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High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe

by Eva Bryderup

The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the...

Application of diaPASEF for high-throughput and high-sensitivity proteomics with the Evosep One

by Eva Bryderup

Proteomics workflows have become increasingly successful in the characterization of complex proteomes in great depth. Application to large sample cohorts requires a high degree of reproducibility and data completeness, which makes DIA workflows interesting.

diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition

by Eva Bryderup

A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data...

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    Webinar: High-throughput drug discovery with the Evosep One
    30/9/20214:00pm Register
    Webinar: Automated sample prep workflows
    28/10/20214:00pm Register
    Webinar: FFPE tissue analysis on the Evosep One
    24/11/20214:00pm Register

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    Webinar: Whisper is coming

    Whisper is Here

    15/12/2020j/n/Y4:00pm CET2020-12-15T15:00:00Zg:ia T1 hour

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    Sorry, your location could not be determined.Fill in your information to watch the recorded version of our webinar where you will learn how the pioneering Whisper™ flow technology is driving new Evosep One applications. When you register for the recording you will also join our monthly newsletter. You can unsubscribe at any time.

    In this webinar, we will introduce our next addition to our dynamic environment of specialized applications, EVOSEP+. We are developing a set of nanoflow methods for standard Evosep One hardware, which builds on a robust platform allowing for several thousand uninterrupted injections at very low flow rates. We will demonstrate the expected increase in sensitivity and benchmark the performance directly against our established standard methods.

     

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    • Events
      • Conferences
      • webinars
    • Shop
    • products
      • Evosep One
      • Evotip
      • Columns and emitters
    • Methods
      • Standard methods
        • 300 SPD
        • 200 SPD
        • 100 SPD
        • 60 SPD
        • 30 SPD
      • EVOSEP+
        • Extended method
        • High organic method
        • Whisper 40SPD
        • Whisper 20SPD
    • Applications
      • COVID-19
      • DIA
      • Host cell protein
      • PLASMA
      • PROTEIN-PROTEIN INTERACTIONS
      • TARGETED WORKFLOWS
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      • Literature room
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    • Support
    • About
      • Why Evosep?
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