EVOSEP ONE: EV-1000Clinical Proteomics must be Sensitive, Fast, and Robust, which Evosep One achieves with perfection
INTEGRATED PURIFICATION AND SEPARATION DELIVERED BY THE EVOSEP ONE
The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads.
Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
Built for standardization
The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.
Illustrated below using the 100 samples per day method and the standard column (EV-1064).
HOW FAST WILL YOU GO?
With the six standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.
The speed of state-of-the-art mass spectrometers combined with the low overhead time and robustness on the Evosep One is a perfect match for high utilization. It is therefore needed to carefully review the balance of proteome depth versus high throughput before starting a series of experiments.
ENHANCED PROTEOME COVERAGE WITH THE PERFORMANCE COLUMN
The performance column is available for the 60 and 100 samples per day methods and is packed with 1.5 μM C18 beads. This reduces the peak widths with 2 seconds at FWHM and leads to an increase in peak capacity of 60%. Consequently, the proteome coverage is enhanced, and the column provides a robust and competitive commercial option – on the same level as home-pulled and packed options, but with better reproducibility and life-time. The boost in sensitivity is particularly noticeable for lower loads, where peptide and protein coverage is improved with 100%.
“Robust and fast workflows are indispensable for successfully implementing large-cohort clinical plasma proteomics studies”,
Professor Matthias Mann, Max-Planck Institute of Biochemistry,
Proteomics and Signal Transduction
A BEginner’s Guide to Evosep
If you are new to our technology or just getting started this event is for you. We will introduce the basics of the Evosep One technology and include hot-from-the-lab data from our users. Nicolai Bache, Head of Applications at Evosep, will host the event, bringing you up to speed with the latest additions to the Evosep One technology.
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