Whisper Zoom methods for high-sensitivity applications

Whisper Zoom methods
for high-sensitivity applications

Whisper Zoom for high-sensitivity applications through standardization and ease-of-use

Our Whisper Zoom methods drives scalable workflows for high-sensitivity applications through standardisation and ease-of-use.

At the moment the Whisper Zoom methods covers throughputs from 20 to 120 samples per day to scale high-sensitivity proteomics including SCP,
deep visual proteomics (DVP) and phosphoproteomics workflows.

While expanding our scope for these methods, we continue to deliver these with our key values such as ease of use, speed, robustness and sensitivity.

Whisper zoom methods

The Whisper Zoom methods are specifically designed to significantly improve and maximize recovery of the sample with highly optimized flow paths to deliver sensitivity and robustness with unmatched performance. Here you can dive into the unique advantages of each of the Whisper Zoom methods.

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Exceptional sensitivity and scalable throughput with up to 120 samples per day

Whisper Zoom methods benefit significantly from the enhanced chromatographic performance delivered by the Evosep Eno.

By enabling sensitivity and throughput, these methods support the discovery of biomarkers, deep phenotyping, and detailed molecular characterization, accelerating translational research and expanding access to high-quality proteomics for a broad range of laboratories and studies.

high sensitivity for cutting-edge LC-MS proteomics

Our Whisper Zoom methods are engineered to deliver unmatched sensitivity by minimizing sample loss and optimizing every step of the LC-MS workflow.

With highly refined flow paths, advanced Evotip technology, and robust, standardized protocols, Whisper Zoom ensures maximum sample recovery and sharp, well-defined chromatographic peaks.

This means you can confidently achieve deeper proteome coverage, even at ultra-low sample inputs, while enjoying the ease-of-use and reproducibility needed for high-throughput, high-sensitivity applications. Whisper Zoom transforms sensitivity from a technical challenge into a reliable, accessible standard for every lab.

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EVOTIP BRINGS INNOVATIVE SAMPLE STORAGE INTRODUCTION TO HIGH-SENSITIVITY LC-MS WORKFLOWS

The Evotip, a disposable trap column, is the core of our technology. The Evotip serves as an integrated purification step, where contaminants are left on the tip, and analytically relevant peptides are eluted.

The Evotip is extraordinary important for ultra-sensitive analysis, as very small sample amounts can be loaded in higher volumes.

Furthermore, loaded Evotips can be stored cold until analysis, which is especially important for single cell proteomics, where very limited sample amount sets higher demands to the storage of peptide and become a crucial factor for reproducible analysis.

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Efficient SAMPLE CAPTURE AND RECOVERY ALLOWS FOR SENSITIVE ANALYSIS

In contrast to other omics technologies, a prerequisite for proteomics is to get the best results possible from the starting material available. This sets high requirements to minimizing losses – a challenge throughout complex sample preparation workflows as each sample transfer step entails a source of variation and sample loss through peptide adsorption and loss of sample volume.

The Evotip reduces the number of sample transfer steps compared to alternative strategies, but still benefits from using n-dodecyl-β-D-maltoside(DDM) for low sample loads until saturation is observed at higher loads. As peptides are stored equally well on the Evotip regardless of using DDM, the beneficial effect from DDM impacts the sample preparation workflow upstream of the Evotip.

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MAXIMIZE THROUGHPUT AND PERFORMANCE WITH WHISPER ZOOM

High-performing liquid chromatography is essential for sensitive proteomics, relying on the precise separation of analytes into narrow, well-defined peaks.

By concentrating peptides into tight elution windows, Whisper Zoom methods reduce co-elution and enhance separation power, enabling the resolution of more peptides within each run.

Fast and precise hardware synchronization ensures robust and consistent results, even with low sample inputs where minor losses can significantly impact proteome coverage. Whisper Zoom methods demonstrate outstanding retention time stability and peak capacity, providing a strong foundation for reproducible, high-sensitivity proteomics workflows.

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