Innovative sample introduction for robust and reproducible LC-MS workflows
Evosep Eno stands apart from conventional LC systems. It integrates sample preparation with LC-MS in a standardized and seamless manner, providing enhanced robustness. Evotip Pure, as the sample introduction device, is key to this integration. It efficiently captures and recovers samples, immediately improving the sensitivity of the workflow.
With Evotip Pure you get:
- High efficiency disposable trap column
- Innovative sample storage
- Cost-efficiency by minimized use of reagents and longer column lifetime
- Workflow flexibility
High efficiency disposable trap colulmn
The high-efficiency disposable trap column in the Evosep system significantly reduces overhead time and increases sample throughput.
This makes workflows more efficient and allows for the analysis of larger sample sets in less time. The Evotip technology simplifies and enhances the proteomic analysis of minimal cell quantities and liquid biopsies, setting a new standard in sensitivity, throughput, and robustness.Â
- 20% increased recovery through fewer transfer steps
- Up to 80% utilization of the processed sample
Explore the benefits and see how efficient sample capture and recovery
streamlines high-sensitivity workflows.
Streamlined workflow integrates sample preparation with peptide elution
The Evotip is a high precision and well characterized technology, that simplifies sample preparation by seamlessly integrating desalting – which is typically required in bottom-up proteomics workflows – with LC-MS sample introduction.Â
All analytically relevant peptides are eluted from the Evotip by the Evosep Eno, while retaining other large and more hydrophobic molecules that are discarded with the single-use, disposable Evotip. This methodology ensures high system robustness, excellent run-to-run reproducibility, and completely eliminates carryover from the injection system.
The Evotip allows you to:
- Protect your samples through immediate sample loading
- 28-day storage window for peptides until LC-MS analysis
- A hands-off approach for automated workflow integration
A GREEN TRANSITION FOR LC-MS BASED PROTEOMICS
The Evotip simplifies sample handling from digestion to separation, reducing the number of single-use plastic tips needed to load a single sample.
The Evotip allows the Evosep Eno to handle up to 500 samples per day, using 79% less solvent and 35% less plastic per sample compared to a traditional nLC. During sample preparation, the sample is loaded onto the Evotip, eliminating the need for extra consumables like vials, pipette tips, and solvents for elution and re-suspension.
Additionally, the minimal carryover of Evotips reduces or eliminates the necessity for blank runs during experiment setup, leading to significant savings in liquid organic waste and electricity usage.Â
No carryover with the Evotip, even for challenging sample types
Scaling plasma proteomics to thousands of patient samples demands workflows that are not only robust and reproducible but also cost-efficient and contamination-free.
In our latest application note, we demonstrate how Evosep-based workflows powered by Evotip Pure enable the analysis of over 1,100 plasma samples in under 7 days with ultra-low carryover (<0.2%), even in complex matrices. The disposable Evotip design safeguards the LC system and ensures consistent performance across large cohorts, eliminating cross-contamination and preserving sample integrity.
This study highlights how automation, miniaturization, and smart integration with Evosep make large-scale plasma proteomics not just accessible, but reliable and scalable.
Innovative Sample storage
The Evotip system is designed to protect your analytical column by reducing the complexity and time required for sample preparation. This not only extends the lifespan of your column but also ensures consistent and reliable results.
The Evotip workflow enables the seamless, cost-effective identification of proteins from biological matrices like blood plasma. By minimizing sample handling, the Evotip reduces variability and loss. Especially those caused by peptide adsorption and volume changes during transfer.
This streamlined approach supports sensitive and reproducible analysis, even at low sample loads. And with the ability to store samples directly on the Evotip for up to 28 days at 4 °C, it becomes a powerful tool for scaling experiments and coordinating large batches ahead of LC-MS analysis.
Improved column longevity powered by the Evotip Pure
The Evosep Eno is built for standardization – this means fixed methods with specified columns – setting you up for immediate success.
