Find answers to the most asked questions below.

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Evosep One

No, methods are fixed for optimal performance.

Yes. By using the soak adaptor with part no EV1068 and the Thermo Scientific Nunc deep well plate with part no. 260251 it is possible to collect up to 500 µl from each tip.

With this insert you can collect 500 µl of flow through.

No, once used the plastic in the Evotips will be slightly deformed and cannot be used again. Doing so will void instrument warranty.

We do not recommend using other type of columns. The Evosep One methods are optimized for Evosep One columns.

No, the Evosep One is accurately designed for a perfect low-pressure, leak-free fit with Evotips. Using other pipette tips will void instrument warranty.

No, Evosep One methods are optimized using 100% water as solvent A and 100% Acetonitrile as Solvent B (both with 0.1% formic acid, both in LC/MS grade quality).

Add the “Prepare flow to column, Idle flow” script to the queue.

In Windows 10 go to the menu called “App & features” and click the Evosep One Chronos Plugin icon. On older Windows versions the info can be found under programs & features.

No, direct infusion is not possible with the Evosep One system.

No, Evotips are only available with C18 material.

It varies from method to method, please see the table below.


We recommend loading 20ul of sample, but volume can be changed if the loading protocol is adapted accordingly (higher volume => Longer centrifugation time, lower volume => shorter centrifugation time)

The stationary phase of the Evotip is C18.

The symmetry factor of especially the hydrophilic peptides are slightly affected and show tailing because the Evosep One methods are optimized for the highest number of peptide identifications and most efficient utilization of the mass spectrometer (elution window). 

Please visit the Evosep Support Zone, which holds newest software releases, manuals etc. or the Evosep One product page for application notes on the standard methods etc.

Available methods are shown in the table below:

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