Find answers to the most asked questions below.

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Evosep One

We do not recommend using other type of columns. Evosep One methods are specifically optimized for Evosep One columns. You can find the full overview of our columns here.

No, the Evosep One is accurately designed for a perfect low-pressure, leak-free fit with Evotips. Using other pipette tips will void instrument warranty.

No, once the sample has been eluted from the Evotip, the plastic will be slightly deformed and cannot be used again. Doing so, will void instrument warranty.

No, the methods are fixed and designed for optimal performance with our columns. You can find the full overview here

We recommend loading 20ul of sample, but volume can be changed if the loading protocol is adapted accordingly (higher volume => Longer centrifugation time, lower volume => shorter centrifugation time)


No, Evosep One methods are optimized using 100% water as solvent A and 100% Acetonitrile as Solvent B (both with 0.1% formic acid, both in LC/MS grade quality).

No, direct infusion is not possible with the Evosep One system.

The stationary phase of the Evotip is C18.

No, Evotips are only available with C18 material.

It varies from method to method. You can find the overview of the flow rates in the standard methods here

In Windows 10 go to the menu called “App & features” and click the Evosep One Chronos Plugin icon. On older Windows versions the info can be found under programs & features.

You can find the overview of the five standard methods here. Additionally, there are a number of Evosep+ methods for specialized applications. You can find the overview of these here.

You can find the latest software releases here. You can access the Evosep One product information, manuals and application notes here.

Our methods are designed to give the best chromatographic performance with our matched columns. You can find the latest method specific peak performance in our applications notes here.

Yes. By using the soak adaptor with part no EV1068 and the Thermo Scientific Nunc deep well plate with part no. 260251 it is possible to collect up to 500 µl from each tip.