Elevate Efficiency
The power of automation
Automation streamlines labor-intensive processes, significantly reducing manual intervention and human error. By automating tasks such as sample preparation, digestion, fractionation, and enrichment, you can process larger sample cohorts, optimize throughput, and accelerate data generation.
Fast
Cost-efficient
Reproducible
Scalable
convenient
easy
The new standard for sample preparation
The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instrument per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation.
To overcome this bottleneck, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is the central component of a modular automation strategy that allows for addition of functional modules such as digestion and enrichment steps.
fast and efficient, automated sample preparation protocol on the Opentrons OT-2
Our solution provides you with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2 (OT-2) utilizing protein aggregation capture (PAC) on magnetic microparticles in an end-to-end standardized hands-offworkflow, starting from protein lysate to peptide loaded on Evotips.
The Evotip is an essential component, where the entire sample is efficiently loaded in a high volume just after preparation, minimizing losses by removing unnecessary sample handling steps. Furthermore, the Evotip serves as a highly efficient, temporary storage device, allowing for complete sample handling on the robot and transfer of the prepared Evotips to the LC/MS instrumentation, once available or whenever convenient.
From digestion to discovery with automation
The success of proteomics heavily relies on simple and reproducible workflows for identification and quantification of proteins.
Efficient digestion is a fundamental component in such a workflow and by implementing an automated digestion protocol, you can achieve deep proteome coverage reproducibly and cost-efficiently. Important when analyzing large sample cohorts.
Automation is a powerful tool that revolutionizes the field of proteomics by enhancing the digestion process. It ensures the reliability, speed, and cost-effectiveness of sample preparation, making large-scale proteomic studies more accessible and offering deeper insights into biological systems.
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Get started with automated
sample preparation
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Elevate efficiency
Automation accelerates proteomics, optimizing throughput and reducing costs, making it ideal for labs of all experience levels. With automation you can streamline the entire sample preparation process, especially for large sample volumes or when sensitivity and standardization are crucial.
Free up more time for new discoveries, optimize resources, and tackle larger sample cohorts with ease. Our protocol unleashed efficiency, empowering you to unlock the full potential of your proteomic research.
fast
Optimize throughput, and accelerate data generation
Reproducible
consistency and reproducibility across samples
With automation you can minimize the risk of human error, resulting in highly reproducible results. Whether you’re analyzing ten or a hundred samples, you can trust that your data will be consistent, facilitating robust scientific conclusions.
convenient
It’s all in the evotip
With automation comes convenience. The Evotip speeds up your sample loading, reduces carry-over and is a significant contribution to why proteomics has become so easy and routine.
scalable
accommodate larger sample cohorts
Cost-efficient
Automation offers a cost-effective alternative
By optimizing sample usage and reducing reagent consumption, our system streamlines processes while minimizing waste, automation can substantially reduce the overall cost of proteomic analyses.
Easy
One size fits all with standardization
Minimizes the complexity with our user-friendly design. This means that you can easily integrate our protocol into your existing workflows, regardless of your level of expertise.
WEBINAR: ELEVATE EFFICIENCY – THE POWER OF AUTOMATION
This webinar is your guide to get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2. By adopting full automation, you can gain precise control over protocols, ensuring reproducibility across samples and experiments. This minimizes batch effects, resulting in more reliable data for analysis and interpretation.
