Evosep has optimized their Evosep Eno methods for the specified columns for each method. This means that the best peptide chromatography performance and robustness will be achieved with the specified columns. Uniquely, Evosep guarantees the performance of the system with the specified column matches their method application note for a digest standard and supports users to achieve this. Evosep cannot guarantee performance or support any other columns. Details on the specified column for each method is available at https://www.evosep.com/columns-and-emitters/  together with links to the standard method application notes.