The key to this lies in the nature of the Evotip Pure, which functions as a disposable trap column that safeguards the LC system and chromatographic column. This becomes crucial when analyzing more challenging sample types like body fluids, FFPE or tissue samples by combining the clean-up step with partial elution of the peptides of interest.Â
This is demonstrated through exceptional stability and reliability for large-scale plasma proteomics, processing ~400 sample injections with a consistent pressure profile (<1% CV) and no systematic pressure build-up, confirming its suitability for continuous high-throughput operation.
AUTOMATED EVOTIP LOADING DRIVES SCALABLE PROTEOMICS WORKFLOWS
Automated Evotip loading is the cornerstone of an easy-to-implement solution for automated end-to-end proteomics workflows, combining scalability, cost-efficiency, and sustainability in a single integrated platform.
By streamlining sample preparation, minimizing peptide loss, and improving sample recovery, automated Evotip loading allows efficient processing of large sample cohorts with minimal manual intervention.
By integrating Evotip Pure directly in a complete, automated end-to-end workflows, you achieve:
- Reduced manual handling
- Reproducible results
- Lower CV’s for higher data quality
high sensitivity workflows streamlined by Efficient sample capture and recovery
Maximizing sensitivity while minimizing sample loss is essential for reproducible proteomics. Evotip Pure enables efficient concentration of low-input samples, making it ideal for high-sensitivity LC-MS workflows, including single-cell proteomics.
In benchmark studies, Evotip Pure demonstrated exceptional performance in capturing and releasing peptides from low-input samples, with minimal carryover and high reproducibility. The format supports direct loading into Evosep workflows, reducing sample handling and preserving integrity throughout the process.
By integrating sample storage and elution into a single device, Evotip Pure simplifies the path to high-sensitivity LC-MS, enabling researchers to confidently explore low-abundance proteomes with scalable throughput.
CASE STUDY I RAPID NOVOR
EVOTIP ENHANCES EFFICIENCY AND SENSITIVITY IN CLINICAL PROTEOMICS
At Rapid Novor, the integration of Evotips into their LC-MS workflow is transforming patient care. Their innovative diagnostic test, EasyM, is significantly improving the lives of patients with multiple myeloma—a blood cancer that demands precise monitoring and treatment.
By incorporating Evotips, Zac McDonald and his team at Rapid Novor have replaced invasive biopsies with accessible serum-based methods, enabling fast and sensitive disease monitoring. The use of Evotips has reduced overhead by 80%, shortened LC-MS runtime by 30%, and doubled throughput, all while maintaining sample stability at room temperature for 48 hours.Â
webinar  I  available on demand
Evosep Eno: A New Era of Speed, Scale and Robustness
Discover how Evosep Eno simplifies workflows and supports labs of all sizes.
The Evosep Eno represents a significant leap forward in proteomics technology — delivering the speed, precision, and reproducibility needed to transform drug discovery and translational research.
Additional resources
Get to know Evosep and our technology through the experiences of those using it daily. Discover all publications our users and collaboration partners or dive into our many applications and tech notes.
LEarn more about the Evotip
Nicolai Bache, VP Proteomics Research at Evosep, elaborates on the key features and explains why the Evotip is the perfect match for high sensitivity workflows such as single cell and plasma proteomics.
The Evotip may seem like a simple device, but in reality, it’s a precision technology with extremely high efficiency.
To learn more about the basics of our technology and a more detailed introduction of the Evotip view our Beginners guide webinar on demand here
DOWNLOAD OUR MATERIAL
Application note
 Evotip Pure brings innovative sample storage and
introduction to high-sensitivity and scalable LC-MS workflows
BROCHURE
evosep Eno: a standardized separation tool for proteomics
Application Note
 Evotip Pure simplifies workflows with excellent
reproducibility, storage and recoveryEVOTIPS
quick guide
Evotip pure
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