Although the name and throughput of the Evosep Eno methods match those for the Evosep One, the methods with their set columns have been optimized for each instrument and are not identical. Evosep Eno offers improved chromatographic performance compared to Evosep One, see the brochure and method application notes for details https://www.evosep.com/evosep-eno/. Note that a column oven is required for all Evosep Eno methods and selected Evosep One methods.