Advancements in Technology Setting new Standards for Scalable Single-Cell Proteomics: Identification of Over 5,000 Proteins in a Single HeLa Cell

This manuscript, authored by the Olsen group at the Novo Nordisk Foundation Center for Protein Research, describes technological advancements that set a new standard for scalable single-cell proteomics. By comprehensively addressing the entire workflow, the first commercially available solution for single-cell proteomics has become a reality, enabling the identification of more than 5,000 proteins from a single cell.