Evosep One facilitates rapid and comprehensive maps of the subcellular proteome and phospho-proteome dynamics
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level.
Breakthrough paper by the Mann group demonstrates trat true single-cell proteomics is now a reality to address important questions in cell biology
Single-cell technologies are revolutionizing biology but have so far mainly been limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS)- based proteomics would thus be highly valuable and complementary.