves as a highly efficient, temporary storage device, allowing for complete sample handling on the robot and transfer of the prepared Evotips to the LC/MS instrumentation, once available or whenever convenient.

From digestion to discovery with automation

The success of proteomics heavily relies on simple and reproducible workflows for identification and quantification of proteins.

Efficient digestion is a fundamental component in such a workflow and by implementing an automated digestion protocol, you can achieve deep proteome coverage reproducibly and cost-efficiently. Important when analyzing large sample cohorts.

Automation is a powerful tool that revolutionizes the field of proteomics by enhancing the digestion process. It ensures the reliability, speed, and cost-effectiveness of sample preparation, making large-scale proteomic studies more accessible and offering deeper insights into biological systems.

Let’s get in touch

Get started with automated
sample preparation

We want to make it easy for you to get started. Fill in you information to get contacted by and Evosep representative. We would be happy to set up a meeting to learn more about your current needs.

Elevate efficiency

Automation accelerates proteomics, optimizing throughput and reducing costs, making it ideal for labs of all experience levels. With automation you can streamline the entire sample preparation process, especially for large sample volumes or when sensitivity and standardization are crucial.

Free up more time for new discoveries, optimize resources, and tackle larger sample cohorts with ease. Our protocol unleashed efficiency, empowering you to unlock the full potential of your proteomic research.

fast
Optimize throughput, and accelerate data generation

To scale up proteomics, speed is of the essence. Automation has the power to turbocharge data generation and with our loading protocol you are all set for rapid processing, significantly reducing the time required for sample preparation.

Reproducible
consistency and reproducibility across samples

With automation you can minimize the risk of human error, resulting in highly reproducible results. Whether you’re analyzing ten or a hundred samples, you can trust that your data will be consistent, facilitating robust scientific conclusions.

convenient
It’s all in the evotip

With automation comes convenience. The Evotip speeds up your sample loading, reduces carry-over and is a significant contribution to why proteomics has become so easy and routine.

scalable
accommodate larger sample cohorts

The workflow should be scalable and easy to use in any lab. Our protocol is designed with scalability in mind and you can seamlessly process hundreds of samples in a single workday.

Cost-efficient
Automation offers a cost-effective alternative

By optimizing sample usage and reducing reagent consumption, our system streamlines processes while minimizing waste, automation can substantially reduce the overall cost of proteomic analyses.

Easy
One size fits all with standardization

Minimizes the complexity with our user-friendly design. This means that you can easily integrate our protocol into your existing workflows, regardless of your level of expertise.

WEBINAR: ELEVATE EFFICIENCY – THE POWER OF AUTOMATION

This webinar is your guide to get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2. By adopting full automation, you can gain precise control over protocols, ensuring reproducibility across samples and experiments. This minimizes batch effects, resulting in more reliable data for analysis and interpretation.

NOVEMBER 9TH  I  4:00 PM CET  I 10:00 AM EST

More research using an automated sample prep workflow

Here you can see publications available using an automated sample preparation workflow featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
MassSpecPreppy—An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications2023

Read publication here

Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel, , , 2023

Read publication here

End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-22023The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instru- ment per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation. To address this, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is …,

Download application note here

Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo2023Automation and robotics have revolutionized the field of proteomics, offering solutions to the growing need for streamlined workflows and efficient sample processing. In this context, automated loading of Evotips has emerged as a pivotal advancement in sample preparation workflows. The Evotip can now be loaded using an innovative and fully automated strategy with the Agilent …, ,

Download application note here

A complete and automated sample preparation strategy for high-throughput and standardized proteomics, applied to a clinical cohort of patient plasma samples2022,

Read poster here

Automated sample prep workflows2021In this webinar, we will focus on automated samples prep workflows. Users will share how they have standardized their sample prep workflows and discuss the advantages of combining automated sample prep workflows with the Evosep One.

Watch webinar

Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures, , , , , 2021In this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1 inhibition in T-cell acute lymphoblastic leukemia.,

Read publication here

Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids, , , 2021In this seminar from the Canadian National Proteomics Network (CNPN) meeting 2021, Angela McArdle presents a standardized workflow for precise high-throughput proteomics of blood biofluids. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood.

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Learn more

High-resolution longitudinal serum proteome trajectories in COVID-19 reveal patients-specific seroconversion, , 2021This publication from OmicEra Diagnostics, describes alterations of the serum proteome during COVID-19 using a scalable plasma proteome profiling workflow. It comprises the most detailed longitudinal protein trajectories during hospitalization, based on one of the largest MS-based body fluid proteomics efforts with a total of 720 plasma serum samples.

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho., ,

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Evosep application note for AssayMAP bravo protocol for Evotip
Application Note
End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2
Evosep application note for AssayMAP bravo protocol for Evotip
Application Note
Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo
application note
Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
brochure
EVOSEP ONE: A Standardized Separation
Tool for Clinical Omics
application note
A complete, automated Opentrons OT-2 loading protocol
for simplified sample loading of Evotip Pure