Automated workflows

Automation is vital to enhance efficiency and reproducibility across experiments and support standardization throughout the sample preparation workflow.

Evosep One facilitates robust and reproducible high-throughput analysis of large scale sample cohorts, which is now complemented with our fully automated sample preparation workflow.

 

Webinar
Elevate efficiency: the power of automation

November 09, 2023  I  4:00 PM CET /  10:00 AM EST

In this webinar, we’ll guide you through the process of getting started with automated workflows and highlight the benefits of combining them with the Evosep One.

optimized for your sample loading

Fully automated loading protocol, adaptable to any liquid handling robot  

The workflow is compatible with various liquid handling systems, offering high-throughput sample preparation for proteomics analysis.

This comprehensive process includes a digestion protocol and Evotip Pure loading that demonstrates impressive sensitivity that allows for the identification of numerous proteins from minimal input material. By optimizing the use of consumables, costs associated with proteomics analysis are minimized, improving accessibility.

This efficient approach incorporates liquid layering techniques and positive air pressure to ensure consistent and reliable results, making it highly valuable for a wide range of applications.

automated modular sample preparation strategy

Our new End-to-End Proteomics Workflow offers a comprehensive solution, accelerating discovery while maintaining high data quality and reproducibility. 

It implements a fully automated solution for sample preparation in proteomics research offers significant advantages in terms of efficiency, reproducibility, and productivity. 

A new standard for sample preparation

By implementing a fully automated solution, protocols can be precisely programmed and executed, ensuring reproducibility across different samples and experiments. This minimizes batch effects and enables more reliable data analysis and interpretation.

Enhanced automation significantly reduces manual labor, improving reproducibility and provides researchers with more time for critical decision-making. The workflow serves as a cost-efficient strategy for large-scale applications by carefully considering the needed protein input for digestion. 

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EMPOWER LARGE SCALE PROTEOMICS WITH A MODULAR AUTOMATION STRATEGY

Automated sample prep workflows have gained in popularity in proteomics laboratories by eliminating manual handling steps, reducing human error and variability, leading to more consistent results.

Standardizing the sample preparation adds even more benefits by minimizing batch effects and enables more reliable data analysis and interpretation. The individual protocols can be precisely programmed and executed in different, modular configurations, ensuring reproducibility across different samples and experiments.

Currently available for

Opentrons OT-2

This sample loading protocol for the Opentrons OT-2 offers a robust and rapid end-to-end proteomics workflow for PAC digestion and subsequent digestion protocol for Evotip Pure and can be set up as a fully automated End-to-End workflow.

 A loading kit is required. Find it here.

Agilent AssayMAP Bravo

Here we introduce a complete automated Evotip loading protocol on the Agilent AssayMap Bravo platform. This liquid handler has 96 probe syringes which ensures very fast sample loading on the Evotip – a full box can be loaded in just four minutes.

A loading kit is required. Find it here.

Elevate efficiency

Automation accelerates proteomics, optimizing throughput and reducing costs, making it ideal for labs of all experience levels. With automation you can streamline the entire sample preparation process, especially for large sample volumes or when sensitivity and standardization are crucial.

Free up more time for new discoveries, optimize resources, and tackle larger sample cohorts with ease. Our protocol unleashed efficiency, empowering you to unlock the full potential of your proteomic research.

fast
Optimize throughput, and accelerate data generation

To scale up proteomics, speed is of the essence. Automation has the power to turbocharge data generation and with our loading protocol you are all set for rapid processing, significantly reducing the time required for sample preparation.

Reproducible
consistency and reproducibility across samples

With automation you can minimize the risk of human error, resulting in highly reproducible results. Whether you’re analyzing ten or a hundred samples, you can trust that your data will be consistent, facilitating robust scientific conclusions.

convenient
It’s all in the evotip

With automation comes convenience. The Evotip speeds up your sample loading, reduces carry-over and is a significant contribution to why proteomics has become so easy and routine.

scalable
accommodate larger sample cohorts

The workflow should be scalable and easy to use in any lab. Our protocol is designed with scalability in mind and you can seamlessly process hundreds of samples in a single workday.

Cost-efficient
Automation offers a cost-effective alternative

By optimizing sample usage and reducing reagent consumption, our system streamlines processes while minimizing waste, automation can substantially reduce the overall cost of proteomic analyses.

Easy
One size fits all with standardization

Minimizes the complexity with our user-friendly design. This means that you can easily integrate our protocol into your existing workflows, regardless of your level of expertise.

EVOSEP WEBINAR

MEET OUR USERS and learn how they have implemented automation as part of the sample preparation

Plug’n Play: How the Evotip facilitates simple, affordable, end-to-end, automated sample preparation workflows

November 09, 2023  I  4:00 PM CET /  10:00 AM EST

In this webinar, we’ll guide you through the process of getting started with automated workflows and highlight the benefits of combining them with the Evosep One.

Automated sample prep workflows

available on demand

In this webinar, we will focus on automated samples prep workflows. Users will share how they have standardized their sample prep workflows and discuss the advantages of combining automated sample prep workflows with the Evosep One.

More research using an automated sample prep workflow

Here you can see publications available using an automated sample preparation workflow featuring Evosep One. For a full overview of publications published using the Evosep One Technology visit our Literature room here

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
MassSpecPreppy—An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications2023

Read publication here

Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel, , , 2023

Read publication here

End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-22023The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instru- ment per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation. To address this, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is …,

Download application note here

Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo2023Automation and robotics have revolutionized the field of proteomics, offering solutions to the growing need for streamlined workflows and efficient sample processing. In this context, automated loading of Evotips has emerged as a pivotal advancement in sample preparation workflows. The Evotip can now be loaded using an innovative and fully automated strategy with the Agilent …, ,

Download application note here

A complete and automated sample preparation strategy for high-throughput and standardized proteomics, applied to a clinical cohort of patient plasma samples2022,

Read poster here

Automated sample prep workflows2021In this webinar, we will focus on automated samples prep workflows. Users will share how they have standardized their sample prep workflows and discuss the advantages of combining automated sample prep workflows with the Evosep One.

Watch webinar

Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures, , , , , 2021In this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1 inhibition in T-cell acute lymphoblastic leukemia.,

Read publication here

Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids, , , 2021In this seminar from the Canadian National Proteomics Network (CNPN) meeting 2021, Angela McArdle presents a standardized workflow for precise high-throughput proteomics of blood biofluids. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood.

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Learn more

High-resolution longitudinal serum proteome trajectories in COVID-19 reveal patients-specific seroconversion, , 2021This publication from OmicEra Diagnostics, describes alterations of the serum proteome during COVID-19 using a scalable plasma proteome profiling workflow. It comprises the most detailed longitudinal protein trajectories during hospitalization, based on one of the largest MS-based body fluid proteomics efforts with a total of 720 plasma serum samples.

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho., ,

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Evosep application note for AssayMAP bravo protocol for Evotip
Application Note
End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2
Evosep application note for AssayMAP bravo protocol for Evotip
Application Note
Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo
application note
Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
brochure
EVOSEP ONE: A Standardized Separation
Tool for Clinical Omics
application note
A complete, automated Opentrons OT-2 loading protocol
for simplified sample loading of Evotip Pure