EVOSEP ONE: EV1000
Evosep One is an easy-to-use, robust and reliable chromatographic solution for high performance and standardized proteomics, enabling seamless workflow integration.Next generation insights with Evosep One
LC-MS based proteomics is not just about individual technologies. It covers the entire workflow from sample preparation to data acquisition – ensuring sensitivity, reliability, and speed. The Evosep One is a standardized platform designed for robustness and high throughput, integrating sample preparation with LC-MS analysis to streamline and optimize your entire workflow – not just individual performance metrics.
Maximum reliability: Built with durable components to ensure consistent, long-term performance.
Minimized downtime: Designed to keep your research on track with fewer interruptions and more uptime.
Patented approach: Innovative sample introduction and separation technology for seamless, efficient operation (US20200041467B1,
US20190250130B1).
Next generation insights with Evosep One
LC-MS based proteomics is not just about individual technologies. It covers the entire workflow from sample preparation to data acquisition – ensuring sensitivity, reliability, and speed. The Evosep One is a standardized platform designed for robustness and high throughput, integrating sample preparation with LC-MS analysis to streamline and optimize your entire workflow – not just individual performance metrics.
Maximum reliability: Designed to keep your research on track with out-of-the-box productivity, providing close to 100% uptime.
Cost-efficient: An affordable solution lovering the cost per sample with 64% and increases MS utilization with 29%.
Patented approach: Innovative sample introduction and separation technology for seamless, efficient operation (US20200041467B1,
US20190250130B1).
INNOVATION DESIGNED TO OVERCOME THE CHALLENGES IN LC-MS BASED PROTEOMICS
Technology developments have been driving the pace of proteomics, pushing the throughput to achieve comprehensive proteomes in less than an hour, enabling large-scale applications and routine workflows. The Evosep One is designed to meet exactly these needs.
The instrument is engineered and built for maximum reliability. By using robust, low-pressure pumps and components to withstand wear and tear from continuous sample elution and gradient formation, it is possible to ensure operation of over tens of thousands of samples.Â
You don’t have to interrupt your analyses to troubleshoot the instrument – you will have a system that is always ready, when you are.
INNOVATION DESIGNED TO OVERCOME THE CHALLENGES IN LC-MS BASED PROTEOMICS
Technology developments have been driving the pace of proteomics, pushing the throughput to achieve comprehensive proteomes in less than an hour, enabling large-scale applications and routine workflows. The Evosep One is designed to meet exactly these needs.
The instrument is engineered and built for maximum reliability. By using robust, low-pressure pumps and components to withstand wear and tear from continuous sample elution and gradient formation, it is possible to ensure operation of over tens of thousands of samples.Â
You don’t have to interrupt your analyses to troubleshoot the instrument – you will have a system that is always ready, when you are.
Short learning curve and ease-of-use
The versatility and ease-of-use of the Evosep One benefits any type of experiment, from multiple, smaller projects to the continuous analysis of large sample cohorts.
You will instantly become an expert on the instrument and achieve cutting-edge performance – simply follow the listed column and emitter specifications to setup the method to confirm performance and reproducibility using the individual method application notes.
Short learning curve and ease-of-use
The versatility and ease-of-use of the Evosep One benefits any type of experiment, from multiple, smaller projects to the continuous analysis of large sample cohorts.
You will instantly become an expert on the instrument and achieve cutting-edge performance – simply follow the listed column and emitter specifications to setup the method to confirm performance and reproducibility using the individual method application notes.
Redefining LC-MS proteomics workflows
Evosep One is different than conventional LC systems for a reason. It integrates sample preparation with LC-MS in a standardized and seamless manner providing ultimate robustness.
The Evotip is the sample introduction device, and is key in this integration through efficient capture and recovery of the sample, providing immediate improvement in the sensitivity of the workflow.
How well does your current setup align with this redefined approach? Are you ready to move beyond individual components and adopt a solution that enhances efficiency, scalability, and performance across your entire proteomics workflow?
webinar  I  on demand
A beginner’s guide to EvosepÂ
Curious about how to get started with the Evosep One or looking for a refresher? Join this beginner-friendly session featuring real user experiences and insights from one of our Product Specialists.