NOVEMBER 9TH Â I Â 4:00 PM CET Â I 10:00 AM EST
More research using an automated sample prep workflow
Here you can see publications available using an automated sample preparation workflow featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here
| Title | Subject | Material | Year | Summary | Institute | Evosep method | MS instrumentation | Learn More |
|---|---|---|---|---|---|---|---|---|
| Using a cost-efficient and scalable plasma proteomics pipeline to analyze more than 1,100 patient samples | Automation, Plasma Proteomics | Application note | 2025 | 200 SPD | Bruker timsTOF HT | |||
| Scalability for high-throughput proteomics – Evotip Pure integration with the Biomek i5 liquid handler | Automation | Application note | 2024 | With the development of faster and more sensitive mass spectrometers, proteomics has become an essential tool across diverse applications in both academia and industry. As proteomics studies become larger and analyses become more routine, the demand for standardized and scalable solutions is increas- ing. To address this need, we present four fully automated workflows developed … | 200 SPD, 500 SPD | Bruker timsTOF HT | ||
| Efficient proteomics with an automated sample preparation strategy leveraged by Evotip Pure and the AssayMAP Bravo | Automation | Application note | 2024 | Proteomics is undergoing a significant shift and rapidly evolving towards more efficient and user-friendly technologies that can handle higher quantities of samples. The Evosep One, combinedwith improvements in high-end mass spectrometry, has played a vital role in transforming this field, making it possible to analyze hundreds of proteomes daily. Consequently, the bottleneck in high-throughput proteomics … | 100 SPD, 500 SPD, Whisper Zoom 40 SPD, Whisper Zoom 80 SPD | Bruker timsTOF HT | ||
| Unlocking the plasma proteome with Evotip Pure based, standardized and scalable workflows | Automation | Application note | 2024 | Plasma stands out as one of the most readily accessible biofluids, with an estimated pres- ence of over 10,000 distinct proteins. A significant portion of these are released from organs and tissues. The ability to measure such proteins in a robust manner holds tremendous potential for advancing next-generation multiplexed biomarker discovery. However, methods must be … | 100 SPD | Thermo Orbitrap Astral | ||
| Evotip Pure brings innovative sample storage and introduction to high-sensitivity and scalable LC-MS workflows | Automation | Application note | 2024 | The Evotip Pure technology has emerged as a key component of proteomics workflows enabling seamless integration of upstream sample prepa- ration with LC-MS analysis by reducing sample handling steps and thereby efficiently minimizing sample losses to plasticware, which significantly improves recovery and reproducibility. These properties are even more critical in single-cell proteomics (SCP), a rapidly … | ||||
| MassSpecPreppy—An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications | Automation | Publication | 2023 | 30 SPD | Bruker timsTOF Pro 2 | |||
| Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel | Automation, DIA, Single Cell Proteomics | Journal article | 2023 | Whisper 40 SPD | Bruker timsTOF Pro | |||
| End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2 | Automation | Application note | 2023 | The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instru- ment per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation. To address this, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is … | 100 SPD, Whisper 40 SPD | Bruker timsTOF Pro 2 | ||
| Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo | Automation | Application note | 2023 | Automation and robotics have revolutionized the field of proteomics, offering solutions to the growing need for streamlined workflows and efficient sample processing. In this context, automated loading of Evotips has emerged as a pivotal advancement in sample preparation workflows. The Evotip can now be loaded using an innovative and fully automated strategy with the Agilent … | 100 SPD, Whisper 40 SPD | Bruker timsTOF Pro 2, SCIEX ZenoTOF 7600 | ||
| A complete and automated sample preparation strategy for high-throughput and standardized proteomics, applied to a clinical cohort of patient plasma samples | Automation | Poster | 2022 | 500 SPD, Whisper 40 SPD | Bruker timsTOF Pro | |||
| Automated sample prep workflows | Automation | Webinar | 2021 | In this webinar, we will focus on automated samples prep workflows. Users will share how they have standardized their sample prep workflows and discuss the advantages of combining automated sample prep workflows with the Evosep One. | ||||
| Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures | Automation, DDA, DIA, T-ALL | Publication | 2021 | In this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1 inhibition in T-cell acute lymphoblastic leukemia. | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids | Automation, Clinical research, DIA | Webinar | 2021 | In this seminar from the Canadian National Proteomics Network (CNPN) meeting 2021, Angela McArdle presents a standardized workflow for precise high-throughput proteomics of blood biofluids. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood. | 60 SPD | Thermo Orbitrap Exploris 480 | ||
| High-resolution longitudinal serum proteome trajectories in COVID-19 reveal patients-specific seroconversion | Automation, Clinical research, Covid-19 | Publication | 2021 | This publication from OmicEra Diagnostics, describes alterations of the serum proteome during COVID-19 using a scalable plasma proteome profiling workflow. It comprises the most detailed longitudinal protein trajectories during hospitalization, based on one of the largest MS-based body fluid proteomics efforts with a total of 720 plasma serum samples. | 60 SPD | Bruker timsTOF Pro | ||
| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, Technology | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho. | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 |
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Application Note
End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2
Application Note
Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo
application note
Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
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EVOSEP ONE: A Standardized Separation
Tool for Clinical Omics
