MS connections - Evosep Eno
Here you can learn more about the different compatibility options available for the Evosep Eno.Have a technical issue? Contact support or access online troubleshooting
MS Connection
The Evosep Eno is compatible with a wide range of Mass Spectrometers (MS). In addition to the simple hardware setup described on this page, software control can be fully integrated into their Chromatography Data System (CDS) Software.
Seamless Integration: Whatever your setup, Evosep Eno can easily interface with your MS of choice. Recommended Evosep Eno column oven solutions for MS ionization sources are specified below.
Optimal Performance: Evosep Eno with specified column heating solutions ensures accurate temperature regulation of the analytical column during LC-MS analysis, yielding reliable and reproducible results.
Evosep Eno promotes standardization in chromatography by facilitating a defined connection setup for each MS interface, making high-quality chromatography easier, more reliable, and effortlessly integrated into your lab.
Standard methods
Our standard methods on the Evosep Eno supports a wide range of mass spectrometry sources. See the overview in this section.
Bruker CaptiveSpray
The Evosep Eno is compatible with CaptiveSpray, CaptiveSpray 2 and CaptiveSpray Ultra ionization sources, when equipped with a column toaster. The setup for EV1182, EV1109, and EV1137 Performance Columns requires Captive Spray Emitters and union available from Bruker (181107 Captive Spray 2 Emitter (ZDV) 20 µm ID, 8204500 Stainless steel union (1/16″-through-hole union with hex handle) and 1865691 Captive Spray Emitter (ZDV) 10 µm ID).
Thermo EASY-Spray Connection with Evosep Pod
Evosep Pod for Thermo EASY-Spray (EV1187) is a column oven designed specifically for Evosep Performance Columns when used with the Thermo Scientific ™ EASY-Spray ™ ionization source. It is compatible with EV1182, EV1109, and EV1137 Performance Columns.
The Thermo Scientific EASY-Spray adapter (EV1072, included with Evosep Pod) connects the analytical focusing column to a replaceable emitter with an integrated ZDV liquid junction inside the high-voltage click and spray mechanism. It is used with the Thermo Scientific EASY-Spray ion source (ES082) and Evosep emitters (EV1086, EV1087 and EV1111).
Thermo Nanospray Flex connection with Evosep Pod
Evosep Pod for Thermo Nanospray Flex (EV1194) is a column oven designed for Evosep Performance Columns when used with the Thermo Scientific ™ Nanospray Flex ™ ionization source and the Evosep Eno. It is compatible with EV1182, EV1109, and EV1137 Performance Columns.
The Adapter (EV1192, included with Evosep Pod) connects the analytical focusing column to a replaceable emitter with an integrated ZDV liquid junction. It is used with the Thermo Scientific Nanospray Flex ion source (ES071, ES072) and Evosep emitters (EV1086, EV1087 and EV1111).
Sciex OptiFlow
The Evosep Eno is compatible with the SCIEX OptiFlow™ Turbo V ionization source equipped with a column heater. The setup for EV1182, EV1109, and EV1137 Performance Columns requires SteadySpray Electrode Emitters available from SCIEX (5061574 SteadySpray Electrode Low Micro 1-10µL/min) and a NanoTight Union (IDEX P/N P-779-01).
Waters Nanolockspray Connection with
Evosep Pod
The Evosep Pod for Waters NanoLockSpray (EV1189) is a column oven designed for Evosep Performance Columns when used with the Waters™ NanoLockSpray ionization source and the Evosep Eno. It is compatible with EV1182, EV1109, and EV1137 Performance Columns.
The Adapter (EV1192, included with Evosep Pod) connects the analytical focusing column to a replaceable emitter with an integrated ZDV liquid junction inside the Evosep Pod. It is used with the Waters NanoLockSpray ion source and Evosep emitters (EV1086, EV1087 and EV1111).
Agilent NanoSpray ESI connection with
Evosep Pod
The Evosep Pod for Agilent Nanospray (EV1191) is a column oven designed for Evosep Performance Columns when used with the Agilent Nanospray ESI source and the Evosep Eno. It is compatible with EV1182, EV1109, and EV1137 Performance Columns.
The Adapter (EV1193, included with Evosep Pod) connects the analytical focusing column to a replaceable emitter with an integrated ZDV liquid junction inside the Evosep Pod. It is used with the Agilent Nanospray ion source (G1992A) and Evosep emitter (EV1117).
Whisper Zoom methods
Our specialized methods on the Evosep Eno supports a wide range of mass spectrometry sources. See the overview in this section.
Bruker CaptiveSpray
The Evosep One is compatible with the Bruker CaptiveSpray, CaptiveSpray 2, and CaptiveSpray Ultra ion sources, when equipped with a Column Toaster. Whisper Zoom methods use IonOpticks Aurora® columns with integrated pulled emitters, which connect directly to the CaptiveSpray source, and are temperature-controlled using the Bruker Column Toaster.
The Whisper Zoom 20 SPD and 40 SPD methods both use the Aurora® Elite™ 15 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-150150C18-CSI).
The Whisper Zoom 80 SPD and 120 SPD methods both use the Aurora® Rapid™ 5 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-5075C18-CSI).
Thermo EASY-Spray
For Whisper Zoom methods, IonOpticks Aurora® columns with integrated pulled emitters are used together with the Thermo Scientific™ EASY-Spray™ ionization source. These columns are temperature-controlled using the IonOpticks HeatSync™ column heater.
The Whisper Zoom 20 SPD 40 SPD methods both use the Aurora® Elite™ 15 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-150150C18).
The Whisper Zoom 80 SPD and 120 SPD methods both use the Aurora® Rapid™ 5 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-5075C18).
The IonOpticks HeatSync™ column heater is required for all Whisper Zoom configurations, and when using the 5 cm Aurora® Rapid™ columns for Whisper Zoom 80 SPD or 120 SPD, the dedicated HeatSync™ 5 cm column housing is additionally required.
Thermo Nanospray Flex
For Whisper Zoom methods, IonOpticks Aurora® columns with integrated pulled emitters are used together with the Thermo Scientific™ Nanospray Flex™ ionization source. The columns are positioned in a nanospray holder on the ionization source.
The Whisper Zoom 20 SPD and 40 SPD methods both use the Aurora® Elite™ 15 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-150150C18).
The Whisper Zoom 80 SPD and 120 SPD methods both use the Aurora® Rapid™ 5 cm × 75 µm, 1.7 µm column (IonOpticks PN: AUR4-5075C18).
The IonOpticks HeatSync™ column heater is required for all Whisper Zoom configurations, and when using the 5 cm Aurora® Rapid™ columns for Whisper Zoom 80 SPD or 120 SPD, the dedicated HeatSync™ 5 cm column housing is additionally required.