Discover how Evosep One simplifies workflows and supports labs of all sizes.
Innovative sample introduction with the Evotip
The Evotip Pure is the heart of the Evosep technology. It is a precision technology device with extremely high efficiency, integrating desalting with LC-MS sample introduction.
Samples are efficiently concentrated and captured on the Evotip and desalted offline. Once the MS is available, you can simply transfer the box of loaded Evotips to the Evosep One without the need for additional preparation.
The Evosep One will elute all peptides, while contaminants and large hydrophobic molecules remain on the Evotip, which is disposed after each run. This partial elution concept extends the column lifetime, and reduces the amount of carryover, ultimately eliminating the need to use expensive MS time to analyze blank samples.
Innovative sample introduction with the Evotip
The Evotip Pure is the heart of the Evosep technology. It is a precision technology device with extremely high efficiency, integrating desalting with LC-MS sample introduction.
Samples are efficiently concentrated and captured on the Evotip and desalted offline. Once the MS is available, you can simply transfer the box of loaded Evotips to the Evosep One without the need for additional preparation.
The Evosep One will elute all peptides, while contaminants and large hydrophobic molecules remain on the Evotip, which is disposed after each run. This partial elution concept extends the column lifetime, and reduces the amount of carry-over, ultimately eliminating the need to use expensive MS time to analyze blank samples.
Sustainable and scalable workflows
The Evosep One is designed to meet the needs of large-scale LC-MS based proteomics. This is realized through robust instrument design leveraging parallel operation of sample elution and gradient delivery to the analytical column to ensure maximum MS utilization of up to 90%. This robust and continuous operation easily allows for the routine analysis of hundreds of samples daily.
As a result, the bottleneck in high-throughput proteomics workflows has shifted towards sample preparation. The demand for robust, high-throughput sample preparation necessitates standardized and fully automated end-to-end workflows. This can be achieved through miniaturized – yet scalable Evotip-based workflows ensuring a more cost-efficient and sustainable sample preparation.
Versatility through standardized methods
The Evosep One, with its six standard methods, supports a range of use cases from comprehensive proteome analysis to ultra high-throughput single-shot analysis.
It redefines consistency and efficiency in proteomics workflows with optimized methods and dedicated columns, ensuring reproducible performance and simplifying application support.
By minimizing overhead time, it maximizes mass spectrometer utilization, generating more data in less time. Focused on accelerating MS development, it empowers labs to push the boundaries of sensitivity, scalability, and throughput without compromise.
Whisper Zoom methods sets a new standard
Whisper Zoom delivers unparalleled sensitivity for highly versatile applications. These robust methods enable high-throughput analysis for inter-laboratory studies, with a short learning curve that facilitates rapid method adaptation.
This cutting-edge performance addresses the increasing need for scalability, ease-of-use, and robustness, allowing researchers to quickly adopt high-sensitivity workflows with minimal training.
By maximizing recovery and achieving superior chromatographic performance, Whisper Zoom sets a new standard in proteomics research.
On demand webinars
Discover Proteomics at your own pace
Explore our large collection of on-demand webinars, covering everything from beginner guides to advanced workflows with Evosep. In each webinar you can hear directly from our users as they share their research and experiences with the Evosep One.
Additional resources
Get to know Evosep and our technology through the experiences of those using it daily. Discover all publications our users and collaboration partners or dive into our many applications and tech notes.
Instrument Overview
DOWNLOAD OUR MATERIAL
application note
EVOTIP PURE FOR END-TO-END WORKFLOWS: A COST-EFFICIENT, SUSTAINABLE AND SCALABLE PROTEOMICS SOLUTION
application note
EVOTIP PURE BRINGS INNOVATIVE SAMPLE STORAGE AND INTRODUCTION TO HIGH-SENSITIVITY AND SCALABLE LC-MS WORKFLOWS
application note
EVOTIP PURE SIMPLIFIES WORKFLOWS WITH EXCELLENT REPRODUCIBILITY, STORAGE AND RECOVERY
brochure
EVOSEP ONE:
A Standardized Separation
Tool for proteomics
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